Experiment description of studies that identified PM20D2 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

4 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for PM20D2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ARNTL   Affinity Capture-MS Homo sapiens 2 PRIM2 5558 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 UBE2H 7328 Affinity Capture-MS Homo sapiens 4 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 5 RGS12 6002 Two-hybrid Homo sapiens 6 TK1 7083 Affinity Capture-MS Homo sapiens 7 LNP1   Affinity Capture-MS Homo sapiens 8 DALRD3   Two-hybrid Homo sapiens 9 TAPBPL 55080 Two-hybrid Homo sapiens 10 SPATA24   Affinity Capture-MS Homo sapiens 11 DNAJA2 10294 Proximity Label-MS Homo sapiens 12 CA11   Affinity Capture-MS Homo sapiens 13 FAM154A   Two-hybrid Homo sapiens 14 REL 5966 Two-hybrid Homo sapiens 15 ZSCAN1   Affinity Capture-MS Homo sapiens 16 RP2 6102 Two-hybrid Homo sapiens 17 ANG 283 Affinity Capture-MS Homo sapiens 18 NUDT6   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 MRPL37 51253 Affinity Capture-MS Homo sapiens 20 NIPAL1   Affinity Capture-MS Homo sapiens 21 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 22 MYOZ3   Two-hybrid Homo sapiens 23 PARK2   Affinity Capture-MS Homo sapiens 24 FUS 2521 Affinity Capture-MS Homo sapiens 25 DDIT4L   Two-hybrid Homo sapiens 26 PM20D2 135293 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 27 HAPLN2   Two-hybrid Homo sapiens 28 PSMA1 5682 Two-hybrid Homo sapiens 29 TDO2   Two-hybrid Homo sapiens 30 CNNM3 26505 Two-hybrid Homo sapiens 31 KEL   Two-hybrid Homo sapiens 32 ANGPTL4 51129 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 POU6F2   Two-hybrid Homo sapiens 34 PTPN21 11099 Affinity Capture-MS Homo sapiens 35 KRTAP26-1   Two-hybrid Homo sapiens 36 CCDC69 26112 Affinity Capture-MS Homo sapiens 37 HAO2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 OGT 8473 Reconstituted Complex Homo sapiens 39 DEFB1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 KRT75 9119 Two-hybrid Homo sapiens 41 SPATA2   Affinity Capture-MS Homo sapiens 42 GPSM3   Affinity Capture-MS Homo sapiens 43 COL4A3BP 10087 Two-hybrid Homo sapiens 44 KIAA1671   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 NAT6   Two-hybrid Homo sapiens 46 KBTBD4   Affinity Capture-MS Homo sapiens 47 ADCYAP1   Affinity Capture-MS Homo sapiens 48 C1orf158   Affinity Capture-MS Homo sapiens 49 SIVA1   Two-hybrid Homo sapiens 50 TEKT2   Affinity Capture-MS Homo sapiens 51 RUSC1   Two-hybrid Homo sapiens 52 TULP3 7289 Affinity Capture-MS Homo sapiens 53 ZKSCAN4   Affinity Capture-MS Homo sapiens View the network

Pathways in which PM20D2 is involved

No pathways found