Gene description for LCN1
Gene name lipocalin 1
Gene symbol LCN1
Other names/aliases PMFA
TLC
TP
VEGP
Species Homo sapiens
 Database cross references - LCN1
ExoCarta ExoCarta_3933
Vesiclepedia VP_3933
Entrez Gene 3933
HGNC 6525
MIM 151675
UniProt P31025  
 LCN1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for LCN1
Molecular Function
    cysteine-type endopeptidase inhibitor activity GO:0004869 TAS
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    chloride ion binding GO:0031404 IDA
Biological Process
    proteolysis GO:0006508 TAS
    sensory perception of taste GO:0050909 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified LCN1 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LCN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WDFY3 23001
Affinity Capture-MS Homo sapiens
2 PMEPA1 56937
Affinity Capture-MS Homo sapiens
3 FCN1 2219
Affinity Capture-MS Homo sapiens
4 SERPINA1 5265
Affinity Capture-MS Homo sapiens
5 MRPL42  
Affinity Capture-MS Homo sapiens
6 HBB 3043
Affinity Capture-MS Homo sapiens
7 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ITGA5 3678
Affinity Capture-MS Homo sapiens
9 ALKBH2  
Affinity Capture-MS Homo sapiens
10 TF 7018
Affinity Capture-MS Homo sapiens
11 STATH  
Affinity Capture-MS Homo sapiens
12 DTX3L 151636
Affinity Capture-MS Homo sapiens
13 SPRY2 10253
Affinity Capture-MS Homo sapiens
14 FANCD2  
Affinity Capture-MS Homo sapiens
15 WHSC1 7468
Affinity Capture-MS Homo sapiens
16 TDRKH  
Affinity Capture-MS Homo sapiens
17 KDELR3 11015
Affinity Capture-MS Homo sapiens
18 UBE3A 7337
Affinity Capture-MS Homo sapiens
19 PCCB 5096
Affinity Capture-MS Homo sapiens
20 WWOX 51741
Affinity Capture-MS Homo sapiens
21 CADPS  
Affinity Capture-MS Homo sapiens
22 DDX3X 1654
Affinity Capture-MS Homo sapiens
23 TMEM176B 28959
Affinity Capture-MS Homo sapiens
24 UBQLN2 29978
Two-hybrid Homo sapiens
25 GC 2638
Affinity Capture-MS Homo sapiens
26 EGFL8  
Affinity Capture-MS Homo sapiens
27 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PDYN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PLSCR4  
Affinity Capture-MS Homo sapiens
30 CAPSL  
Affinity Capture-MS Homo sapiens
31 FAM25A  
Two-hybrid Homo sapiens
32 MYL3 4634
Affinity Capture-MS Homo sapiens
33 KHK 3795
Affinity Capture-MS Homo sapiens
34 RPSAP52  
Affinity Capture-MS Homo sapiens
35 CP 1356
Affinity Capture-MS Homo sapiens
36 SFXN4  
Affinity Capture-MS Homo sapiens
37 TMEM116  
Affinity Capture-MS Homo sapiens
38 CCDC94  
Affinity Capture-MS Homo sapiens
39 SERPINA3 12
Affinity Capture-MS Homo sapiens
40 SPNS3  
Affinity Capture-MS Homo sapiens
41 AZGP1 563
Affinity Capture-MS Homo sapiens
42 CRAMP1L 57585
Affinity Capture-MS Homo sapiens
43 FKBP14  
Affinity Capture-MS Homo sapiens
44 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
45 HPX 3263
Affinity Capture-MS Homo sapiens
46 KLHL22 84861
Affinity Capture-MS Homo sapiens
47 DNAJB8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ARMC6 93436
Affinity Capture-MS Homo sapiens
49 FAM25G  
Two-hybrid Homo sapiens
50 HP 3240
Affinity Capture-MS Homo sapiens
51 PECR 55825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 GOLM1 51280
Affinity Capture-MS Homo sapiens
53 RNMTL1  
Affinity Capture-MS Homo sapiens
54 ITLN2  
Affinity Capture-MS Homo sapiens
55 EFNA3  
Affinity Capture-MS Homo sapiens
56 ORM1 5004
Affinity Capture-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 PINK1  
Affinity Capture-MS Homo sapiens
59 FAM25C  
Two-hybrid Homo sapiens
60 HBM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KLK10 5655
Affinity Capture-MS Homo sapiens
62 FOXN3 1112
Affinity Capture-MS Homo sapiens
63 HYOU1 10525
Affinity Capture-MS Homo sapiens
64 LRRC8E 80131
Affinity Capture-MS Homo sapiens
65 MRPS5 64969
Affinity Capture-MS Homo sapiens
66 PRR4 11272
Affinity Capture-MS Homo sapiens
67 RECK 8434
Two-hybrid Homo sapiens
68 ATAT1  
Affinity Capture-MS Homo sapiens
69 PRKX 5613
Affinity Capture-MS Homo sapiens
70 B3GNT2 10678
Affinity Capture-MS Homo sapiens
71 Cdca5  
Affinity Capture-MS Mus musculus
72 SPATA4  
Affinity Capture-MS Homo sapiens
73 APOA1 335
Affinity Capture-MS Homo sapiens
74 ATP11C 286410
Affinity Capture-MS Homo sapiens
75 NKAP 79576
Affinity Capture-MS Homo sapiens
76 NPHP4 261734
Affinity Capture-MS Homo sapiens
77 IGLC1 3537
Affinity Capture-MS Homo sapiens
78 LMBR1L  
Reconstituted Complex Homo sapiens
79 GJB5  
Affinity Capture-MS Homo sapiens
80 CYLD  
Affinity Capture-MS Homo sapiens
81 CUL4A 8451
Affinity Capture-MS Homo sapiens
82 DARS2 55157
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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