Gene description for Rtn4
Gene name reticulon 4
Gene symbol Rtn4
Other names/aliases 1110020G17Rik
AA407876
AA409940
AA960376
ASY
C130026I10Rik
NOGO
NSP-CL
NgA
Nogo-A
Nogo-B
Nogo-C
mKIAA0886
mKIAA4153
Species Mus musculus
 Database cross references - Rtn4
ExoCarta ExoCarta_68585
Vesiclepedia VP_68585
Entrez Gene 68585
UniProt Q99P72  
 Rtn4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rtn4
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    protein-containing complex binding GO:0044877 ISO
    cadherin binding GO:0045296 ISO
    metal ion binding GO:0046872 ISO
Biological Process
    blastocyst formation GO:0001825 IMP
    leukocyte migration involved in inflammatory response GO:0002523 IMP
    endoplasmic reticulum organization GO:0007029 ISO
    nervous system development GO:0007399 IDA
    axonal fasciculation GO:0007413 IMP
    axonal fasciculation GO:0007413 ISO
    brain development GO:0007420 IBA
    positive regulation of epithelial cell migration GO:0010634 IEA
    positive regulation of epithelial cell migration GO:0010634 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    cerebral cortex radial glia-guided migration GO:0021801 IMP
    central nervous system vasculogenesis GO:0022009 IMP
    neuron differentiation GO:0030182 IBA
    negative regulation of cell growth GO:0030308 ISO
    regulation of cell migration GO:0030334 ISO
    regulation of cell migration GO:0030334 ISS
    negative regulation of axon extension GO:0030517 IMP
    negative regulation of axon extension GO:0030517 ISO
    negative regulation of axon extension GO:0030517 ISS
    positive regulation of mammary gland epithelial cell proliferation GO:0033601 IEA
    positive regulation of mammary gland epithelial cell proliferation GO:0033601 ISO
    positive regulation of dopamine secretion GO:0033603 ISO
    positive regulation of toll-like receptor 9 signaling pathway GO:0034165 IMP
    positive regulation of Rac protein signal transduction GO:0035022 IMP
    cell migration involved in vasculogenesis GO:0035441 IMP
    negative regulation of neuron differentiation GO:0045665 ISO
    positive regulation of glial cell differentiation GO:0045687 ISO
    positive regulation of angiogenesis GO:0045766 IMP
    negative regulation of axonogenesis GO:0050771 ISO
    modulation of chemical synaptic transmission GO:0050804 EXP
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 ISO
    nuclear pore complex assembly GO:0051292 ISO
    nuclear pore complex assembly GO:0051292 ISS
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    regulation of nervous system development GO:0051960 IMP
    cardiac epithelial to mesenchymal transition GO:0060317 IMP
    positive regulation of macrophage cytokine production GO:0060907 IMP
    protein localization to lysosome GO:0061462 IMP
    negative regulation of neuron projection regeneration GO:0070571 ISO
    cellular response to hypoxia GO:0071456 IDA
    endoplasmic reticulum tubular network organization GO:0071786 ISO
    endoplasmic reticulum tubular network organization GO:0071786 ISS
    endoplasmic reticulum tubular network formation GO:0071787 IBA
    endoplasmic reticulum tubular network formation GO:0071787 ISO
    endoplasmic reticulum tubular network formation GO:0071787 ISS
    intracellular sphingolipid homeostasis GO:0090156 IMP
    cell adhesion involved in sprouting angiogenesis GO:0120078 ISO
    cell adhesion involved in sprouting angiogenesis GO:0120078 ISS
    regulation of postsynapse assembly GO:0150052 EXP
    regulation of postsynapse assembly GO:0150052 IDA
    negative regulation of amyloid-beta formation GO:1902430 ISO
    negative regulation of amyloid-beta formation GO:1902430 ISS
    positive regulation of neutrophil migration GO:1902624 IMP
    positive regulation of macrophage migration GO:1905523 IMP
    positive regulation of protein localization to endoplasmic reticulum GO:1905552 IEA
    positive regulation of protein localization to endoplasmic reticulum GO:1905552 ISO
    positive regulation of ERBB3 signaling pathway GO:1905580 IEA
    positive regulation of ERBB3 signaling pathway GO:1905580 ISO
    positive regulation of artery morphogenesis GO:1905653 IMP
    endoplasmic reticulum tubular network membrane organization GO:1990809 ISO
    endoplasmic reticulum tubular network membrane organization GO:1990809 ISS
    regulation of branching morphogenesis of a nerve GO:2000172 IMP
    regulation of branching morphogenesis of a nerve GO:2000172 ISO
    positive regulation of hepatocyte proliferation GO:2000347 IMP
    negative regulation of vasculogenesis GO:2001213 IMP
Subcellular Localization
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    postsynaptic density GO:0014069 IBA
    postsynaptic density GO:0014069 IDA
    cell junction GO:0030054 ISO
    cell junction GO:0030054 ISS
    protein-containing complex GO:0032991 ISO
    cell projection GO:0042995 IDA
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 ISO
    synapse GO:0045202 IDA
    anchoring junction GO:0070161 IEA
    endoplasmic reticulum tubular network GO:0071782 ISO
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    postsynapse GO:0098794 ISO
    endoplasmic reticulum tubular network membrane GO:0098826 ISO
    endoplasmic reticulum tubular network membrane GO:0098826 ISS
    postsynaptic density membrane GO:0098839 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rtn4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rtn4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itgav 16410
Affinity Capture-Western Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Itga5 16402
Affinity Capture-Western Mus musculus
5 Traf3  
Proximity Label-MS Mus musculus
6 Rtn4r  
Affinity Capture-Western Mus musculus
7 Pou5f1  
Affinity Capture-MS Mus musculus
8 Unk  
Affinity Capture-RNA Mus musculus
9 Rnf41  
Two-hybrid Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here