Gene description for SPECC1L
Gene name sperm antigen with calponin homology and coiled-coil domains 1-like
Gene symbol SPECC1L
Other names/aliases CYTSA
GBBB2
OBLFC1
Species Homo sapiens
 Database cross references - SPECC1L
ExoCarta ExoCarta_23384
Vesiclepedia VP_23384
Entrez Gene 23384
HGNC 29022
MIM 614140
UniProt Q69YQ0  
 SPECC1L identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SPECC1L
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 IDA
    actin cytoskeleton organization GO:0030036 IBA
    cell division GO:0051301 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    microtubule organizing center GO:0005815 IBA
    spindle GO:0005819 IEA
    gap junction GO:0005921 IEA
    actin cytoskeleton GO:0015629 IDA
    filamentous actin GO:0031941 IBA
 Experiment description of studies that identified SPECC1L in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SPECC1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLFN11 91607
Proximity Label-MS Homo sapiens
2 CHMP4C 92421
Affinity Capture-MS Homo sapiens
3 LUZP4  
Affinity Capture-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 DUSP22  
Affinity Capture-MS Homo sapiens
7 ARPC2 10109
Affinity Capture-MS Homo sapiens
8 BTF3 689
Affinity Capture-MS Homo sapiens
9 Calml3  
Affinity Capture-MS Mus musculus
10 APC  
Two-hybrid Homo sapiens
11 PTPRZ1  
Affinity Capture-MS Homo sapiens
12 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
13 RPL15 6138
Affinity Capture-MS Homo sapiens
14 UQCRB 7381
Affinity Capture-MS Homo sapiens
15 UBC 7316
Reconstituted Complex Homo sapiens
16 CSTA 1475
Affinity Capture-MS Homo sapiens
17 MYO5C 55930
Affinity Capture-MS Homo sapiens
18 Actb 11461
Affinity Capture-MS Mus musculus
19 LRRC27  
Affinity Capture-MS Homo sapiens
20 CAPZB 832
Affinity Capture-MS Homo sapiens
21 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
22 RDX 5962
Proximity Label-MS Homo sapiens
23 IRAK1BP1 134728
Affinity Capture-MS Homo sapiens
24 FAM57A  
Affinity Capture-MS Homo sapiens
25 CALD1 800
Affinity Capture-MS Homo sapiens
26 FBXW7  
Affinity Capture-MS Homo sapiens
27 PRC1 9055
Affinity Capture-MS Homo sapiens
28 IQGAP1 8826
Affinity Capture-MS Homo sapiens
29 CALM3 808
Affinity Capture-MS Homo sapiens
30 Ankrd26  
Affinity Capture-MS Mus musculus
31 YWHAG 7532
Affinity Capture-MS Homo sapiens
32 DNAJB1 3337
Affinity Capture-MS Homo sapiens
33 SOGA3  
Affinity Capture-MS Homo sapiens
34 ZNF219  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ATG16L1 55054
Affinity Capture-MS Homo sapiens
36 PKN3 29941
Affinity Capture-MS Homo sapiens
37 C10orf53  
Affinity Capture-MS Homo sapiens
38 RCN1 5954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 Lima1  
Affinity Capture-MS Mus musculus
40 KIF14 9928
Affinity Capture-MS Homo sapiens
41 CACNB3  
Affinity Capture-MS Homo sapiens
42 FSCN2  
Affinity Capture-MS Homo sapiens
43 KRT34  
Affinity Capture-MS Homo sapiens
44 MYO18A 399687
Affinity Capture-MS Homo sapiens
45 POLD1 5424
Affinity Capture-MS Homo sapiens
46 CCNJ  
Affinity Capture-MS Homo sapiens
47 VAV1 7409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GLIS2  
Two-hybrid Homo sapiens
49 MAPRE1 22919
Affinity Capture-MS Homo sapiens
50 RNF38  
Affinity Capture-MS Homo sapiens
51 UBE2I 7329
Two-hybrid Homo sapiens
52 CHMP4B 128866
Affinity Capture-MS Homo sapiens
53 MYH9 4627
Affinity Capture-MS Homo sapiens
54 ACTN4 81
Affinity Capture-MS Homo sapiens
55 DCTN1 1639
Proximity Label-MS Homo sapiens
