Gene description for IQGAP1
Gene name IQ motif containing GTPase activating protein 1
Gene symbol IQGAP1
Other names/aliases HUMORFA01
SAR1
p195
Species Homo sapiens
 Database cross references - IQGAP1
ExoCarta ExoCarta_8826
Vesiclepedia VP_8826
Entrez Gene 8826
HGNC 6110
MIM 603379
UniProt P46940  
 IQGAP1 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast milk 17641064    
Breast milk 17641064    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Endothelial cells 26027894    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for IQGAP1
Molecular Function
    MAP-kinase scaffold activity GO:0005078 IPI
    GTPase inhibitor activity GO:0005095 TAS
    GTPase activator activity GO:0005096 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    protein kinase binding GO:0019901 IPI
    protein phosphatase binding GO:0019903 IPI
    protein domain specific binding GO:0019904 IPI
    small GTPase binding GO:0031267 IPI
    protein serine/threonine kinase activator activity GO:0043539 IDA
    S100 protein binding GO:0044548 IPI
    cadherin binding GO:0045296 HDA
    actin filament binding GO:0051015 IBA
    molecular adaptor activity GO:0060090 IDA
Biological Process
    MAPK cascade GO:0000165 IEA
    regulation of cytokine production GO:0001817 IEA
    signal transduction GO:0007165 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IMP
    regulation of mitotic cell cycle GO:0007346 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    fibroblast migration GO:0010761 IBA
    cell migration GO:0016477 IMP
    negative regulation of dephosphorylation GO:0035305 IEA
    cellular response to platelet-derived growth factor stimulus GO:0036120 IEA
    positive regulation of MAPK cascade GO:0043410 IMP
    positive regulation of protein kinase activity GO:0045860 IMP
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    caveola assembly GO:0070836 IEA
    cellular response to calcium ion GO:0071277 IDA
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    podocyte development GO:0072015 ISS
    mitotic actomyosin contractile ring assembly actin filament organization GO:1903479 IBA
    neuron projection extension GO:1990138 IMP
Subcellular Localization
    ruffle GO:0001726 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    actin filament GO:0005884 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    cell cortex GO:0005938 IBA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    actin cytoskeleton GO:0015629 ISS
    microtubule cytoskeleton GO:0015630 ISS
    basolateral plasma membrane GO:0016323 IEA
    apical plasma membrane GO:0016324 IDA
    lateral plasma membrane GO:0016328 IEA
    axon GO:0030424 ISS
    growth cone GO:0030426 ISS
    midbody GO:0030496 IDA
    secretory granule membrane GO:0030667 TAS
    cortical actin cytoskeleton GO:0030864 IEA
    slit diaphragm GO:0036057 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    neuron projection GO:0043005 ISS
    extracellular exosome GO:0070062 HDA
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified IQGAP1 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
13
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
14
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
19
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
20
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
21
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
22
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
23
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
24
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
25
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
31
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
40
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
41
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
42
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
44
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
45
