Gene description for RYK
Gene name receptor-like tyrosine kinase
Gene symbol RYK
Other names/aliases D3S3195
JTK5
JTK5A
RYK1
Species Homo sapiens
 Database cross references - RYK
ExoCarta ExoCarta_6259
Vesiclepedia VP_6259
Entrez Gene 6259
HGNC 10481
MIM 600524
UniProt P34925  
 RYK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RYK
Molecular Function
    protein tyrosine kinase activity GO:0004713 IDA
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IBA
    transmembrane signaling receptor activity GO:0004888 IDA
    frizzled binding GO:0005109 ISS
    frizzled binding GO:0005109 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    coreceptor activity GO:0015026 TAS
    Wnt-protein binding GO:0017147 ISS
    Wnt-protein binding GO:0017147 NAS
    Wnt receptor activity GO:0042813 ISS
Biological Process
    protein phosphorylation GO:0006468 IDA
    signal transduction GO:0007165 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    multicellular organism development GO:0007275 IBA
    axonogenesis GO:0007409 IBA
    axonogenesis GO:0007409 ISS
    axonogenesis GO:0007409 TAS
    axon guidance GO:0007411 ISS
    axon guidance GO:0007411 NAS
    synapse assembly GO:0007416 NAS
    positive regulation of neuron projection development GO:0010976 IBA
    neurogenesis GO:0022008 NAS
    corpus callosum development GO:0022038 ISS
    neuron differentiation GO:0030182 ISS
    neuron projection development GO:0031175 ISS
    non-canonical Wnt signaling pathway GO:0035567 NAS
    chemorepulsion of dopaminergic neuron axon GO:0036518 ISS
    positive regulation of MAPK cascade GO:0043410 IDA
    skeletal system morphogenesis GO:0048705 IEA
    negative regulation of axon extension involved in axon guidance GO:0048843 IEA
    axon extension involved in axon guidance GO:0048846 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IBA
    canonical Wnt signaling pathway GO:0060070 NAS
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 TAS
    commissural neuron axon guidance GO:0071679 ISS
    positive regulation of cell proliferation in midbrain GO:1904935 ISS
    midbrain dopaminergic neuron differentiation GO:1904948 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleus GO:0005634 NAS
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISS
    membrane GO:0016020 NAS
    receptor complex GO:0043235 IBA
    presynaptic active zone membrane GO:0048787 IEA
    postsynaptic density membrane GO:0098839 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified RYK in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RYK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 RAET1E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NPEPPS 9520
Affinity Capture-MS Homo sapiens
5 PPP3CA 5530
Two-hybrid Homo sapiens
6 TMEM30A 55754
Affinity Capture-MS Homo sapiens
7 HBD 3045
Affinity Capture-MS Homo sapiens
8 PCDHGA6 56109
Affinity Capture-MS Homo sapiens
9 MFAP3  
Affinity Capture-MS Homo sapiens
10 F5 2153
Affinity Capture-MS Homo sapiens
11 PCDHGA5  
Affinity Capture-MS Homo sapiens
12 CELSR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
14 DAG1 1605
Affinity Capture-MS Homo sapiens
15 WASF2 10163
Affinity Capture-MS Homo sapiens
16 EFNB1 1947
Affinity Capture-MS Homo sapiens
17 PCDHGA11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CDH5  
Affinity Capture-MS Homo sapiens
19 HSPA13 6782
Affinity Capture-MS Homo sapiens
20 SGCE 8910
Affinity Capture-MS Homo sapiens
21 UBC 7316
Affinity Capture-MS Homo sapiens
22 ITIH2 3698
Affinity Capture-MS Homo sapiens
23 IFNGR1 3459
Affinity Capture-MS Homo sapiens
