Gene description for ANGPTL4
Gene name angiopoietin-like 4
Gene symbol ANGPTL4
Other names/aliases ANGPTL2
ARP4
FIAF
HARP
HFARP
NL2
PGAR
TGQTL
UNQ171
pp1158
Species Homo sapiens
 Database cross references - ANGPTL4
ExoCarta ExoCarta_51129
Vesiclepedia VP_51129
Entrez Gene 51129
HGNC 16039
MIM 605910
UniProt Q9BY76  
 ANGPTL4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast milk 17641064    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ANGPTL4
Molecular Function
    enzyme inhibitor activity GO:0004857 IBA
    enzyme inhibitor activity GO:0004857 ISS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    angiogenesis GO:0001525 IEA
    response to hypoxia GO:0001666 NAS
    lipid metabolic process GO:0006629 IEA
    blood coagulation GO:0007596 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    protein unfolding GO:0043335 IDA
    positive regulation of angiogenesis GO:0045766 TAS
    negative regulation of lipoprotein lipase activity GO:0051005 IDA
    triglyceride homeostasis GO:0070328 IBA
    triglyceride homeostasis GO:0070328 ISS
    endothelial cell apoptotic process GO:0072577 IEA
    negative regulation of endothelial cell apoptotic process GO:2000352 IEA
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    collagen-containing extracellular matrix GO:0062023 IBA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified ANGPTL4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANGPTL4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PM20D2 135293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 AASDHPPT 60496
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NMT2 9397
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ACTB 60
Co-purification Homo sapiens
5 LAMB1 3912
Affinity Capture-MS Homo sapiens
6 SMARCA4 6597
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
7 NMT1 4836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 C12orf43  
Affinity Capture-MS Homo sapiens
9 MYC  
Co-purification Homo sapiens
10 ITIH2 3698
Affinity Capture-MS Homo sapiens
11 MKLN1  
Affinity Capture-MS Homo sapiens
12 DNAJB4 11080
Affinity Capture-MS Homo sapiens
13 WDR47  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 SNAP29 9342
Affinity Capture-MS Homo sapiens
15 ARMC8 25852
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CWC15  
Affinity Capture-MS Homo sapiens
17 KAT5  
Co-purification Homo sapiens
View the network image/svg+xml



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