Gene description for MYL3
Gene name myosin, light chain 3, alkali; ventricular, skeletal, slow
Gene symbol MYL3
Other names/aliases CMH8
MLC1SB
MLC1V
VLC1
Species Homo sapiens
 Database cross references - MYL3
ExoCarta ExoCarta_4634
Vesiclepedia VP_4634
Entrez Gene 4634
HGNC 7584
MIM 160790
UniProt P08590  
 MYL3 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MYL3
Molecular Function
    actin monomer binding GO:0003785 IDA
    calcium ion binding GO:0005509 IEA
    structural constituent of muscle GO:0008307 TAS
    myosin II heavy chain binding GO:0032038 NAS
Biological Process
    regulation of the force of heart contraction GO:0002026 IMP
    regulation of striated muscle contraction GO:0006942 IMP
    positive regulation of ATP-dependent activity GO:0032781 ISS
    ventricular cardiac muscle tissue morphogenesis GO:0055010 IMP
    cardiac muscle contraction GO:0060048 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 TAS
    myosin II complex GO:0016460 IBA
    sarcomere GO:0030017 TAS
    A band GO:0031672 IDA
    I band GO:0031674 IDA
 Experiment description of studies that identified MYL3 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SP1  
Affinity Capture-MS Homo sapiens
2 YWHAQ 10971
Affinity Capture-MS Homo sapiens
3 IQGAP1 8826
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MYH14 79784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MYH10 4628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MYO18A 399687
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 UBC 7316
Reconstituted Complex Homo sapiens
8 SIK2  
Affinity Capture-MS Homo sapiens
9 MECOM 2122
Affinity Capture-MS Homo sapiens
10 LCN1 3933
Affinity Capture-MS Homo sapiens
11 ATG101  
Two-hybrid Homo sapiens
12 MYH9 4627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TAF6 6878
Cross-Linking-MS (XL-MS) Homo sapiens
14 MYL4 4635
Affinity Capture-MS Homo sapiens
15 CUL4A 8451
Affinity Capture-MS Homo sapiens
16 FTH1 2495
Two-hybrid Homo sapiens
17 MYC  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MYL3 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





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