Gene description for NHLRC3
Gene name NHL repeat containing 3
Gene symbol NHLRC3
Other names/aliases -
Species Homo sapiens
 Database cross references - NHLRC3
ExoCarta ExoCarta_387921
Vesiclepedia VP_387921
Entrez Gene 387921
HGNC 33751
UniProt Q5JS37  
 NHLRC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NHLRC3
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    azurophil granule lumen GO:0035578 TAS
 Experiment description of studies that identified NHLRC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NHLRC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FLVCR1 28982
Affinity Capture-MS Homo sapiens
2 ULBP3 79465
Affinity Capture-MS Homo sapiens
3 SLC19A1 6573
Affinity Capture-MS Homo sapiens
4 MR1  
Affinity Capture-MS Homo sapiens
5 NAAA  
Affinity Capture-MS Homo sapiens
6 CANX 821
Affinity Capture-MS Homo sapiens
7 HSD11B1 3290
Affinity Capture-MS Homo sapiens
8 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TMEM214 54867
Affinity Capture-MS Homo sapiens
10 TAZ  
Affinity Capture-MS Homo sapiens
11 EPHA4 2043
Affinity Capture-MS Homo sapiens
12 DDOST 1650
Affinity Capture-MS Homo sapiens
13 HTR3C  
Affinity Capture-MS Homo sapiens
14 DEFB121  
Affinity Capture-MS Homo sapiens
15 UGT8  
Affinity Capture-MS Homo sapiens
16 HS6ST1  
Affinity Capture-MS Homo sapiens
17 TTPAL 79183
Affinity Capture-MS Homo sapiens
18 OGFOD3 79701
Affinity Capture-MS Homo sapiens
19 OGG1  
Affinity Capture-MS Homo sapiens
20 KIRREL 55243
Affinity Capture-MS Homo sapiens
21 EXT1 2131
Affinity Capture-MS Homo sapiens
22 MPZL1 9019
Affinity Capture-MS Homo sapiens
23 DNAJB9 4189
Affinity Capture-MS Homo sapiens
24 TMEM231 79583
Affinity Capture-MS Homo sapiens
25 CLPSL1  
Affinity Capture-MS Homo sapiens
26 CPD 1362
Affinity Capture-MS Homo sapiens
27 LMF2 91289
Affinity Capture-MS Homo sapiens
28 CD276 80381
Affinity Capture-MS Homo sapiens
29 EIF2AK3  
Affinity Capture-MS Homo sapiens
30 EFNB3 1949
Affinity Capture-MS Homo sapiens
31 TNFSF9  
Affinity Capture-MS Homo sapiens
32 BTNL8  
Affinity Capture-MS Homo sapiens
33 AGRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ICAM4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NHLRC3 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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