Gene description for CNNM3
Gene name cyclin and CBS domain divalent metal cation transport mediator 3
Gene symbol CNNM3
Other names/aliases ACDP3
Species Homo sapiens
 Database cross references - CNNM3
ExoCarta ExoCarta_26505
Vesiclepedia VP_26505
Entrez Gene 26505
HGNC 104
MIM 607804
UniProt Q8NE01  
 CNNM3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CNNM3
Molecular Function
    protein binding GO:0005515 IPI
    transmembrane transporter activity GO:0022857 IBA
Biological Process
    monoatomic ion transport GO:0006811 IEA
    magnesium ion homeostasis GO:0010960 IBA
    transmembrane transport GO:0055085 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified CNNM3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CNNM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 P4HB 5034
Two-hybrid Homo sapiens
3 KRTAP12-3  
Two-hybrid Homo sapiens
4 KHDRBS3  
Two-hybrid Homo sapiens
5 RBMY1F  
Two-hybrid Homo sapiens
6 RBPMS 11030
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 LCN6  
Affinity Capture-MS Homo sapiens
9 METTL7A 25840
Proximity Label-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 NOTCH2NL 388677
Two-hybrid Homo sapiens
12 SPERT  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 ADRB2  
Affinity Capture-MS Homo sapiens
14 MDFI  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 PTP4A1 7803
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 KRTAP5-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 KRTAP1-1  
Two-hybrid Homo sapiens
18 STX4 6810
Proximity Label-MS Homo sapiens
19 SLC22A9  
Affinity Capture-MS Homo sapiens
20 UBE2G2  
Two-hybrid Homo sapiens
21 AGR3 155465
Two-hybrid Homo sapiens
22 KRTAP4-12  
Two-hybrid Homo sapiens
23 SSC4D  
Two-hybrid Homo sapiens
24 ASIC1  
Affinity Capture-MS Homo sapiens
25 KRTAP2-4  
Two-hybrid Homo sapiens
26 KRT40  
Two-hybrid Homo sapiens
27 STX7 8417
Proximity Label-MS Homo sapiens
28 CALCOCO2  
Two-hybrid Homo sapiens
29 SSR1 6745
Proximity Label-MS Homo sapiens
30 MTUS2 23281
Two-hybrid Homo sapiens
31 USHBP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 KPRP 448834
Two-hybrid Homo sapiens
33 ILK 3611
Affinity Capture-MS Homo sapiens
34 RAB35 11021
Proximity Label-MS Homo sapiens
35 CCNDBP1 23582
Two-hybrid Homo sapiens
36 BTNL2  
Affinity Capture-MS Homo sapiens
37 MARCKS 4082
Proximity Label-MS Homo sapiens
38 LAMTOR1 55004
Proximity Label-MS Homo sapiens
39 RBMX 27316
Two-hybrid Homo sapiens
40 GOLGA6L9  
Two-hybrid Homo sapiens
41 EFNA2  
Affinity Capture-MS Homo sapiens
42 FHL5 9457
Two-hybrid Homo sapiens
43 FHL3 2275
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 ZNF330  
Two-hybrid Homo sapiens
45 SRSF3 6428
Two-hybrid Homo sapiens
46 LYN 4067
Proximity Label-MS Homo sapiens
47 MCAM 4162
Proximity Label-MS Homo sapiens
48 DNAJC5 80331
Proximity Label-MS Homo sapiens
49 PTP4A3  
Affinity Capture-MS Homo sapiens
50 PNMA3  
Two-hybrid Homo sapiens
51 DIRAS3  
Proximity Label-MS Homo sapiens
52 CYSRT1 375791
Two-hybrid Homo sapiens
53 KRTAP6-3  
Two-hybrid Homo sapiens
54 PTP4A2 8073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 HLA-C 3107
Affinity Capture-MS Homo sapiens
56 PNMA2  
Two-hybrid Homo sapiens
57 RAB2A 5862