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
46
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
47
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
48
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
49
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
52
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
53
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
54
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
55
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
56
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
57
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
58
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
59
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
60
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
61
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
62
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
63
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
64
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
65
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
66
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
67
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
68
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IQGAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ISG15 9636
Affinity Capture-MS Homo sapiens
2 Cdk1 12534
Affinity Capture-MS Mus musculus
3 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HDAC2 3066
Co-fractionation Homo sapiens
5 SHC1 6464
Affinity Capture-MS Homo sapiens
6 ARPC2 10109
Affinity Capture-MS Homo sapiens
7 DYNC1I2 1781
Co-fractionation Homo sapiens
8 TPM2 7169
Affinity Capture-MS Homo sapiens
9 ARPC4 10093
Affinity Capture-MS Homo sapiens
10 Calml3  
Affinity Capture-MS Mus musculus
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 CKB 1152
Co-fractionation Homo sapiens
15 HIST1H1A 3024
Co-fractionation Homo sapiens
16 DTX3  
Affinity Capture-MS Homo sapiens
17 KIF23 9493
Affinity Capture-MS Homo sapiens
18 ACTR3 10096
Affinity Capture-MS Homo sapiens
19 AP2A1 160
Affinity Capture-MS Homo sapiens
20 TRIOBP 11078
Affinity Capture-MS Homo sapiens
21 PPP1R18 170954
Affinity Capture-MS Homo sapiens
22 SRC 6714
Affinity Capture-Western Homo sapiens
23 PSMB6 5694
Co-fractionation Homo sapiens
24 TFIP11  
Affinity Capture-MS Homo sapiens
25 SMC2 10592
Co-fractionation Homo sapiens
26 GSPT1 2935
Co-fractionation Homo sapiens
27 ILF3 3609
Co-fractionation Homo sapiens
28 GTSE1 51512
Affinity Capture-MS Homo sapiens
29 PPP6R3 55291
Co-fractionation Homo sapiens
30 GPS1 2873
Co-fractionation Homo sapiens
31 WDR5 11091
Co-fractionation Homo sapiens
32 SOD1 6647
Affinity Capture-MS Homo sapiens
33 TES 26136
Affinity Capture-MS Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 ACIN1 22985
Affinity Capture-MS Homo sapiens
36 SRSF6 6431
Affinity Capture-MS Homo sapiens
37 GOLIM4 27333
Affinity Capture-MS Homo sapiens
38 CHMP4C 92421
Affinity Capture-MS Homo sapiens
39 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
40 SORBS2  
Affinity Capture-MS Homo sapiens
41 LUZP1 7798
Affinity Capture-MS Homo sapiens
42 MCAM 4162
Proximity Label-MS Homo sapiens
43 CAMK2G 818
Affinity Capture-MS Homo sapiens
44 UBASH3B 84959
Affinity Capture-MS Homo sapiens
45 PABPC1 26986
Co-fractionation Homo sapiens
46 CDH1 999
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
47 LRRFIP1 9208
Affinity Capture-MS Homo sapiens
48 YAP1 10413
Affinity Capture-MS Homo sapiens
49 MYH9 4627
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
50 ACTN4 81
Affinity Capture-MS Homo sapiens
51 ARHGAP11A  
Affinity Capture-MS Homo sapiens
52 SYNCRIP 10492
Co-fractionation Homo sapiens
53 PEX14 5195
Co-purification Homo sapiens
54 XPOT 11260
Co-fractionation Homo sapiens
55 YWHAQ 10971
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
56 Mapk13  
Affinity Capture-MS Mus musculus
57 FNBP1 23048
Co-fractionation Homo sapiens
58 HECTD1 25831
