Gene description for MYO5B
Gene name myosin VB
Gene symbol MYO5B
Other names/aliases -
Species Homo sapiens
 Database cross references - MYO5B
ExoCarta ExoCarta_4645
Vesiclepedia VP_4645
Entrez Gene 4645
HGNC 7603
MIM 606540
UniProt Q9ULV0  
 MYO5B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 19056867    
 Gene ontology annotations for MYO5B
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    small GTPase binding GO:0031267 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    renal water homeostasis GO:0003091 TAS
    endocytosis GO:0006897 IBA
    actin filament organization GO:0007015 IBA
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IMP
    endosomal transport GO:0016197 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 IBA
    myosin complex GO:0016459 IEA
    cytoplasmic vesicle membrane GO:0030659 TAS
    protein-containing complex GO:0032991 IDA
    apical cortex GO:0045179 IDA
    recycling endosome GO:0055037 IC
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MYO5B in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MYO5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DKK3 27122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LIMA1 51474
Affinity Capture-MS Homo sapiens
5 MYL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 KIAA1549  
Two-hybrid Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 SH3BGRL 6451
Affinity Capture-MS Homo sapiens
9 TRIM54  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 PPP1CB 5500
Affinity Capture-MS Homo sapiens
11 TRIM3 10612
Two-hybrid Homo sapiens
12 TP53 7157
Affinity Capture-MS Homo sapiens
13 EFHC2  
Two-hybrid Homo sapiens
14 OCLM  
Affinity Capture-MS Homo sapiens
15 COIL  
Two-hybrid Homo sapiens
16 DPM2 8818
Affinity Capture-MS Homo sapiens
17 Lima1  
Affinity Capture-MS Mus musculus
18 KIF14 9928
Affinity Capture-MS Homo sapiens
19 ZNHIT1  
Two-hybrid Homo sapiens
20 SSH1  
Proximity Label-MS Homo sapiens
21 DCAF8L2  
Affinity Capture-MS Homo sapiens
22 LRRC39  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RAB11A 8766
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
25 CXCL6  
Affinity Capture-MS Homo sapiens
26 CTNNB1 1499
Affinity Capture-MS Homo sapiens
27 Calml3  
Affinity Capture-MS Mus musculus
28 RAB25 57111
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 Myo1c 17913
Affinity Capture-MS Mus musculus
30 MYO18A 399687
Affinity Capture-MS Homo sapiens
31 SYTL4 94121
Affinity Capture-Western Homo sapiens
32 CALM1 801
Affinity Capture-MS Homo sapiens
33 ERLEC1 27248
Affinity Capture-MS Homo sapiens
34 CAPZA2 830
Affinity Capture-MS Homo sapiens
35 EXOC6 54536
Affinity Capture-Western Homo sapiens
36 NUMA1 4926
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
37 ECT2 1894
Affinity Capture-MS Homo sapiens
38 MYO19  
Affinity Capture-MS Homo sapiens
39 RBX1 9978
Affinity Capture-MS Homo sapiens
40 TCF4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
41 PPP6R2 9701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 Actb 11461
Affinity Capture-MS Mus musculus
43 TMOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 UBE2V2 7336
Affinity Capture-MS Homo sapiens
45 Myh10 77579
Affinity Capture-MS Mus musculus
46 CLIC5 53405
Affinity Capture-MS Homo sapiens
47 EVI5  
Affinity Capture-Western Homo sapiens
48 RAB3B 5865
Affinity Capture-Western Homo sapiens
49 RAB8A 4218
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
50 CAPZB 832
Co-fractionation Homo sapiens
51 SAAL1 113174
Affinity Capture-MS Homo sapiens
52 TNNC1  
Affinity Capture-MS Homo sapiens
53 PTP4A3  
Affinity Capture-MS Homo sapiens
54 POLR1C 9533
Two-hybrid Homo sapiens
55 SYNPO 11346
Affinity Capture-MS Homo sapiens
56 MEI4  
Two-hybrid Homo sapiens
57 BCL2L1 598
Affinity Capture-MS Homo sapiens
58 AMOTL2 51421
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 MYH9 4627
Affinity Capture-MS Homo sapiens
60 STX3 6809
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 KIF23 9493
Affinity Capture-MS Homo sapiens
62 GAGE5  
Affinity Capture-MS Homo sapiens
63 TBC1D30 23329
Affinity Capture-Western Homo sapiens
64 TMOD3 29766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 BNIP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 RAB11FIP2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
67 PARP1 142
Proximity Label-MS Homo sapiens
68 Flot1 14251
Affinity Capture-MS Mus musculus
69 MEOX2  
Two-hybrid Homo sapiens
70 IQGAP1 8826
Affinity Capture-MS Homo sapiens
71 SKA3  
Affinity Capture-MS Homo sapiens
72 TMEM257  
Affinity Capture-MS Homo sapiens
73 CALM3 808
Affinity Capture-MS Homo sapiens
74 AR 367
Affinity Capture-MS Homo sapiens
75 Coro1c 23790
Affinity Capture-MS Mus musculus
76 RAC1 5879
Affinity Capture-MS Homo sapiens
77 RAB11B 9230
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 DBN1 1627
Affinity Capture-MS Homo sapiens
79 CXCR2  
Affinity Capture-Western Homo sapiens
80 Tpm1 22003
Affinity Capture-MS Mus musculus
81 MALL  
Affinity Capture-MS Homo sapiens
82 CCDC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 CASQ2 845
Affinity Capture-MS Homo sapiens
84 PDF  
Affinity Capture-MS Homo sapiens
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