Gene description for Rtn4
Gene name reticulon 4
Gene symbol Rtn4
Other names/aliases 1110020G17Rik
AA407876
AA409940
AA960376
ASY
C130026I10Rik
NOGO
NSP-CL
NgA
Nogo-A
Nogo-B
Nogo-C
mKIAA0886
mKIAA4153
Species Mus musculus
 Database cross references - Rtn4
ExoCarta ExoCarta_68585
Vesiclepedia VP_68585
Entrez Gene 68585
UniProt Q99P72  
 Rtn4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rtn4
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
Biological Process
    regulation of branching morphogenesis of a nerve GO:2000172 ISO
    negative regulation of neuron differentiation GO:0045665 ISO
    positive regulation of glial cell differentiation GO:0045687 ISO
    negative regulation of axonogenesis GO:0050771 ISO
    positive regulation of dopamine secretion GO:0033603 ISO
    cardiac epithelial to mesenchymal transition GO:0060317 IMP
    angiogenesis GO:0001525 IMP
    regulation of nervous system development GO:0051960 IMP
    negative regulation of neuron projection regeneration GO:0070571 ISO
    axonal fasciculation GO:0007413 ISO
    nervous system development GO:0007399 IDA
    regulation of cell migration GO:0030334 ISO
    endoplasmic reticulum tubular network organization GO:0071786 ISO
    cerebral cortex radial glia guided migration GO:0021801 IMP
    negative regulation of cell growth GO:0030308 ISO
    regulation of sensory perception of pain GO:0051930 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    negative regulation of axon extension GO:0030517 ISS
    axonogenesis GO:0007409 IBA
Subcellular Localization
    neuronal postsynaptic density GO:0097481 IDA
    neuronal cell body GO:0043025 ISO
    endoplasmic reticulum GO:0005783 ISO
    protein complex GO:0043234 ISO
    plasma membrane GO:0005886 ISO
    neuron projection GO:0043005 ISO
    nuclear envelope GO:0005635 ISS
    integral component of membrane GO:0016021 IEA
    cell projection GO:0042995 IDA
    intracellular GO:0005622 ISO
    endomembrane system GO:0012505 IBA
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 IEA
    integral component of endoplasmic reticulum membrane GO:0030176 ISS
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 ISO
 Experiment description of studies that identified Rtn4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rtn4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rtn4 is involved
PathwayEvidenceSource
Axonal growth inhibition (RHOA activation) IEA Reactome





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