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 DDX39B 7919
Affinity Capture-MS Homo sapiens
60 VDAC2 7417
Co-fractionation Homo sapiens
61 SRPK1 6732
Affinity Capture-MS Homo sapiens
62 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
63 ANLN 54443
Affinity Capture-MS Homo sapiens
64 DST 667
Affinity Capture-MS Homo sapiens
65 HSPA5 3309
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
66 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
67 MYL3 4634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SPECC1 92521
Affinity Capture-MS Homo sapiens
69 LMNB1 4001
Co-fractionation Homo sapiens
70 YBX1 4904
Affinity Capture-MS Homo sapiens
71 SIRT7  
Affinity Capture-MS Homo sapiens
72 LMO7 4008
Affinity Capture-MS Homo sapiens
73 CUL3 8452
Affinity Capture-MS Homo sapiens
74 CYP1A1  
Affinity Capture-MS Homo sapiens
75 MYO19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 LRRC47 57470
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
77 FN1 2335
Affinity Capture-MS Homo sapiens
78 CDC42 998
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 UBA2 10054
Co-fractionation Homo sapiens
80 TJP2 9414
Affinity Capture-MS Homo sapiens
81 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
82 DLST 1743
Co-fractionation Homo sapiens
83 AP2A2 161
Affinity Capture-MS Homo sapiens
84 PSMC5 5705
Co-fractionation Homo sapiens
85 AKT1 207
Affinity Capture-Western Homo sapiens
86 NFATC2  
Affinity Capture-Western Homo sapiens
87 TPM4 7171
Affinity Capture-MS Homo sapiens
88 CEPT1 10390
Affinity Capture-MS Homo sapiens
89 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 ARPC3 10094
Affinity Capture-MS Homo sapiens
91 USP46 64854
Affinity Capture-MS Homo sapiens
92 DAB2 1601
Affinity Capture-MS Homo sapiens
93 TGFBR2 7048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 SSFA2 6744
Affinity Capture-MS Homo sapiens
95 PLEC 5339
Affinity Capture-MS Homo sapiens
96 RUVBL2 10856
Co-fractionation Homo sapiens
97 MISP 126353
Affinity Capture-MS Homo sapiens
98 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
99 CDK15  
Affinity Capture-MS Homo sapiens
100 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
101 MYH1  
Affinity Capture-MS Homo sapiens
102 PSMA1 5682
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
103 DYNC1H1 1778
Co-fractionation Homo sapiens
104 RAB2A 5862
Proximity Label-MS Homo sapiens
105 Cobll1 319876
Affinity Capture-MS Mus musculus
106 PSMA5 5686
Co-fractionation Homo sapiens
107 CDK4 1019
Affinity Capture-MS Homo sapiens
108 DAPK3 1613
Affinity Capture-MS Homo sapiens
109 CLTA 1211
Affinity Capture-MS Homo sapiens
110 RAC3 5881
Affinity Capture-MS Homo sapiens
111 SNW1 22938
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 ADRM1 11047
Co-fractionation Homo sapiens
113 ANXA2 302
Affinity Capture-MS Homo sapiens
114 GAS2L1 10634
Affinity Capture-MS Homo sapiens
115 PPARGC1A  
Affinity Capture-MS Homo sapiens
116 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 TRPC4AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 PSMD3 5709
Co-fractionation Homo sapiens
119 SMC3 9126
Affinity Capture-MS Homo sapiens
120 ABLIM1 3983
Affinity Capture-MS Homo sapiens
121 MCM2 4171
Affinity Capture-MS Homo sapiens
122 WDR3  
Co-fractionation Homo sapiens
123 EIF2S3 1968
Co-fractionation Homo sapiens
124 RPA2 6118
Affinity Capture-MS Homo sapiens
125 DBN1 1627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
127 CLTB 1212
Affinity Capture-MS Homo sapiens
128 CORO1B 57175
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
129 DIAPH1 1729
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
130 MYO5B 4645
Affinity Capture-MS Homo sapiens
131 PRC1 9055
Affinity Capture-MS Homo sapiens
132 SRRM2 23524
Affinity Capture-MS Homo sapiens
133 PARVA 55742
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 MAPK14 1432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 OBSL1 23363
Affinity Capture-MS Homo sapiens
136 TPRN 286262
Affinity Capture-MS Homo sapiens
