Experiment description of studies that identified JCHAIN in exosomes

1 Experiment ID 494 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ EV Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 126 MISEV standards ✘ EV Biophysical techniques ✔ GAPDH EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

4 Experiment ID 44 MISEV standards ✔ IEM EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19028452     Organism Homo sapiens Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein. Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ Journal name BBRC Publication year 2009 Sample Plasma Sample name Plasma Isolation/purification methods Filtration Size exclusion Sucrose density gradient Flotation density 1.15-1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

5 Experiment ID 66 MISEV standards ✔ IEM EV Biophysical techniques ✔ TSG101|Alix|CD63|CD81 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

6 Experiment ID 217 MISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|CD81|CD9|CD63 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 238 MISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25452312     Organism Homo sapiens Experiment description N-linked (N-) glycoproteomics of urimary exosomes Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R. Journal name Mol Cell Proteomics Publication year 2014 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Filtration Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for JCHAIN

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CD79A   Invitro Homo sapiens Invitro Homo sapiens View the network

Pathways in which JCHAIN is involved

No pathways found