24 MILR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PRKDC 5591
Affinity Capture-MS Homo sapiens
26 VPS26A 9559
Affinity Capture-MS Homo sapiens
27 FAT3 120114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SDK1 221935
Affinity Capture-MS Homo sapiens
29 ITFG1 81533
Affinity Capture-MS Homo sapiens
30 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PTPRD 5789
Affinity Capture-MS Homo sapiens
32 CDH2 1000
Affinity Capture-MS Homo sapiens
33 DUSP14 11072
Affinity Capture-MS Homo sapiens
34 CPD 1362
Affinity Capture-MS Homo sapiens
35 DSC2 1824
Affinity Capture-MS Homo sapiens
36 NPM1 4869
Affinity Capture-MS Homo sapiens
37 FGFR4 2264
Affinity Capture-MS Homo sapiens
38 NUP93 9688
Affinity Capture-MS Homo sapiens
39 TRAP1 10131
Affinity Capture-MS Homo sapiens
40 ABI1 10006
Affinity Capture-MS Homo sapiens
41 CD276 80381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PTPRR  
Two-hybrid Homo sapiens
43 LRRC4B  
Affinity Capture-MS Homo sapiens
44 C3orf52  
Affinity Capture-MS Homo sapiens
45 DSG4 147409
Affinity Capture-MS Homo sapiens
46 NLGN2 57555
Affinity Capture-MS Homo sapiens
47 PTK7 5754
Affinity Capture-MS Homo sapiens
48 TNFSF9  
Affinity Capture-MS Homo sapiens
49 NEO1 4756
Affinity Capture-MS Homo sapiens
50 PCDH19 57526
Affinity Capture-MS Homo sapiens
51 TIMM50 92609
Affinity Capture-MS Homo sapiens
52 WASF3 10810
Affinity Capture-MS Homo sapiens
53 TMEM59 9528
Affinity Capture-MS Homo sapiens
54 TFRC 7037
Affinity Capture-MS Homo sapiens
55 PCNA 5111
Affinity Capture-MS Homo sapiens
56 MICA 100507436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 DSC3 1825
Affinity Capture-MS Homo sapiens
58 IGDCC4  
Affinity Capture-MS Homo sapiens
59 MAPK1 5594
Affinity Capture-MS Homo sapiens
60 PPM1B 5495
Two-hybrid Homo sapiens
61 ACTA2 59
Affinity Capture-MS Homo sapiens
62 SDK2 54549
Affinity Capture-MS Homo sapiens
63 PCDH1 5097
Affinity Capture-MS Homo sapiens
64 CNNM1  
Affinity Capture-MS Homo sapiens
65 NCAM1 4684
Affinity Capture-MS Homo sapiens
66 MIB1 57534
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
67 MAPK3 5595
Affinity Capture-MS Homo sapiens
68 WASF1 8936
Affinity Capture-MS Homo sapiens
69 CNNM3 26505
Affinity Capture-MS Homo sapiens
70 CEACAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 AGK 55750
Affinity Capture-MS Homo sapiens
72 PLD1 5337
Affinity Capture-MS Homo sapiens
73 DCHS1 8642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 FAT4 79633
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 APLP2 334
Affinity Capture-MS Homo sapiens
76 CSPG4 1464
Affinity Capture-MS Homo sapiens
77 PCDHGC4  
Affinity Capture-MS Homo sapiens
78 PCDHGA7 56108
Affinity Capture-MS Homo sapiens
79 TRPM7 54822
Affinity Capture-MS Homo sapiens
80 ITM2C 81618
Affinity Capture-MS Homo sapiens
81 NOMO3 408050
Affinity Capture-MS Homo sapiens
82 SLC7A6 9057
Affinity Capture-MS Homo sapiens
83 CSNK1E 1454
Affinity Capture-MS Homo sapiens
84 PLXNA2 5362
Affinity Capture-MS Homo sapiens
85 RHOT2 89941
Affinity Capture-MS Homo sapiens
86 CCBL2 56267
Affinity Capture-MS Homo sapiens
87 PCDH18 54510
Affinity Capture-MS Homo sapiens
88 ST13 6767
Affinity Capture-MS Homo sapiens
89 ITM2B 9445
Affinity Capture-MS Homo sapiens
90 ULBP3 79465
Affinity Capture-MS Homo sapiens
91 LRRC15 131578
Affinity Capture-MS Homo sapiens
92 CTNNA2 1496
Affinity Capture-MS Homo sapiens
93 BCAM 4059
Affinity Capture-MS Homo sapiens
94 ROR1 4919
Affinity Capture-MS Homo sapiens
95 ALCAM 214
Affinity Capture-MS Homo sapiens
96 PCDH20  
Affinity Capture-MS Homo sapiens
97 AIFM1 9131
Affinity Capture-MS Homo sapiens