137 VCAM1 7412
Affinity Capture-MS Homo sapiens
138 USP7 7874
Co-fractionation Homo sapiens
139 TWF1 5756
Affinity Capture-MS Homo sapiens
140 PSMB3 5691
Co-fractionation Homo sapiens
141 PRNP 5621
Affinity Capture-MS Homo sapiens
142 KDR  
Affinity Capture-Western Homo sapiens
143 YWHAB 7529
Cross-Linking-MS (XL-MS) Homo sapiens
144 CCT2 10576
Co-fractionation Homo sapiens
145 COBL  
Affinity Capture-MS Homo sapiens
146 CALM2 805
Affinity Capture-MS Homo sapiens
147 MROH6  
Affinity Capture-MS Homo sapiens
148 MAPK3 5595
Co-purification Homo sapiens
149 DSG1 1828
Affinity Capture-MS Homo sapiens
150 GGPS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 WHSC1 7468
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
152 RAI14 26064
Affinity Capture-MS Homo sapiens
153 LYN 4067
Proximity Label-MS Homo sapiens
154 CBL 867
Affinity Capture-MS Homo sapiens
155 RCC2 55920
Affinity Capture-MS Homo sapiens
156 SNRPD1 6632
Co-fractionation Homo sapiens
157 RAP1A 5906
Affinity Capture-MS Homo sapiens
158 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
159 LUC7L2 51631
Co-fractionation Homo sapiens
160 ALYREF 10189
Affinity Capture-MS Homo sapiens
161 KLC1 3831
Co-fractionation Homo sapiens
162 GOT1 2805
Affinity Capture-MS Homo sapiens
163 HNRNPL 3191
Co-fractionation Homo sapiens
164 RPLP0 6175
Co-fractionation Homo sapiens
165 KCTD3  
Affinity Capture-MS Homo sapiens
166 AIMP2 7965
Co-fractionation Homo sapiens
167 PTGR2 145482
Affinity Capture-MS Homo sapiens
168 EAF1  
Affinity Capture-MS Homo sapiens
169 USP15 9958
Co-fractionation Homo sapiens
170 PKNOX2  
Two-hybrid Homo sapiens
171 UBE2L6 9246
Affinity Capture-MS Homo sapiens
172 EGFR 1956
Affinity Capture-Western Homo sapiens
173 CCT3 7203
Cross-Linking-MS (XL-MS) Homo sapiens
174 PACSIN3 29763
Affinity Capture-MS Homo sapiens
175 KBTBD7  
Affinity Capture-MS Homo sapiens
176 SPTBN1 6711
Affinity Capture-MS Homo sapiens
177 CDK2 1017
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
178 TPM1 7168
Affinity Capture-MS Homo sapiens
179 CORO2A  
Affinity Capture-MS Homo sapiens
180 PTK2 5747
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
181 MYC  
Affinity Capture-MS Homo sapiens
182 MYL4 4635
Affinity Capture-MS Homo sapiens
183 TMOD3 29766
Affinity Capture-MS Homo sapiens
184 DDB1 1642
Co-fractionation Homo sapiens
185 CUL7 9820
Affinity Capture-MS Homo sapiens
186 AKAP2 11217
Affinity Capture-MS Homo sapiens
187 FYTTD1  
Affinity Capture-MS Homo sapiens
188 MPRIP 23164
Affinity Capture-MS Homo sapiens
189 ENTHD2  
Two-hybrid Homo sapiens
190 FHL2 2274
Co-fractionation Homo sapiens
191 STX17 55014
Affinity Capture-MS Homo sapiens
192 HUWE1 10075
Affinity Capture-MS Homo sapiens
193 AIMP1 9255
Co-fractionation Homo sapiens
194 TAF6 6878
Co-fractionation Homo sapiens
195 MAP2K2 5605
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
196 MYL6 4637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
197 HDGF 3068
Co-fractionation Homo sapiens
198 TP53 7157
Affinity Capture-MS Homo sapiens
199 NMI  
Co-fractionation Homo sapiens
200 SIPA1 6494
Affinity Capture-MS Homo sapiens
201 EZR 7430
Reconstituted Complex Homo sapiens
202 SIPA1L3  
Affinity Capture-MS Homo sapiens
203 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
204 C1orf35  
Affinity Capture-MS Homo sapiens
205 PPP2R2C 5522
Affinity Capture-MS Homo sapiens
206 MAP2K1 5604
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
207 CDK1 983
Biochemical Activity Homo sapiens
208 DDX39A 10212
Co-fractionation Homo sapiens
209 C16orf72 29035
Affinity Capture-MS Homo sapiens
210 GAPVD1 26130
Co-fractionation Homo sapiens
211 ANXA5 308
Co-fractionation Homo sapiens
212 TNNC2  
Affinity Capture-MS Homo sapiens
213 MYL10 93408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
214 C1QBP 708
Co-fractionation Homo sapiens
215 RPS6KC1  
Affinity Capture-MS Homo sapiens
216 ATP5C1 509
Cross-Linking-MS (XL-MS) Homo sapiens
217 NRD1 4898
