Gene description for Itga5
Gene name integrin alpha 5 (fibronectin receptor alpha)
Gene symbol Itga5
Other names/aliases Cd49e
Fnra
VLA5
Species Mus musculus
 Database cross references - Itga5
ExoCarta ExoCarta_16402
Entrez Gene 16402
UniProt P11688  
 Itga5 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Itga5
Molecular Function
    epidermal growth factor receptor binding GO:0005154 ISO
    cell adhesion molecule binding GO:0050839 ISO
    metal ion binding GO:0046872 IEA
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cell migration GO:0030335 ISO
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IMP
    cell-substrate adhesion GO:0031589 ISO
    cell-cell adhesion mediated by integrin GO:0033631 IMP
    memory GO:0007613 IMP
    cell adhesion GO:0007155 ISO
    endodermal cell differentiation GO:0035987 ISO
    integrin-mediated signaling pathway GO:0007229 ISO
    heterotypic cell-cell adhesion GO:0034113 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    leukocyte cell-cell adhesion GO:0007159 IMP
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    cell-substrate junction assembly GO:0007044 IDA
    negative regulation of anoikis GO:2000811 ISO
Subcellular Localization
    membrane GO:0016020 IEA
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 ISO
    cytoplasmic vesicle GO:0031410 IDA
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 ISO
    ruffle membrane GO:0032587 ISO
    focal adhesion GO:0005925 ISO
    integral component of membrane GO:0016021 IEA
    synapse GO:0045202 IDA
    endoplasmic reticulum GO:0005783 IDA
    external side of plasma membrane GO:0009897 IDA
    integrin complex GO:0008305 ISO
    cell surface GO:0009986 ISO
 Experiment description of studies that identified Itga5 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Itga5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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