98 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 ADGRA2 25960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 DCBLD1  
Affinity Capture-MS Homo sapiens
101 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 NCKAP1 10787
Affinity Capture-MS Homo sapiens
103 SECTM1 6398
Affinity Capture-MS Homo sapiens
104 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
105 PTH2R  
Affinity Capture-MS Homo sapiens
106 PTPRA 5786
Affinity Capture-MS Homo sapiens
107 CNTN1 1272
Affinity Capture-MS Homo sapiens
108 PGAM4 441531
Affinity Capture-MS Homo sapiens
109 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
110 OSGEP 55644
Affinity Capture-MS Homo sapiens
111 PCDHGA12  
Affinity Capture-MS Homo sapiens
112 NRP2 8828
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 NXF1 10482
Affinity Capture-RNA Homo sapiens
114 EPHA7 2045
Affinity Capture-MS Homo sapiens
115 PCDH7 5099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 KIRREL 55243
Affinity Capture-MS Homo sapiens
117 MICB  
Affinity Capture-MS Homo sapiens
118 CDHR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 PCDH10 57575
Affinity Capture-MS Homo sapiens
120 VIM 7431
Affinity Capture-MS Homo sapiens
121 SELENBP1 8991
Affinity Capture-MS Homo sapiens
122 PKP4 8502
Affinity Capture-MS Homo sapiens
123 MET 4233
Affinity Capture-MS Homo sapiens
124 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 CTNNA1 1495
Affinity Capture-MS Homo sapiens
126 KIAA0319L 79932
Affinity Capture-MS Homo sapiens
127 KIDINS220 57498
Affinity Capture-MS Homo sapiens
128 PCDHB2  
Affinity Capture-MS Homo sapiens
129 EPHB3 2049
Affinity Capture-Western Homo sapiens
130 NRP1 8829
Affinity Capture-MS Homo sapiens
131 PVRL2 5819
Affinity Capture-MS Homo sapiens
132 TMED10 10972
Affinity Capture-MS Homo sapiens
133 PARP1 142
Affinity Capture-MS Homo sapiens
134 PCDH17  
Affinity Capture-MS Homo sapiens
135 TP53 7157
Affinity Capture-MS Homo sapiens
136 PCDH9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 EPHA4 2043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 EPHB2 2048
Affinity Capture-Western Homo sapiens
139 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 CELSR1 9620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 FREM2 341640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 GPR125  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 CDON  
Affinity Capture-MS Homo sapiens
144 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
145 PODXL2 50512
Affinity Capture-MS Homo sapiens
146 EFNB3 1949
Affinity Capture-MS Homo sapiens
147 SHMT2 6472
Affinity Capture-RNA Homo sapiens
148 CDK1 983
Affinity Capture-MS Homo sapiens
149 FGFR2 2263
Affinity Capture-MS Homo sapiens
150 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
151 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
152 MOV10 4343
Affinity Capture-RNA Homo sapiens
153 PTPRS 5802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
154 CELSR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 PRTG  
Affinity Capture-MS Homo sapiens
156 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 EPHA2 1969
Affinity Capture-MS Homo sapiens
158 PTPRK 5796
Affinity Capture-MS Homo sapiens
159 XPR1 9213
Affinity Capture-MS Homo sapiens
160 ANK3  
Affinity Capture-MS Homo sapiens
161 CYFIP1 23191
Affinity Capture-MS Homo sapiens
162 LOC100507703  
Affinity Capture-MS Homo sapiens
163 EPHB4 2050
Affinity Capture-MS Homo sapiens
164 CNNM2 54805
Affinity Capture-MS Homo sapiens
165 DSG2 1829
Affinity Capture-MS Homo sapiens
166 GNB2 2783
Affinity Capture-MS Homo sapiens
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