Gene description for MYL3
Gene name myosin, light chain 3, alkali; ventricular, skeletal, slow
Gene symbol MYL3
Other names/aliases CMH8
MLC1SB
MLC1V
VLC1
Species Homo sapiens
 Database cross references - MYL3
ExoCarta ExoCarta_4634
Entrez Gene 4634
HGNC 7584
MIM 160790
UniProt P08590  
 MYL3 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MYL3
Molecular Function
    calcium ion binding GO:0005509 IEA
    structural constituent of muscle GO:0008307 TAS
    actin monomer binding GO:0003785 IDA
    myosin II heavy chain binding GO:0032038 NAS
    motor activity GO:0003774 IEA
Biological Process
    skeletal muscle tissue development GO:0007519 IEA
    cardiac muscle contraction GO:0060048 IMP
    metabolic process GO:0008152 IEA
    muscle filament sliding GO:0030049 TAS
    regulation of the force of heart contraction GO:0002026 IMP
    ventricular cardiac muscle tissue morphogenesis GO:0055010 IMP
    positive regulation of ATPase activity GO:0032781 ISS
    regulation of striated muscle contraction GO:0006942 IMP
Subcellular Localization
    I band GO:0031674 IDA
    sarcomere GO:0030017 TAS
    A band GO:0031672 IDA
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 TAS
 Experiment description of studies that identified MYL3 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MYL3 is involved
PathwayEvidenceSource
Striated Muscle Contraction TAS Reactome





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