Gene description for ATP11C
Gene name ATPase, class VI, type 11C
Gene symbol ATP11C
Other names/aliases ATPIG
ATPIQ
Species Homo sapiens
 Database cross references - ATP11C
ExoCarta ExoCarta_286410
Vesiclepedia VP_286410
Entrez Gene 286410
HGNC 13554
MIM 300516
UniProt Q8NB49  
 ATP11C identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ATP11C
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    phosphatidylethanolamine flippase activity GO:0090555 IDA
    phosphatidylserine floppase activity GO:0090556 IEA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 IBA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 TAS
    phosphatidylserine flippase activity GO:0140346 IDA
Biological Process
    monoatomic ion transmembrane transport GO:0034220 TAS
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 IDA
    phospholipid translocation GO:0045332 NAS
    aminophospholipid translocation GO:0140331 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    early endosome membrane GO:0031901 IEA
    recycling endosome GO:0055037 IBA
    recycling endosome membrane GO:0055038 IEA
    phospholipid-translocating ATPase complex GO:1990531 IDA
    phospholipid-translocating ATPase complex GO:1990531 IPI
 Experiment description of studies that identified ATP11C in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ATP11C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STX12 23673
Affinity Capture-MS Homo sapiens
2 SFT2D1 113402
Affinity Capture-MS Homo sapiens
3 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SLC39A12  
Affinity Capture-MS Homo sapiens
5 RPA2 6118
Proximity Label-MS Homo sapiens
6 CANX 821
Affinity Capture-MS Homo sapiens
7 JCHAIN 3512
Affinity Capture-MS Homo sapiens
8 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 LYPD3 27076
Affinity Capture-MS Homo sapiens
10 TMEM107 84314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NKAIN1  
Affinity Capture-MS Homo sapiens
12 VAMP3 9341
Affinity Capture-MS Homo sapiens
13 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
14 TMEM30A 55754
Affinity Capture-MS Homo sapiens
15 PTH1R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 Vps4b 20479
Affinity Capture-MS Mus musculus
17 TTYH1  
Affinity Capture-MS Homo sapiens
18 MBLAC2 153364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RXFP1  
Affinity Capture-MS Homo sapiens
20 SLC22A2 6582
Affinity Capture-MS Homo sapiens
21 OR10H2  
Affinity Capture-MS Homo sapiens
22 ATP11A 23250
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
23 LAMP3  
Proximity Label-MS Homo sapiens
24 IGHA1 3493
Affinity Capture-MS Homo sapiens
25 SEMA7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 GCNT3 9245
Affinity Capture-MS Homo sapiens
27 MFSD4  
Affinity Capture-MS Homo sapiens
28 ZDHHC12  
Affinity Capture-MS Homo sapiens
29 LRRC55  
Affinity Capture-MS Homo sapiens
30 TNFRSF10C  
Affinity Capture-MS Homo sapiens
31 Bmpr1a  
Affinity Capture-MS Mus musculus
32 HTR3C  
Affinity Capture-MS Homo sapiens
33 SLCO4C1 353189
Affinity Capture-MS Homo sapiens
34 IGHM 3507
Affinity Capture-MS Homo sapiens
35 SLC2A1 6513
Affinity Capture-MS Homo sapiens
36 SCGB1D1  
Affinity Capture-MS Homo sapiens
37 BTF3 689
Affinity Capture-MS Homo sapiens
38 SLC34A2 10568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 LAMP2 3920
Proximity Label-MS Homo sapiens
40 BCAM 4059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MS4A6E  
Affinity Capture-MS Homo sapiens
42 SLC17A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KCNMB3  
Affinity Capture-MS Homo sapiens
44 TMEM230 29058
Affinity Capture-MS Homo sapiens
45 SLC6A1  
Affinity Capture-MS Homo sapiens
46 MCAM 4162
Affinity Capture-MS Homo sapiens
47 GPRC5D  
Affinity Capture-MS Homo sapiens
48 IGHA2 3494
Affinity Capture-MS Homo sapiens
49 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CHRNA9  
Affinity Capture-MS Homo sapiens
51 AZGP1 563
Affinity Capture-MS Homo sapiens
52 RAB2A 5862
Proximity Label-MS Homo sapiens
53 PMAIP1  
Affinity Capture-MS Homo sapiens
54 CST4 1472
Affinity Capture-MS Homo sapiens
55 SLC43A3 29015
Affinity Capture-MS Homo sapiens
56 P2RY1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PIGR 5284
Affinity Capture-MS Homo sapiens
58 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 STS 412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TMC4  
Affinity Capture-MS Homo sapiens
61 OSBP 5007
Affinity Capture-MS Homo sapiens
62 LACRT 90070
Affinity Capture-MS Homo sapiens
63 PRR4 11272
Affinity Capture-MS Homo sapiens
64 SLC14A1  
Affinity Capture-MS Homo sapiens
65 SCN2B  
Affinity Capture-MS Homo sapiens
66 MUC1 4582
Affinity Capture-MS Homo sapiens
67 LCN1 3933
Affinity Capture-MS Homo sapiens
68 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 LTF 4057
Affinity Capture-MS Homo sapiens
70 UPK1A 11045
Affinity Capture-MS Homo sapiens
71 SIDT2 51092
Affinity Capture-MS Homo sapiens
72 MTUS2 23281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ARRDC5  
Affinity Capture-MS Homo sapiens
74 NRN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 NMUR2  
Affinity Capture-MS Homo sapiens
76 TAS1R2  
Affinity Capture-MS Homo sapiens
77 CLRN2  
Affinity Capture-MS Homo sapiens
78 ZACN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 MARCKSL1 65108
Affinity Capture-MS Homo sapiens
80 POPDC2  
Affinity Capture-MS Homo sapiens
81 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATP11C is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Transport of small molecules TAS Reactome





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