Affinity Capture-MS Homo sapiens
218 SSH2  
Affinity Capture-MS Homo sapiens
219 MYO18A 399687
Affinity Capture-MS Homo sapiens
220 MYL12B 103910
Affinity Capture-MS Homo sapiens
221 ZDHHC5 25921
Affinity Capture-MS Homo sapiens
222 MARCKS 4082
Proximity Label-MS Homo sapiens
223 YWHAE 7531
Co-fractionation Homo sapiens
224 LIMCH1 22998
Affinity Capture-MS Homo sapiens
225 EPS15 2060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
226 MYO5C 55930
Affinity Capture-MS Homo sapiens
227 VAT1 10493
Co-fractionation Homo sapiens
228 PSMD10 5716
Co-fractionation Homo sapiens
229 RPS6KB2  
Affinity Capture-MS Homo sapiens
230 LRPPRC 10128
Co-fractionation Homo sapiens
231 CAPZB 832
Affinity Capture-MS Homo sapiens
232 RUVBL1 8607
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
233 PINK1  
Affinity Capture-MS Homo sapiens
234 FTO 79068
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
235 VARS 7407
Co-fractionation Homo sapiens
236 SDHB 6390
Co-fractionation Homo sapiens
237 SPAG9 9043
Co-fractionation Homo sapiens
238 CLIP1 6249
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
239 RHOB 388
Proximity Label-MS Homo sapiens
240 ITGA4 3676
Affinity Capture-MS Homo sapiens
241 GOLGA4  
Co-fractionation Homo sapiens
242 ARHGEF17 9828
Affinity Capture-MS Homo sapiens
243 PSMD6 9861
Co-fractionation Homo sapiens
244 CSNK2B 1460
Affinity Capture-MS Homo sapiens
245 UQCRQ 27089
Co-fractionation Homo sapiens
246 CYBRD1 79901
Affinity Capture-MS Homo sapiens
247 HSPA8 3312
Co-fractionation Homo sapiens
248 S100A2 6273
Affinity Capture-MS Homo sapiens
249 PSMB4 5692
Co-fractionation Homo sapiens
250 PARK2  
Affinity Capture-MS Homo sapiens
251 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
252 KRAS 3845
Proximity Label-MS Homo sapiens
253 CHMP4B 128866
Affinity Capture-MS Homo sapiens
254 NUMB 8650
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
255 NTRK1 4914
Affinity Capture-MS Homo sapiens
256 U2AF2 11338
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
257 DNAJA1 3301
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
258 EEF1E1 9521
Co-fractionation Homo sapiens
259 CCDC8  
Affinity Capture-MS Homo sapiens
260 S100B 6285
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
261 ACTB 60
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
262 TUBA1B 10376
Co-fractionation Homo sapiens
263 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
264 C1orf131  
Affinity Capture-MS Homo sapiens
265 OXR1  
Affinity Capture-MS Homo sapiens
266 ACTG1 71
Affinity Capture-MS Homo sapiens
267 BZW1 9689
Co-fractionation Homo sapiens
268 ECH1 1891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
269 METTL14  
Affinity Capture-MS Homo sapiens
270 MEI4  
Two-hybrid Homo sapiens
271 ECT2 1894
Affinity Capture-MS Homo sapiens
272 DHX15 1665
Affinity Capture-MS Homo sapiens
273 HOXB5  
Affinity Capture-MS Homo sapiens
274 RPL14 9045
Affinity Capture-MS Homo sapiens
275 PSMA2 5683
Co-fractionation Homo sapiens
276 MYO5A 4644
Affinity Capture-MS Homo sapiens
277 HNRNPA1 3178
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
278 CORO1C 23603
Affinity Capture-MS Homo sapiens
279 BASP1 10409
Affinity Capture-MS Homo sapiens
280 AP2M1 1173
Affinity Capture-MS Homo sapiens
281 PDCD4 27250
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
282 MEOX2  
Two-hybrid Homo sapiens
283 SYNPO 11346
Affinity Capture-MS Homo sapiens
284 FLNB 2317
Affinity Capture-MS Homo sapiens
285 SEC16A 9919
Affinity Capture-MS Homo sapiens
286 RPS14 6208
Affinity Capture-MS Homo sapiens
287 CFL2 1073
Affinity Capture-MS Homo sapiens
288 CGN  
Affinity Capture-MS Homo sapiens
289 FLII 2314
Affinity Capture-MS Homo sapiens
290 RNF2  
Affinity Capture-MS Homo sapiens
291 SIK2  
Affinity Capture-MS Homo sapiens
292 FLOT1 10211
Proximity Label-MS Homo sapiens
293 NCAPG 64151
Co-fractionation Homo sapiens
294 TPM3 7170
Affinity Capture-MS Homo sapiens
295 HIF1A 3091
Affinity Capture-MS Homo sapiens
