Gene description for JCHAIN
Gene name joining chain of multimeric IgA and IgM
Gene symbol JCHAIN
Other names/aliases IGCJ
IGJ
JCH
Species Homo sapiens
 Database cross references - JCHAIN
ExoCarta ExoCarta_3512
Vesiclepedia VP_3512
Entrez Gene 3512
HGNC 5713
MIM 147790
UniProt P01591  
 JCHAIN identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Plasma 19028452    
Saliva 19199708    
Thymus 23844026    
Urine 25452312    
 Gene ontology annotations for JCHAIN
Molecular Function
    single-stranded DNA binding GO:0003697 IDA
    antigen binding GO:0003823 NAS
    IgA binding GO:0019862 IMP
    protein-macromolecule adaptor activity GO:0030674 IEA
    phosphatidylcholine binding GO:0031210 IDA
    immunoglobulin receptor binding GO:0034987 IBA
    immunoglobulin receptor binding GO:0034987 IDA
    immunoglobulin receptor binding GO:0034987 IMP
    protein homodimerization activity GO:0042803 IDA
    peptidoglycan binding GO:0042834 IDA
Biological Process
    adaptive immune response GO:0002250 IDA
    glomerular filtration GO:0003094 IMP
    immune response GO:0006955 NAS
    humoral immune response GO:0006959 IBA
    antibacterial humoral response GO:0019731 IDA
    innate immune response GO:0045087 IDA
    positive regulation of respiratory burst GO:0060267 IDA
    protein-containing complex assembly GO:0065003 IMP
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular exosome GO:0070062 HDA
    monomeric IgA immunoglobulin complex GO:0071748 IDA
    dimeric IgA immunoglobulin complex GO:0071750 IMP
    secretory IgA immunoglobulin complex GO:0071751 IDA
    secretory dimeric IgA immunoglobulin complex GO:0071752 IDA
    pentameric IgM immunoglobulin complex GO:0071756 IBA
    pentameric IgM immunoglobulin complex GO:0071756 IDA
    hexameric IgM immunoglobulin complex GO:0071757 IDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified JCHAIN in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 238
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R."
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for JCHAIN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLEKHG6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 KLK10 5655
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 WDR6 11180
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FAM122C  
Affinity Capture-MS Homo sapiens
6 OR13C3  
Affinity Capture-MS Homo sapiens
7 ICE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HBQ1  
Affinity Capture-MS Homo sapiens
9 ARMCX3 51566
Affinity Capture-MS Homo sapiens
10 TP53 7157
Affinity Capture-MS Homo sapiens
11 HVCN1 84329
Affinity Capture-MS Homo sapiens
12 TRIAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TMEM176B 28959
Affinity Capture-MS Homo sapiens
14 PMEPA1 56937
Affinity Capture-MS Homo sapiens
15 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RUNX2  
Affinity Capture-MS Homo sapiens
17 USP36  
Affinity Capture-MS Homo sapiens
18 CCR1  
Affinity Capture-MS Homo sapiens
19 SLC25A15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 EBF2  
Affinity Capture-MS Homo sapiens
21 FOXN3 1112
Affinity Capture-MS Homo sapiens
22 NOX4  
Affinity Capture-MS Homo sapiens
23 ATP11C 286410
Affinity Capture-MS Homo sapiens
24 SNF8 11267
Affinity Capture-MS Homo sapiens
25 GTF2B 2959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 FNDC5  
Affinity Capture-MS Homo sapiens
27 FKBP14  
Affinity Capture-MS Homo sapiens
28 TIMM50 92609
Affinity Capture-MS Homo sapiens
29 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
30 COG2 22796
Affinity Capture-MS Homo sapiens
31 USB1  
Affinity Capture-MS Homo sapiens
32 EFNA3  
Affinity Capture-MS Homo sapiens
33 KIR2DS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RIBC1  
Affinity Capture-MS Homo sapiens
35 GJB5  
Affinity Capture-MS Homo sapiens
36 FRMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ZCCHC17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TMEM257  
Affinity Capture-MS Homo sapiens
39 TMEM237 65062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ZNF491  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ITLN2  
Affinity Capture-MS Homo sapiens
42 SF3B6 51639
Affinity Capture-MS Homo sapiens
43 CYP3A43  
Affinity Capture-MS Homo sapiens
44 HBM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 RHOBTB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FCN1 2219
Affinity Capture-MS Homo sapiens
47 STK11 6794
Affinity Capture-MS Homo sapiens
48 HINT2 84681
Affinity Capture-MS Homo sapiens
49 PRKX 5613
Affinity Capture-MS Homo sapiens
50 SNW1 22938
Affinity Capture-MS Homo sapiens
51 TWF1 5756
Affinity Capture-MS Homo sapiens
52 TIMM10  
Affinity Capture-MS Homo sapiens
53 APCDD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 DISP1 84976
Affinity Capture-MS Homo sapiens
55 NPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 JAK2 3717
Affinity Capture-MS Homo sapiens
57 SFXN4  
Affinity Capture-MS Homo sapiens
58 CLCN2  
Affinity Capture-MS Homo sapiens
59 GNG8  
Affinity Capture-MS Homo sapiens
60 SOX2  
Affinity Capture-MS Homo sapiens
61 TCEB3B  
Affinity Capture-MS Homo sapiens
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