56 CCDC8  
Affinity Capture-MS Homo sapiens
57 YWHAQ 10971
Affinity Capture-MS Homo sapiens
58 FBXO25  
Affinity Capture-MS Homo sapiens
59 COPS3 8533
Affinity Capture-MS Homo sapiens
60 EPB41L5 57669
Affinity Capture-MS Homo sapiens
61 DBN1 1627
Affinity Capture-MS Homo sapiens
62 FGFR1OP  
Affinity Capture-MS Homo sapiens
63 G2E3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 INTS4P2  
Affinity Capture-MS Homo sapiens
65 LRFN4  
Affinity Capture-MS Homo sapiens
66 YWHAH 7533
Affinity Capture-MS Homo sapiens
67 SDHD  
Affinity Capture-MS Homo sapiens
68 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 FAM3D  
Affinity Capture-MS Homo sapiens
70 Myh9 17886
Affinity Capture-MS Mus musculus
71 KIF20A 10112
Affinity Capture-MS Homo sapiens
72 ECT2 1894
Affinity Capture-MS Homo sapiens
73 CTNNB1 1499
Affinity Capture-MS Homo sapiens
74 NFKBIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 CTTN 2017
Affinity Capture-MS Homo sapiens
76 C11orf49  
Affinity Capture-MS Homo sapiens
77 SLC30A6 55676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 SURF6  
Affinity Capture-MS Homo sapiens
79 Itga5 16402
Affinity Capture-MS Mus musculus
80 ERLEC1 27248
Affinity Capture-MS Homo sapiens
81 SRSF5 6430
Affinity Capture-MS Homo sapiens
82 MYO19  
Affinity Capture-MS Homo sapiens
83 OBFC1 79991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 ATMIN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 ZC3H10  
Affinity Capture-MS Homo sapiens
87 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 MAPRE3  
Proximity Label-MS Homo sapiens
89 CHRNA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 MYC  
Affinity Capture-MS Homo sapiens
91 Uso1 56041
Affinity Capture-MS Mus musculus
92 RPS14 6208
Affinity Capture-MS Homo sapiens
93 KIAA0368 23392
Two-hybrid Homo sapiens
94 RNF26  
Affinity Capture-MS Homo sapiens
95 Ppp1cb 19046
Affinity Capture-MS Mus musculus
96 NADK2 133686
Affinity Capture-MS Homo sapiens
97 C10orf2 56652
Affinity Capture-MS Homo sapiens
98 ZRANB1 54764
Affinity Capture-MS Homo sapiens
99 FEZ1  
Affinity Capture-MS Homo sapiens
100 PDLIM7 9260
Affinity Capture-MS Homo sapiens
101 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 CCND2  
Affinity Capture-MS Homo sapiens
103 PDE7A  
Affinity Capture-MS Homo sapiens
104 Myo1c 17913
Affinity Capture-MS Mus musculus
105 CAPZA2 830
Affinity Capture-MS Homo sapiens
106 FSD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 CDK2 1017
Affinity Capture-MS Homo sapiens
108 FMNL3 91010
Affinity Capture-MS Homo sapiens
109 PPFIBP2 8495
Affinity Capture-MS Homo sapiens
110 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 Myh10 77579
Affinity Capture-MS Mus musculus
112 SPC25 57405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 Samm50  
Affinity Capture-MS Mus musculus
114 CENPK  
Affinity Capture-MS Homo sapiens
115 TMEM257  
Affinity Capture-MS Homo sapiens
116 SYNPO 11346
Affinity Capture-MS Homo sapiens
117 PINK1  
Affinity Capture-MS Homo sapiens
118 CIT 11113
Affinity Capture-MS Homo sapiens
119 RPL8 6132
Affinity Capture-MS Homo sapiens
120 KCTD17 79734
Affinity Capture-MS Homo sapiens
121 QRSL1  
Affinity Capture-MS Homo sapiens
122 PTRF 284119
Affinity Capture-MS Homo sapiens
123 AIFM2 84883
Affinity Capture-MS Homo sapiens
124 FAM133A 286499
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPECC1L is involved
No pathways found





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