296 CSE1L 1434
Co-fractionation Homo sapiens
297 AHSA1 10598
Affinity Capture-Western Homo sapiens
298 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
299 SPTBN2 6712
Affinity Capture-MS Homo sapiens
300 DDX3X 1654
Affinity Capture-MS Homo sapiens
301 RPA1 6117
Affinity Capture-MS Homo sapiens
302 RPTOR 57521
Affinity Capture-Western Homo sapiens
303 EFNB1 1947
Affinity Capture-MS Homo sapiens
304 THRAP3 9967
Co-fractionation Homo sapiens
305 CREBBP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
306 C11orf52 91894
Proximity Label-MS Homo sapiens
307 TRAP1 10131
Co-fractionation Homo sapiens
308 VPS18 57617
Affinity Capture-MS Homo sapiens
309 DNM1L 10059
Affinity Capture-MS Homo sapiens
310 LBX1  
Two-hybrid Homo sapiens
311 AFAP1 60312
Affinity Capture-MS Homo sapiens
312 API5 8539
Co-fractionation Homo sapiens
313 INF2 64423
Affinity Capture-MS Homo sapiens
314 LRRK2 120892
Affinity Capture-Western Homo sapiens
315 FLNA 2316
Affinity Capture-MS Homo sapiens
316 MYO1E 4643
Affinity Capture-MS Homo sapiens
317 HNRNPF 3185
Co-fractionation Homo sapiens
318 SERBP1 26135
Affinity Capture-MS Homo sapiens
319 EID1  
Affinity Capture-MS Homo sapiens
320 CD44 960
Affinity Capture-Western Homo sapiens
321 RAC1 5879
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
322 LTF 4057
Cross-Linking-MS (XL-MS) Homo sapiens
323 ATXN3 4287
Affinity Capture-MS Homo sapiens
324 VIM 7431
Co-fractionation Homo sapiens
325 CDKN2A 1029
Affinity Capture-MS Homo sapiens
326 OTX2  
Two-hybrid Homo sapiens
327 UBE2H 7328
Affinity Capture-MS Homo sapiens
328 ACTR2 10097
Affinity Capture-MS Homo sapiens
329 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
330 LGR4 55366
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
331 QARS 5859
Co-fractionation Homo sapiens
332 Tpm1 22003
Affinity Capture-MS Mus musculus
333 ARPC5L 81873
Affinity Capture-MS Homo sapiens
334 PTBP1 5725
Co-fractionation Homo sapiens
335 PPIE 10450
Affinity Capture-MS Homo sapiens
336 FBXO25  
Affinity Capture-MS Homo sapiens
337 PBK  
Co-fractionation Homo sapiens
338 TSG101 7251
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
339 PRPSAP2 5636
Co-fractionation Homo sapiens
340 CALM1 801
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
341 TROVE2 6738
Co-fractionation Homo sapiens
342 CFL1 1072
Affinity Capture-MS Homo sapiens
343 SNRPD2 6633
Co-fractionation Homo sapiens
344 ILF2 3608
Two-hybrid Homo sapiens
345 PPP1CC 5501
Affinity Capture-MS Homo sapiens
346 KIAA1429 25962
Affinity Capture-MS Homo sapiens
347 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
348 LRP6 4040
Affinity Capture-Western Homo sapiens
349 SPECC1L 23384
Affinity Capture-MS Homo sapiens
350 KIF5B 3799
Co-fractionation Homo sapiens
351 CALM3 808
Affinity Capture-MS Homo sapiens
352 ADD1 118
Affinity Capture-MS Homo sapiens
353 YWHAG 7532
Co-fractionation Homo sapiens
354 ABCE1 6059
Affinity Capture-MS Homo sapiens
355 MYO1B 4430
Affinity Capture-MS Homo sapiens
356 WASL 8976
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
357 HNRNPK 3190
Co-fractionation Homo sapiens
358 CEP57  
Affinity Capture-MS Homo sapiens
359 RAB35 11021
Proximity Label-MS Homo sapiens
360 Lima1  
Affinity Capture-MS Mus musculus
361 LAMTOR1 55004
Proximity Label-MS Homo sapiens
362 WDR1 9948
Affinity Capture-MS Homo sapiens
363 DDX19B 11269
Affinity Capture-MS Homo sapiens
364 PPP1CA 5499
Affinity Capture-MS Homo sapiens
365 RBBP7 5931
Co-fractionation Homo sapiens
366 MRPS26 64949
Affinity Capture-MS Homo sapiens
367 CCAR2 57805
Co-fractionation Homo sapiens
368 TMOD1  
Affinity Capture-MS Homo sapiens
369 STAU1 6780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
370 Smc3 13006
Affinity Capture-MS Mus musculus
371 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
372 UBE2I 7329
Biochemical Activity Homo sapiens
373 VCP 7415
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
374 BMP2K 55589
Affinity Capture-MS Homo sapiens
