Gene description for Itgav
Gene name integrin alpha V
Gene symbol Itgav
Other names/aliases 1110004F14Rik
2610028E01Rik
CD51
D430040G12Rik
Species Mus musculus
 Database cross references - Itgav
ExoCarta ExoCarta_16410
Vesiclepedia VP_16410
Entrez Gene 16410
UniProt P43406  
 Itgav identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itgav
Molecular Function
    opsonin binding GO:0001846 IDA
    fibronectin binding GO:0001968 IEA
    fibronectin binding GO:0001968 ISO
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    protein kinase C binding GO:0005080 IDA
    signaling receptor binding GO:0005102 ISO
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IPI
    integrin binding GO:0005178 ISO
    voltage-gated calcium channel activity GO:0005245 IEA
    voltage-gated calcium channel activity GO:0005245 ISO
    protein binding GO:0005515 IPI
    fibroblast growth factor binding GO:0017134 IEA
    fibroblast growth factor binding GO:0017134 ISO
    C-X3-C chemokine binding GO:0019960 ISO
    C-X3-C chemokine binding GO:0019960 ISS
    insulin-like growth factor I binding GO:0031994 ISO
    insulin-like growth factor I binding GO:0031994 ISS
    neuregulin binding GO:0038132 ISO
    neuregulin binding GO:0038132 ISS
    metal ion binding GO:0046872 IEA
    transforming growth factor beta binding GO:0050431 ISS
    extracellular matrix binding GO:0050840 IEA
    extracellular matrix binding GO:0050840 ISO
    extracellular matrix protein binding GO:1990430 IEA
    extracellular matrix protein binding GO:1990430 ISO
Biological Process
    angiogenesis GO:0001525 IBA
    angiogenesis GO:0001525 IEA
    blood vessel development GO:0001568 IMP
    vasculogenesis GO:0001570 IEA
    inflammatory response GO:0006954 TAS
    cell adhesion GO:0007155 ISO
    cell adhesion GO:0007155 ISS
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 ISO
    cell-matrix adhesion GO:0007160 NAS
    cell-matrix adhesion GO:0007160 TAS
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 ISO
    integrin-mediated signaling pathway GO:0007229 NAS
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    fertilization GO:0009566 NAS
    negative regulation of macrophage derived foam cell differentiation GO:0010745 ISO
    negative regulation of macrophage derived foam cell differentiation GO:0010745 ISS
    negative regulation of lipid storage GO:0010888 ISO
    negative regulation of lipid storage GO:0010888 ISS
    cell migration GO:0016477 ISO
    positive regulation of cell migration GO:0030335 IMP
    cell-substrate adhesion GO:0031589 ISO
    negative regulation of lipid transport GO:0032369 ISO
    negative regulation of lipid transport GO:0032369 ISS
    detection of molecule of bacterial origin GO:0032490 TAS
    cell adhesion mediated by integrin GO:0033627 IBA
    cell adhesion mediated by integrin GO:0033627 IDA
    cell adhesion mediated by integrin GO:0033627 ISO
    cell adhesion mediated by integrin GO:0033627 ISS
    positive regulation of osteoblast proliferation GO:0033690 IMP
    heterotypic cell-cell adhesion GO:0034113 IEA
    heterotypic cell-cell adhesion GO:0034113 ISO
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    gonad morphogenesis GO:0035262 NAS
    wound healing, spreading of epidermal cells GO:0035313 NAS
    endodermal cell differentiation GO:0035987 IEA
    endodermal cell differentiation GO:0035987 ISO
    apolipoprotein A-I-mediated signaling pathway GO:0038027 IEA
    apolipoprotein A-I-mediated signaling pathway GO:0038027 ISO
    T cell activation GO:0042110 TAS
    apoptotic cell clearance GO:0043277 IDA
    apoptotic cell clearance GO:0043277 IGI
    apoptotic cell clearance GO:0043277 IMP
    apoptotic cell clearance GO:0043277 ISO
    endothelial cell migration GO:0043542 TAS
    regulation of bone resorption GO:0045124 TAS
    positive regulation of cell adhesion GO:0045785 ISO
    positive regulation of cell adhesion GO:0045785 ISS
    symbiont entry into host cell GO:0046718 IEA
    symbiont entry into host cell GO:0046718 ISO
