ExoCarta: Gene summary

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Gene description for ULBP3
Gene name	UL16 binding protein 3
Gene symbol	ULBP3
Other names/aliases	RAET1N
Species	Homo sapiens
Database cross references - ULBP3
ExoCarta	ExoCarta_79465
Vesiclepedia	VP_79465
Entrez Gene	79465
HGNC	14895
MIM	605699
UniProt	Q9BZM4
ULBP3 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Gastric cancer cells	33991177

Gene ontology annotations for ULBP3

Molecular Function
	protein binding	GO:0005515	IPI
	natural killer cell lectin-like receptor binding	GO:0046703	IDA
Biological Process
	positive regulation of T cell mediated cytotoxicity	GO:0001916	IBA
	antigen processing and presentation of endogenous peptide antigen via MHC class Ib	GO:0002476	IBA
	antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent	GO:0002486	IBA
	immune response	GO:0006955	IBA
	natural killer cell activation	GO:0030101	IDA
	natural killer cell mediated cytotoxicity	GO:0042267	IDA
Subcellular Localization
	extracellular space	GO:0005615	IBA
	plasma membrane	GO:0005886	IDA
	plasma membrane	GO:0005886	TAS
	external side of plasma membrane	GO:0009897	IBA

Experiment description of studies that identified ULBP3 in sEVs

Experiment ID

363

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

364

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

365

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for ULBP3

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

VCAM1

7412

Affinity Capture-MS

Homo sapiens

SLC39A12

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DUSP22

Affinity Capture-MS

Homo sapiens

RAET1E

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FCGR1A

Affinity Capture-MS

Homo sapiens

CD200R1

131450

Affinity Capture-MS

Homo sapiens

IL13RA2

3598

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NHLRC3

387921

Affinity Capture-MS

Homo sapiens

RYK

6259

Affinity Capture-MS

Homo sapiens

BTNL9

Affinity Capture-MS

Homo sapiens

ULBP1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NRSN1

Affinity Capture-MS

Homo sapiens

FCGRT

2217

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TNFSF8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HPN

Affinity Capture-MS

Homo sapiens

PSCA

8000

Affinity Capture-MS

Homo sapiens

PTH2R

Affinity Capture-MS

Homo sapiens

CD1B

910

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TMEM30B

161291

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HCRTR2

Affinity Capture-MS

Homo sapiens

PARM1

Affinity Capture-MS

Homo sapiens

CEACAM21

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TRPV3

Affinity Capture-MS

Homo sapiens

GPRC5D

Affinity Capture-MS

Homo sapiens

IL3RA

Affinity Capture-MS

Homo sapiens

SOX2

Affinity Capture-MS

Homo sapiens

IL27RA

9466

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZDHHC23

Affinity Capture-MS

Homo sapiens

CERS2

29956

Affinity Capture-MS

Homo sapiens

GJB7

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PTP4A3

Affinity Capture-MS

Homo sapiens

CCNL2

81669

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BRICD5

Affinity Capture-MS

Homo sapiens

TMEM257

Affinity Capture-MS

Homo sapiens

IL2RA

Affinity Capture-MS

Homo sapiens

HLA-DPA1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CTLA4

Affinity Capture-MS

Homo sapiens

SLC39A5

283375

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PCDHA9

Affinity Capture-MS

Homo sapiens

GINM1

116254

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

VAMP5

10791

Affinity Capture-MS

Homo sapiens

PVR

5817

Affinity Capture-MS

Homo sapiens

ST14

6768

Affinity Capture-MS

Homo sapiens

SLAMF8

Affinity Capture-MS

Homo sapiens

ABCE1

6059

Affinity Capture-MS

Homo sapiens

SCN3B

Affinity Capture-MS

Homo sapiens

PDGFRA

5156

Affinity Capture-MS

Homo sapiens

ITFG3

83986

Affinity Capture-MS

Homo sapiens

TSPAN1

10103

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which ULBP3 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Immune System	TAS	Reactome
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here