Proximity Label-MS Homo sapiens
58 AGR2 10551
Two-hybrid Homo sapiens
59 CD160  
Affinity Capture-MS Homo sapiens
60 ARL15 54622
Affinity Capture-MS Homo sapiens
61 CTDSPL 10217
Proximity Label-MS Homo sapiens
62 LY86  
Affinity Capture-MS Homo sapiens
63 KRTAP9-8  
Two-hybrid Homo sapiens
64 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
65 KRTAP1-3  
Two-hybrid Homo sapiens
66 CANX 821
Affinity Capture-MS Homo sapiens
67 KRT33B 3884
Two-hybrid Homo sapiens
68 TNFSF8  
Affinity Capture-MS Homo sapiens
69 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 LAMP1 3916
Proximity Label-MS Homo sapiens
71 KRTAP9-3  
Two-hybrid Homo sapiens
72 TSPAN18 90139
Affinity Capture-MS Homo sapiens
73 ARF6 382
Proximity Label-MS Homo sapiens
74 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 HOXA1 3198
Two-hybrid Homo sapiens
76 VWC2  
Two-hybrid Homo sapiens
77 PM20D2 135293
Two-hybrid Homo sapiens
78 TMEM173  
Affinity Capture-MS Homo sapiens
79 COG6 57511
Two-hybrid Homo sapiens
80 TSPAN8 7103
Affinity Capture-MS Homo sapiens
81 GML  
Affinity Capture-MS Homo sapiens
82 TRIM27  
Two-hybrid Homo sapiens
83 TNS2 23371
Two-hybrid Homo sapiens
84 KRTAP9-2  
Two-hybrid Homo sapiens
85 CST9L  
Affinity Capture-MS Homo sapiens
86 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
87 RHOB 388
Proximity Label-MS Homo sapiens
88 KRTAP3-2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
89 FLOT1 10211
Proximity Label-MS Homo sapiens
90 KRTAP10-5  
Two-hybrid Homo sapiens
91 CCDC8  
Affinity Capture-MS Homo sapiens
92 RBMY1J  
Two-hybrid Homo sapiens
93 RELA 5970
Two-hybrid Homo sapiens
94 UBQLN2 29978
Two-hybrid Homo sapiens
95 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
96 FASN 2194
Negative Genetic Homo sapiens
97 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
98 CLEC2D  
Affinity Capture-MS Homo sapiens
99 NBPF19  
Two-hybrid Homo sapiens
100 RYK 6259
Affinity Capture-MS Homo sapiens
101 TSPAN17 26262
Affinity Capture-MS Homo sapiens
102 LZTS2 84445
Two-hybrid Homo sapiens
103 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
104 WWOX 51741
Two-hybrid Homo sapiens
105 PNMA1 9240
Two-hybrid Homo sapiens
106 C11orf52 91894
Proximity Label-MS Homo sapiens
107 APOM 55937
Affinity Capture-MS Homo sapiens
108 HNRNPK 3190
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
109 KRTAP17-1  
Two-hybrid Homo sapiens
110 SFTPC  
Affinity Capture-MS Homo sapiens
111 KHDRBS2  
Two-hybrid Homo sapiens
112 EFEMP2 30008
Two-hybrid Homo sapiens
113 BRICD5  
Affinity Capture-MS Homo sapiens
114 KRTAP12-2  
Two-hybrid Homo sapiens
115 RAB11A 8766
Proximity Label-MS Homo sapiens
116 KRTAP3-1  
Two-hybrid Homo sapiens
117 EPHA2 1969
Proximity Label-MS Homo sapiens
118 CST11  
Affinity Capture-MS Homo sapiens
119 ASIC4  
Affinity Capture-MS Homo sapiens
120 KRAS 3845
Proximity Label-MS Homo sapiens
121 HSF2BP  
Two-hybrid Homo sapiens
122 PTCH1  
Affinity Capture-MS Homo sapiens
123 CAV1 857
Proximity Label-MS Homo sapiens
124 EXOSC8  
Two-hybrid Homo sapiens
125 PCDHB3  
Affinity Capture-MS Homo sapiens
126 KRTAP10-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CNNM3 is involved
No pathways found





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