Gene description for CLIC5
Gene name chloride intracellular channel 5
Gene symbol CLIC5
Other names/aliases DFNB102
DFNB103
MST130
MSTP130
Species Homo sapiens
 Database cross references - CLIC5
ExoCarta ExoCarta_53405
Vesiclepedia VP_53405
Entrez Gene 53405
HGNC 13517
MIM 607293
UniProt Q9NZA1  
 CLIC5 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for CLIC5
Molecular Function
    chloride channel activity GO:0005254 IEA
    protein binding GO:0005515 IPI
    oxidoreductase activity GO:0016491 IEA
Biological Process
    chloride transport GO:0006821 IDA
    female pregnancy GO:0007565 TAS
    visual perception GO:0007601 IEA
    sensory perception of sound GO:0007605 IMP
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    mitochondrial inner membrane GO:0005743 IEA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 IEA
    centrosome GO:0005813 IEA
    cell cortex GO:0005938 IEA
    actin cytoskeleton GO:0015629 IDA
    apical plasma membrane GO:0016324 IEA
    chloride channel complex GO:0034707 IEA
    extracellular exosome GO:0070062 HDA
    stereocilium base GO:0120044 IEA
 Experiment description of studies that identified CLIC5 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CLIC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRN 286262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 FN1 2335
Affinity Capture-MS Homo sapiens
3 EZR 7430
Reconstituted Complex Homo sapiens
4 GPX1 2876
Affinity Capture-MS Homo sapiens
5 HIBCH 26275
Co-fractionation Homo sapiens
6 PLS1 5357
Affinity Capture-MS Homo sapiens
7 MAPK3 5595
Affinity Capture-MS Homo sapiens
8 NME6  
Affinity Capture-MS Homo sapiens
9 CLIC4 25932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MYO5C 55930
Affinity Capture-MS Homo sapiens
11 CLIC2 1193
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MYO5B 4645
Affinity Capture-MS Homo sapiens
13 SRPK1 6732
Biochemical Activity Homo sapiens
14 MAPK1 5594
Affinity Capture-MS Homo sapiens
15 CLIC6 54102
Affinity Capture-MS Homo sapiens
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