Experiment description of studies that identified Flot1 in exosomes

1 Experiment ID 1148 MISEV standards ✔ EM EV Biophysical techniques ✔ Flot1|Cd81|Cd9 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 34580290     Organism Mus musculus Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles Authors Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL. Journal name Nat Commun Publication year 2021 Sample Kidney medullary cells Sample name Kidney medullary cells - ciliated Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry small RNA sequencing

2 Experiment ID 1150 MISEV standards ✔ EM EV Biophysical techniques ✔ Flot1|Cd81|Cd9 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 34580290     Organism Mus musculus Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles Authors Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL. Journal name Nat Commun Publication year 2021 Sample Kidney medullary cells Sample name Kidney medullary cells - ciliated Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry small RNA sequencing

3 Experiment ID 39 MISEV standards ✔ IEM EV Biophysical techniques ✔ TSG101|HSC70|FLOT1 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 15210972     Organism Mus musculus Experiment description Cells release prions in association with exosomes. Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G Journal name PNAS Publication year 2004 Sample Mov neuroglial cells Sample name Mov neuroglial cell Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy

4 Experiment ID 870 MISEV standards ✔ EM EV Biophysical techniques ✔ Flot1 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35173168     Organism Mus musculus Experiment description Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression Authors Qi M, Xia Y, Wu Y, Zhang Z, Wang X, Lu L, Dai C, Song Y, Xu K, Ji W, Zhan L. Journal name Nat Commun Publication year 2022 Sample Serum Sample name BALB/c bearing 4T07 primary tumour Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study Western blotting RNA sequencing qRT-PCR

Protein-protein interactions for Flot1

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Flot1 is involved

No pathways found