Gene description for PCLO
Gene name piccolo presynaptic cytomatrix protein
Gene symbol PCLO
Other names/aliases ACZ
Species Homo sapiens
 Database cross references - PCLO
ExoCarta ExoCarta_27445
Entrez Gene 27445
HGNC 13406
MIM 604918
UniProt Q9Y6V0  
 PCLO identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for PCLO
Molecular Function
    calcium-dependent phospholipid binding GO:0005544 ISS
    calcium ion binding GO:0005509 ISS
    profilin binding GO:0005522 ISS
Biological Process
    cytoskeleton organization GO:0007010 ISS
    synapse assembly GO:0007416 IEA
    cAMP-mediated signaling GO:0019933 IEA
    regulation of exocytosis GO:0017157 IEA
    insulin secretion GO:0030073 IEA
    synaptic vesicle exocytosis GO:0016079 NAS
Subcellular Localization
    cell junction GO:0030054 IEA
    cytoskeleton GO:0005856 NAS
    synapse GO:0045202 ISS
    membrane GO:0016020 IEA
    presynaptic cytoskeletal matrix assembled at active zones GO:0048788 TAS
    postsynaptic density GO:0014069 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified PCLO in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for PCLO
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PCLO is involved
No pathways found





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