Gene description for MYO5B
Gene name myosin VB
Gene symbol MYO5B
Other names/aliases -
Species Homo sapiens
 Database cross references - MYO5B
ExoCarta ExoCarta_4645
Entrez Gene 4645
HGNC 7603
MIM 606540
UniProt Q9ULV0  
 MYO5B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 19056867    
 Gene ontology annotations for MYO5B
Molecular Function
    Rab GTPase binding GO:0017137 IPI
    microfilament motor activity GO:0000146 TAS
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    actin binding GO:0003779 IEA
Biological Process
    metabolic process GO:0008152 TAS
    vesicle-mediated transport GO:0016192 IMP
    transmembrane transport GO:0055085 TAS
    renal water homeostasis GO:0003091 TAS
    protein transport GO:0015031 IEA
    endosome localization GO:0032439 IMP
    water transport GO:0006833 TAS
Subcellular Localization
    protein complex GO:0043234 IDA
    myosin complex GO:0016459 IEA
    apical cortex GO:0045179 IDA
    extracellular exosome GO:0070062 IDA
    recycling endosome GO:0055037 IC
    cytoplasmic vesicle membrane GO:0030659 TAS
 Experiment description of studies that identified MYO5B in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MYO5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IL8RB  
Affinity Capture-Western Homo sapiens
2 RAB11B 9230
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 RAB11FIP2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 RAB11A 8766
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 RAB25 57111
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MYO5B is involved
PathwayEvidenceSource
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome





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