Gene description for MYO5A
Gene name myosin VA (heavy chain 12, myoxin)
Gene symbol MYO5A
Other names/aliases GS1
MYH12
MYO5
MYR12
Species Homo sapiens
 Database cross references - MYO5A
ExoCarta ExoCarta_4644
Entrez Gene 4644
HGNC 7602
MIM 160777
UniProt Q9Y4I1  
 MYO5A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for MYO5A
Molecular Function
    actin binding GO:0003779 IEA
    Rab GTPase binding GO:0017137 IPI
    calmodulin binding GO:0005516 IEA
    microfilament motor activity GO:0000146 NAS
    ATP binding GO:0005524 IEA
    poly(A) RNA binding GO:0044822 IDA
    calcium ion binding GO:0005509 IEA
Biological Process
    locomotion involved in locomotory behavior GO:0031987 IEA
    long-chain fatty acid biosynthetic process GO:0042759 IEA
    synapse organization GO:0050808 IEA
    cellular response to insulin stimulus GO:0032869 ISS
    secretory granule localization GO:0032252 IEA
    odontogenesis GO:0042476 IEA
    membrane organization GO:0061024 TAS
    vesicle transport along actin filament GO:0030050 IMP
    transport GO:0006810 NAS
    vesicle-mediated transport GO:0016192 ISS
    cellular protein metabolic process GO:0044267 TAS
    exocytosis GO:0006887 IEA
    endoplasmic reticulum localization GO:0051643 IEA
    melanin biosynthetic process GO:0042438 IEA
    visual perception GO:0007601 IEA
    regulation of Golgi organization GO:1903358 IMP
    post-Golgi vesicle-mediated transport GO:0006892 IMP
    protein localization to plasma membrane GO:0072659 ISS
    insulin secretion GO:0030073 IEA
    actin filament-based movement GO:0030048 NAS
    melanocyte differentiation GO:0030318 IEA
    synaptic transmission GO:0007268 IEA
    regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity GO:0031585 IEA
    myelination GO:0042552 IEA
    melanosome transport GO:0032402 IEA
Subcellular Localization
    peroxisome GO:0005777 IDA
    late endosome GO:0005770 IDA
    melanosome GO:0042470 IEA
    extracellular exosome GO:0070062 IDA
    neuronal cell body GO:0043025 IEA
    filopodium tip GO:0032433 IDA
    intermediate filament GO:0005882 IEA
    Golgi apparatus GO:0005794 IEA
    lysosome GO:0005764 IDA
    membrane GO:0016020 IDA
    growth cone GO:0030426 NAS
    myosin complex GO:0016459 IEA
    cytoplasm GO:0005737 IDA
    microtubule plus-end GO:0035371 IEA
    actin filament GO:0005884 IDA
    endoplasmic reticulum GO:0005783 IDA
    ruffle GO:0001726 IDA
    recycling endosome GO:0055037 IDA
    early endosome GO:0005769 IDA
    neuron projection GO:0043005 NAS
    actomyosin GO:0042641 IEA
    insulin-responsive compartment GO:0032593 ISS
    cytosol GO:0005829 IDA
    vesicle GO:0031982 IDA
    photoreceptor outer segment GO:0001750 IEA
 Experiment description of studies that identified MYO5A in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYO5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIM3  
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 SHANK2 22941
Affinity Capture-Western Homo sapiens
3 DLGAP1  
Reconstituted Complex Homo sapiens
4 MLPH  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 BMF  
Reconstituted Complex Homo sapiens
6 DYNLL2 140735
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 RAB27A 5873
Reconstituted Complex Homo sapiens
8 DYNLL1 8655
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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