Gene description for FBXW8
Gene name F-box and WD repeat domain containing 8
Gene symbol FBXW8
Other names/aliases FBW6
FBW8
FBX29
FBXO29
FBXW6
Species Homo sapiens
 Database cross references - FBXW8
ExoCarta ExoCarta_26259
Vesiclepedia VP_26259
Entrez Gene 26259
HGNC 13597
MIM 609073
UniProt Q8N3Y1  
 FBXW8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for FBXW8
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    Golgi organization GO:0007030 IGI
    Golgi organization GO:0007030 ISS
    cell population proliferation GO:0008283 IDA
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    positive regulation of dendrite morphogenesis GO:0050775 IDA
    positive regulation of dendrite morphogenesis GO:0050775 ISS
    cilium assembly GO:0060271 IBA
    labyrinthine layer blood vessel development GO:0060716 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    centriole GO:0005814 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IEA
    Cul7-RING ubiquitin ligase complex GO:0031467 IBA
    Cul7-RING ubiquitin ligase complex GO:0031467 IDA
    ciliary basal body GO:0036064 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    3M complex GO:1990393 IDA
 Experiment description of studies that identified FBXW8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for FBXW8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL26L1 51121
Affinity Capture-MS Homo sapiens
2 MAGEA10  
Affinity Capture-MS Homo sapiens
3 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ARID5B 84159
Affinity Capture-MS Homo sapiens
5 TTBK1  
Affinity Capture-MS Homo sapiens
6 SPRED2 200734
Affinity Capture-MS Homo sapiens
7 FBXO4 26272
Affinity Capture-Western Homo sapiens
8 SNX33 257364
Affinity Capture-MS Homo sapiens
9 MTPAP 55149
Affinity Capture-MS Homo sapiens
10 ACTC1 70
Affinity Capture-MS Homo sapiens
11 MRFAP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 MPO 4353
Affinity Capture-MS Homo sapiens
13 CNP 1267
Affinity Capture-MS Homo sapiens
14 CCT6B 10693
Affinity Capture-MS Homo sapiens
15 FER1L4  
Affinity Capture-MS Homo sapiens
16 MRPL52  
Affinity Capture-MS Homo sapiens
17 RPUSD4 84881
Affinity Capture-MS Homo sapiens
18 SEMA4D  
Affinity Capture-MS Homo sapiens
19 LUZP1 7798
Affinity Capture-MS Homo sapiens
20 C17orf98  
Affinity Capture-MS Homo sapiens
21 DIAPH1 1729
Affinity Capture-MS Homo sapiens
22 ZNF408  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 COX11  
Affinity Capture-MS Homo sapiens
24 G3BP2 9908
Affinity Capture-MS Homo sapiens
25 CTBS 1486
Affinity Capture-MS Homo sapiens
26 FBXW8 26259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MAPK14 1432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 DISP1 84976
Affinity Capture-MS Homo sapiens
29 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PHLDB2 90102
Affinity Capture-MS Homo sapiens
31 BRDT  
Affinity Capture-MS Homo sapiens
32 CTRL  
Affinity Capture-MS Homo sapiens
33 PSMB6 5694
Affinity Capture-MS Homo sapiens
34 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 BTRC 8945
Affinity Capture-Western Homo sapiens
36 PSMA1 5682
Affinity Capture-MS Homo sapiens
37 PAPL  
Affinity Capture-MS Homo sapiens
38 VWA7  
Affinity Capture-MS Homo sapiens
39 TWF1 5756
Affinity Capture-MS Homo sapiens
40 C2orf88 84281
Affinity Capture-MS Homo sapiens
41 KIF14 9928
Affinity Capture-MS Homo sapiens
42 EXOSC9 5393
Affinity Capture-MS Homo sapiens
43 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 NANOG  
Affinity Capture-Western Homo sapiens
45 ZKSCAN8  
Affinity Capture-MS Homo sapiens
46 TCP1 6950
Affinity Capture-MS Homo sapiens
47 PTPRN2  
Affinity Capture-MS Homo sapiens
48 IGKV2D-28 28883
Affinity Capture-MS Homo sapiens
49 RBX1 9978
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
50 L1RE1  
Affinity Capture-MS Homo sapiens
51 CCT5 22948
Affinity Capture-MS Homo sapiens
52 LARP4 113251
Affinity Capture-MS Homo sapiens
53 GCC2 9648
Affinity Capture-MS Homo sapiens
54 FAM222A  
Affinity Capture-MS Homo sapiens
55 PDCD5 9141
Affinity Capture-MS Homo sapiens
56 SIPA1L2  
Affinity Capture-MS Homo sapiens
57 CCND1 595
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
58 CCDC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 YWHAQ 10971
Affinity Capture-MS Homo sapiens
60 TTC9C 283237
