Gene description for PRKX
Gene name protein kinase, X-linked
Gene symbol PRKX
Other names/aliases PKX1
Species Homo sapiens
 Database cross references - PRKX
ExoCarta ExoCarta_5613
Vesiclepedia VP_5613
Entrez Gene 5613
HGNC 9441
MIM 300083
UniProt P51817  
 PRKX identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for PRKX
Molecular Function
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    cAMP-dependent protein kinase activity GO:0004691 IBA
    cAMP-dependent protein kinase activity GO:0004691 IDA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    angiogenesis GO:0001525 IMP
    endothelial cell proliferation GO:0001935 IMP
    chromatin remodeling GO:0006338 IEA
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 IMP
    signal transduction GO:0007165 IBA
    peptidyl-serine phosphorylation GO:0018105 IDA
    myeloid cell differentiation GO:0030099 IDA
    regulation of cell adhesion GO:0030155 IDA
    regulation of cell migration GO:0030334 IDA
    cell-substrate adhesion GO:0031589 IMP
    endothelial cell migration GO:0043542 IMP
    protein autophosphorylation GO:0046777 IDA
    epithelial tube morphogenesis GO:0060562 IDA
    kidney morphogenesis GO:0060993 IDA
    regulation of epithelial cell differentiation involved in kidney development GO:2000696 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cAMP-dependent protein kinase complex GO:0005952 IBA
 Experiment description of studies that identified PRKX in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for PRKX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 JCHAIN 3512
Affinity Capture-MS Homo sapiens
2 SERPINA1 5265
Affinity Capture-MS Homo sapiens
3 IGLC2 3538
Affinity Capture-MS Homo sapiens
4 CASP14 23581
Affinity Capture-MS Homo sapiens
5 LYZ 4069
Affinity Capture-MS Homo sapiens
6 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
7 NEDD4 4734
Biochemical Activity Homo sapiens
8 XPO1 7514
Affinity Capture-MS Homo sapiens
9 IGHA1 3493
Affinity Capture-MS Homo sapiens
10 IGLL5 100423062
Affinity Capture-MS Homo sapiens
11 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
12 LCP1 3936
Affinity Capture-MS Homo sapiens
13 TUBB8 347688
Affinity Capture-MS Homo sapiens
14 MPO 4353
Affinity Capture-MS Homo sapiens
15 IGHA2 3494
Affinity Capture-MS Homo sapiens
16 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
17 AZGP1 563
Affinity Capture-MS Homo sapiens
18 ALOX12B  
Affinity Capture-MS Homo sapiens
19 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 APOD 347
Affinity Capture-MS Homo sapiens
21 TF 7018
Affinity Capture-MS Homo sapiens
22 HAL 3034
Affinity Capture-MS Homo sapiens
23 CTSG 1511
Affinity Capture-MS Homo sapiens
24 FKBP5 2289
Affinity Capture-MS Homo sapiens
25 IGKC 3514
Affinity Capture-MS Homo sapiens
26 VIM 7431
Affinity Capture-MS Homo sapiens
27 NEDD4L 23327
Biochemical Activity Homo sapiens
28 C3 718
Affinity Capture-MS Homo sapiens
29 LCN1 3933
Affinity Capture-MS Homo sapiens
30 ALB 213
Affinity Capture-MS Homo sapiens
31 LTF 4057
Affinity Capture-MS Homo sapiens
32 RAD54L2  
Affinity Capture-MS Homo sapiens
33 PRKAR1A 5573
Reconstituted Complex Homo sapiens
34 Prkacb 18749
Affinity Capture-MS Mus musculus
35 PIGR 5284
Affinity Capture-MS Homo sapiens
36 KPRP 448834
Affinity Capture-MS Homo sapiens
37 DMBT1 1755
Affinity Capture-MS Homo sapiens
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