Gene description for MPO
Gene name myeloperoxidase
Gene symbol MPO
Other names/aliases -
Species Homo sapiens
 Database cross references - MPO
ExoCarta ExoCarta_4353
Vesiclepedia VP_4353
Entrez Gene 4353
HGNC 7218
MIM 606989
UniProt P05164  
 MPO identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for MPO
Molecular Function
    chromatin binding GO:0003682 TAS
    peroxidase activity GO:0004601 IBA
    peroxidase activity GO:0004601 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    heme binding GO:0020037 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    response to yeast GO:0001878 IEA
    hypochlorous acid biosynthetic process GO:0002149 IEA
    respiratory burst involved in defense response GO:0002679 IEA
    defense response GO:0006952 TAS
    response to oxidative stress GO:0006979 TAS
    response to mechanical stimulus GO:0009612 IEA
    removal of superoxide radicals GO:0019430 IEA
    response to food GO:0032094 IEA
    response to lipopolysaccharide GO:0032496 IEA
    low-density lipoprotein particle remodeling GO:0034374 IDA
    defense response to bacterium GO:0042742 IBA
    hydrogen peroxide catabolic process GO:0042744 IDA
    hydrogen peroxide catabolic process GO:0042744 IDA
    negative regulation of apoptotic process GO:0043066 TAS
    defense response to fungus GO:0050832 IEA
    response to gold nanoparticle GO:1990268 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 HDA
    nucleoplasm GO:0005654 IDA
    lysosome GO:0005764 TAS
    secretory granule GO:0030141 IDA
    azurophil granule lumen GO:0035578 TAS
    azurophil granule GO:0042582 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
    phagocytic vesicle lumen GO:0097013 TAS
 Experiment description of studies that identified MPO in exosomes
1
Experiment ID 494
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MPO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PHF21B  
Affinity Capture-MS Homo sapiens
2 CAT 847
Co-fractionation Homo sapiens
3 PRTN3 5657
Affinity Capture-MS Homo sapiens
4 RNF40 9810
Affinity Capture-MS Homo sapiens
5 PLB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HS1BP3 64342
Affinity Capture-MS Homo sapiens
7 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
8 ICE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GSK3B 2932
Affinity Capture-MS Homo sapiens
10 AGPAT1 10554
Affinity Capture-MS Homo sapiens
11 ITLN2  
Affinity Capture-MS Homo sapiens
12 MAGEB4  
Affinity Capture-MS Homo sapiens
13 ALB 213
Co-fractionation Homo sapiens
14 GNG8  
Affinity Capture-MS Homo sapiens
15 TIMM10  
Affinity Capture-MS Homo sapiens
16 ERO1L 30001
Affinity Capture-MS Homo sapiens
17 HSPB1 3315
Co-fractionation Homo sapiens
18 CYP3A43  
Affinity Capture-MS Homo sapiens
19 RHBDD1 84236
Affinity Capture-MS Homo sapiens
20 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
21 USP36  
Affinity Capture-MS Homo sapiens
22 EGFL8  
Affinity Capture-MS Homo sapiens
23 FRMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TMEM257  
Affinity Capture-MS Homo sapiens
25 KLK10 5655
Affinity Capture-MS Homo sapiens
26 LRRC17 10234
Affinity Capture-MS Homo sapiens
27 S100A8 6279
Co-fractionation Homo sapiens
28 STK11 6794
Affinity Capture-MS Homo sapiens
29 PRKX 5613
Affinity Capture-MS Homo sapiens
30 Dynll1 56455
Affinity Capture-MS Mus musculus
31 SMIM8  
Affinity Capture-MS Homo sapiens
32 ELANE 1991
Co-fractionation Homo sapiens
33 FBXW8 26259
Affinity Capture-MS Homo sapiens
34 APCDD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 LTF 4057
Affinity Capture-MS Homo sapiens
36 CCR1  
Affinity Capture-MS Homo sapiens
37 CLCN2  
Affinity Capture-MS Homo sapiens
38 STX17 55014
Affinity Capture-MS Homo sapiens
39 CEBPA  
Protein-peptide Homo sapiens
40 RAD21 5885
Affinity Capture-Western Homo sapiens
41 MAEA 10296
Affinity Capture-MS Homo sapiens
42 TCEB3B  
Affinity Capture-MS Homo sapiens
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