Gene description for AIFM2
Gene name apoptosis-inducing factor, mitochondrion-associated, 2
Gene symbol AIFM2
Other names/aliases AMID
PRG3
Species Homo sapiens
 Database cross references - AIFM2
ExoCarta ExoCarta_84883
Entrez Gene 84883
HGNC 21411
MIM 605159
UniProt Q9BRQ8  
 AIFM2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for AIFM2
Molecular Function
    DNA binding GO:0003677 IDA
    electron-transferring-flavoprotein dehydrogenase activity GO:0004174 IDA
    NADH dehydrogenase (ubiquinone) activity GO:0008137 IBA
    flavin adenine dinucleotide binding GO:0050660 IDA
Biological Process
    apoptotic mitochondrial changes GO:0008637 IDA
    oxidation-reduction process GO:0055114 IEA
    positive regulation of apoptotic process GO:0043065 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
    mitochondrial outer membrane GO:0005741 IDA
    extracellular space GO:0005615 IDA
    integral component of membrane GO:0016021 IEA
    lipid particle GO:0005811 IDA
    mitochondrion GO:0005739 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified AIFM2 in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AIFM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AIFM2 is involved
No pathways found





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