375 MYH10 4628
Co-fractionation Homo sapiens
376 BRAF  
Affinity Capture-MS Homo sapiens
377 CEP76  
Affinity Capture-MS Homo sapiens
378 RAD54L2  
Cross-Linking-MS (XL-MS) Homo sapiens
379 RABIF  
Affinity Capture-MS Homo sapiens
380 CEBPA  
Protein-peptide Homo sapiens
381 YWHAH 7533
Affinity Capture-MS Homo sapiens
382 CALML3 810
Affinity Capture-MS Homo sapiens
383 LIMA1 51474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
384 ACTN1 87
Affinity Capture-MS Homo sapiens
385 ASCC3 10973
Affinity Capture-MS Homo sapiens
386 S100P 6286
Affinity Capture-MS Homo sapiens
387 CLINT1 9685
Affinity Capture-MS Homo sapiens
388 CDK9 1025
Affinity Capture-MS Homo sapiens
389 NDUFA9 4704
Co-fractionation Homo sapiens
390 EIF4G1 1981
Co-fractionation Homo sapiens
391 LAMP1 3916
Proximity Label-MS Homo sapiens
392 MYL1 4632
Protein-peptide Homo sapiens
393 CYBB 1536
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
394 ERCC8  
Affinity Capture-MS Homo sapiens
395 CTTN 2017
Affinity Capture-MS Homo sapiens
396 ARF6 382
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
397 NXF1 10482
Affinity Capture-RNA Homo sapiens
398 LZTS2 84445
Affinity Capture-MS Homo sapiens
399 PRKACA 5566
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
400 MYL6B 140465
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
401 NIN 51199
Proximity Label-MS Homo sapiens
402 AKAP5  
Reconstituted Complex Homo sapiens
403 MAGEA4 4103
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
404 CLTC 1213
Affinity Capture-MS Homo sapiens
405 PLEKHG3 26030
Affinity Capture-MS Homo sapiens
406 PPME1 51400
Affinity Capture-MS Homo sapiens
407 GSN 2934
Affinity Capture-MS Homo sapiens
408 DVL2 1856
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
409 MYO1C 4641
Affinity Capture-MS Homo sapiens
410 PDLIM7 9260
Two-hybrid Homo sapiens
411 NEXN 91624
Affinity Capture-MS Homo sapiens
412 SPTAN1 6709
Affinity Capture-MS Homo sapiens
413 CLIC1 1192
Affinity Capture-MS Homo sapiens
414 PSMD1 5707
Co-fractionation Homo sapiens
415 SVIL 6840
Affinity Capture-MS Homo sapiens
416 INPPL1 3636
Affinity Capture-MS Homo sapiens
417 SAP18 10284
Affinity Capture-MS Homo sapiens
418 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
419 MAPK1 5594
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
420 PARD6B 84612
Co-fractionation Homo sapiens
421 TRIP4 9325
Affinity Capture-MS Homo sapiens
422 PXN 5829
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
423 FBP1 2203
Affinity Capture-MS Homo sapiens
424 NAP1L5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
425 ZBTB20 26137
Affinity Capture-MS Homo sapiens
426 PIK3R2 5296
Affinity Capture-MS Homo sapiens
427 HNRNPU 3192
Co-fractionation Homo sapiens
428 CDC5L 988
Affinity Capture-MS Homo sapiens
429 DSG2 1829
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which IQGAP1 is involved
PathwayEvidenceSource
CDC42 GTPase cycle TAS Reactome
Cell-Cell communication TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Integration of energy metabolism IEA Reactome
MAP2K and MAPK activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Metabolism IEA Reactome
Nephrin family interactions TAS Reactome
Neutrophil degranulation TAS Reactome
Oncogenic MAPK signaling TAS Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
RAC1 GTPase cycle TAS Reactome
RAC2 GTPase cycle TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Regulation of insulin secretion IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases activate IQGAPs TAS Reactome
RHOA GTPase cycle TAS Reactome
RHOC GTPase cycle TAS Reactome
RHOQ GTPase cycle TAS Reactome
RHOU GTPase cycle TAS Reactome
RHOV GTPase cycle TAS Reactome
Signal Transduction TAS Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by high-kinase activity BRAF mutants TAS Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling downstream of RAS mutants TAS Reactome





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