    focal adhesion assembly GO:0048041 NAS
    negative regulation of lipoprotein metabolic process GO:0050748 ISO
    negative regulation of lipoprotein metabolic process GO:0050748 ISS
    regulation of phagocytosis GO:0050764 ISO
    regulation of phagocytosis GO:0050764 ISS
    regulation of phagocytosis GO:0050764 NAS
    negative chemotaxis GO:0050919 IEA
    negative chemotaxis GO:0050919 ISO
    positive regulation of small GTPase mediated signal transduction GO:0051057 IEA
    positive regulation of small GTPase mediated signal transduction GO:0051057 ISO
    release of sequestered calcium ion into cytosol GO:0051209 TAS
    trophoblast giant cell differentiation GO:0060707 TAS
    ERK1 and ERK2 cascade GO:0070371 IDA
    calcium ion transmembrane transport GO:0070588 ISO
    transforming growth factor beta production GO:0071604 IDA
    response to nitric oxide GO:0071731 TAS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IDA
    cell-cell adhesion GO:0098609 IBA
    positive regulation of intracellular signal transduction GO:1902533 ISO
    negative regulation of low-density lipoprotein receptor activity GO:1905598 ISO
    negative regulation of low-density lipoprotein receptor activity GO:1905598 ISS
    negative regulation of entry of bacterium into host cell GO:2000536 ISO
    negative regulation of entry of bacterium into host cell GO:2000536 ISS
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IEA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 ISO
    focal adhesion GO:0005925 ISS
    integrin complex GO:0008305 IBA
    integrin complex GO:0008305 ISO
    integrin complex GO:0008305 ISS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    cell surface GO:0009986 ISS
    membrane GO:0016020 IDA
    lamellipodium membrane GO:0031258 IEA
    lamellipodium membrane GO:0031258 ISO
    filopodium membrane GO:0031527 IEA
    filopodium membrane GO:0031527 ISO
    microvillus membrane GO:0031528 IEA
    microvillus membrane GO:0031528 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    integrin alphav-beta1 complex GO:0034682 IEA
    integrin alphav-beta1 complex GO:0034682 ISO
    integrin alphav-beta3 complex GO:0034683 ISO
    integrin alphav-beta3 complex GO:0034683 TAS
    integrin alphav-beta5 complex GO:0034684 IDA
    integrin alphav-beta5 complex GO:0034684 ISO
    integrin alphav-beta6 complex GO:0034685 IDA
    integrin alphav-beta6 complex GO:0034685 ISO
    integrin alphav-beta8 complex GO:0034686 ISO
    integrin alphav-beta8 complex GO:0034686 ISS
    alphav-beta3 integrin-IGF-1-IGF1R complex GO:0035867 IEA
    alphav-beta3 integrin-IGF-1-IGF1R complex GO:0035867 ISO
    alphav-beta3 integrin-HMGB1 complex GO:0035868 IEA
    alphav-beta3 integrin-HMGB1 complex GO:0035868 ISO
 Experiment description of studies that identified Itgav in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
11
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itgav
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rtn4 68585
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Itgav is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Antigen processing-Cross presentation IEA Reactome
Axon guidance IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
Cross-presentation of particulate exogenous antigens (phagosomes) IEA Reactome
Developmental Biology IEA Reactome
ECM proteoglycans IEA Reactome
Elastic fibre formation IEA Reactome
Extracellular matrix organization IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Integrin cell surface interactions IEA Reactome
L1CAM interactions IEA Reactome
Molecules associated with elastic fibres IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Non-integrin membrane-ECM interactions IEA Reactome
PECAM1 interactions IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Signaling by VEGF IEA Reactome
Syndecan interactions IEA Reactome
TGF-beta receptor signaling activates SMADs IEA Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





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