Affinity Capture-MS Homo sapiens
61 UBE2D3 7323
Reconstituted Complex Homo sapiens
62 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 REST 5978
Affinity Capture-Western Homo sapiens
64 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
65 ATP5B 506
Affinity Capture-MS Homo sapiens
66 H3F3C 440093
Affinity Capture-MS Homo sapiens
67 LRCH3 84859
Affinity Capture-MS Homo sapiens
68 MAP4K1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 CETN1  
Affinity Capture-MS Homo sapiens
70 TRIP13 9319
Affinity Capture-MS Homo sapiens
71 RBBP6 5930
Affinity Capture-MS Homo sapiens
72 TMPO 7112
Affinity Capture-MS Homo sapiens
73 GOLGA3 2802
Affinity Capture-MS Homo sapiens
74 FHOD3  
Affinity Capture-MS Homo sapiens
75 ARHGAP10 79658
Affinity Capture-MS Homo sapiens
76 DGKG  
Affinity Capture-MS Homo sapiens
77 XPO1 7514
Affinity Capture-MS Homo sapiens
78 NCBP2 22916
Affinity Capture-MS Homo sapiens
79 CCT6A 908
Affinity Capture-MS Homo sapiens
80 LAMB2 3913
Affinity Capture-MS Homo sapiens
81 NFKBIA  
Affinity Capture-Western Homo sapiens
82 MRPS14  
Affinity Capture-MS Homo sapiens
83 SKP2  
Affinity Capture-Western Homo sapiens
84 BCL3  
Affinity Capture-Western Homo sapiens
85 CALM1 801
Affinity Capture-MS Homo sapiens
86 VBP1 7411
Affinity Capture-MS Homo sapiens
87 NR5A2  
Affinity Capture-MS Homo sapiens
88 YTHDC1  
Affinity Capture-MS Homo sapiens
89 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
90 PCLO 27445
Affinity Capture-MS Homo sapiens
91 DNAJC9 23234
Affinity Capture-MS Homo sapiens
92 NLRP1  
Affinity Capture-MS Homo sapiens
93 CBR4  
Affinity Capture-MS Homo sapiens
94 AP2A2 161
Affinity Capture-MS Homo sapiens
95 HSPA4 3308
Affinity Capture-MS Homo sapiens
96 DDB1 1642
Affinity Capture-Western Homo sapiens
97 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 LRRC53  
Affinity Capture-MS Homo sapiens
99 MMP9 4318
Affinity Capture-MS Homo sapiens
100 ZC2HC1C  
Affinity Capture-MS Homo sapiens
101 DLAT 1737
Affinity Capture-MS Homo sapiens
102 PFDN5 5204
Affinity Capture-MS Homo sapiens
103 ZDHHC15 158866
Affinity Capture-MS Homo sapiens
104 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 RBBP4 5928
Affinity Capture-MS Homo sapiens
106 FBLN5 10516
Affinity Capture-MS Homo sapiens
107 ZNF669  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 FBXW2  
Affinity Capture-Western Homo sapiens
109 FASN 2194
Affinity Capture-MS Homo sapiens
110 TBC1D3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
111 MYL6 4637
Affinity Capture-MS Homo sapiens
112 PDCL3 79031
Affinity Capture-MS Homo sapiens
113 MAP2 4133
Affinity Capture-MS Homo sapiens
114 CUL2 8453
Affinity Capture-MS Homo sapiens
115 TP53 7157
Affinity Capture-MS Homo sapiens
116 DOCK5 80005
Affinity Capture-MS Homo sapiens
117 UGT2B17  
Affinity Capture-MS Homo sapiens
118 ERLIN1 10613
Affinity Capture-MS Homo sapiens
119 KEAP1 9817
Affinity Capture-MS Homo sapiens
120 SKP1 6500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
121 EIF5A2 56648
Affinity Capture-MS Homo sapiens
122 G3BP1 10146
Affinity Capture-MS Homo sapiens
123 UTP23  
Affinity Capture-MS Homo sapiens
124 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
125 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 GORASP1 64689
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
127 ATAD3A 55210
Affinity Capture-MS Homo sapiens
128 SNRNP40 9410
Affinity Capture-MS Homo sapiens
129 CAD 790
Affinity Capture-MS Homo sapiens
130 TTC29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 RPUSD3  
Affinity Capture-MS Homo sapiens
132 MRPS16  
Affinity Capture-MS Homo sapiens
133 STK11 6794
Affinity Capture-MS Homo sapiens
134 GLYR1 84656
Affinity Capture-MS Homo sapiens
135 GPR135  
Affinity Capture-MS Homo sapiens
136 CUL7 9820
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
137 CCDC67  
Affinity Capture-MS Homo sapiens
138 PSMD3 5709
Affinity Capture-MS Homo sapiens
139 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
140 IRS1 3667
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
141 PCNP 57092
Affinity Capture-MS Homo sapiens
142 CUL4A 8451
Affinity Capture-Western Homo sapiens
143 EP300 2033
Affinity Capture-Western Homo sapiens
144 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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