Gene description for NMT2
Gene name N-myristoyltransferase 2
Gene symbol NMT2
Other names/aliases -
Species Homo sapiens
 Database cross references - NMT2
ExoCarta ExoCarta_9397
Vesiclepedia VP_9397
Entrez Gene 9397
HGNC 7858
MIM 603801
UniProt O60551  
 NMT2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NMT2
Molecular Function
    glycylpeptide N-tetradecanoyltransferase activity GO:0004379 IBA
    glycylpeptide N-tetradecanoyltransferase activity GO:0004379 IDA
    glycylpeptide N-tetradecanoyltransferase activity GO:0004379 TAS
    protein binding GO:0005515 IPI
    peptidyl-lysine N6-myristoyltransferase activity GO:0018030 IDA
Biological Process
    N-terminal peptidyl-glycine N-myristoylation GO:0018008 IDA
    regulation of opsin-mediated signaling pathway GO:0022400 TAS
    protein localization to membrane GO:0072657 IBA
    intracellular transport of virus GO:0075733 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    host cell GO:0043657 IEA
 Experiment description of studies that identified NMT2 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NMT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MIIP 60672
Affinity Capture-MS Homo sapiens
2 PSD2  
Affinity Capture-MS Homo sapiens
3 ARMC3 219681
Affinity Capture-MS Homo sapiens
4 FAM84A  
Affinity Capture-MS Homo sapiens
5 GPRC5C 55890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FAM45A 404636
Affinity Capture-MS Homo sapiens
7 CSNK2B 1460
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 SMN1 6606
Two-hybrid Homo sapiens
9 TMED9 54732
Two-hybrid Homo sapiens
10 TK1 7083
Two-hybrid Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 TP53 7157
Affinity Capture-Western Homo sapiens
13 AIFM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PEX19 5824
Affinity Capture-MS Homo sapiens
15 ODF3L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CADM4 199731
Affinity Capture-MS Homo sapiens
17 BTF3 689
Two-hybrid Homo sapiens
18 MGRN1 23295
Affinity Capture-MS Homo sapiens
19 TUSC2  
Affinity Capture-MS Homo sapiens
20 ANXA13 312
Affinity Capture-MS Homo sapiens
21 ANXA7 310
Two-hybrid Homo sapiens
22 RP2 6102
Affinity Capture-MS Homo sapiens
23 S100A2 6273
Affinity Capture-MS Homo sapiens
24 GFOD1  
Affinity Capture-MS Homo sapiens
25 ASB6 140459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PRKCZ 5590
Two-hybrid Homo sapiens
27 ZNRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
29 ARL11  
Affinity Capture-MS Homo sapiens
30 ARF6 382
Affinity Capture-MS Homo sapiens
31 TLDC1 57707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 APP 351
Reconstituted Complex Homo sapiens
33 FAM84B 157638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PRPS2 5634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PPP1CC 5501
Two-hybrid Homo sapiens
36 FAM109B  
Affinity Capture-MS Homo sapiens
37 ANGPTL4 51129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CYS1 192668
Affinity Capture-MS Homo sapiens
39 ERICH5  
Affinity Capture-MS Homo sapiens
40 BCL2  
Affinity Capture-Western Homo sapiens
41 NCS1 23413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TGFB1 7040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 DEFB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ACBD6 84320
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
45 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
46 ANXA4 307
Two-hybrid Homo sapiens
47 C9orf170  
Affinity Capture-MS Homo sapiens
48 FBXO17 115290
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CHURC1  
Affinity Capture-MS Homo sapiens
50 SLC31A1 1317
Affinity Capture-MS Homo sapiens
51 RFTN2 130132
Affinity Capture-MS Homo sapiens
52 PRPS1 5631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 HPCA 3208
Affinity Capture-MS Homo sapiens
54 AIFM2 84883
Affinity Capture-MS Homo sapiens
55 MGST3 4259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 EP300 2033
Affinity Capture-MS Homo sapiens
57 CT45A5  
Affinity Capture-MS Homo sapiens
58 MARCKSL1 65108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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