Gene description for LMF2
Gene name lipase maturation factor 2
Gene symbol LMF2
Other names/aliases TMEM112B
TMEM153
Species Homo sapiens
 Database cross references - LMF2
ExoCarta ExoCarta_91289
Vesiclepedia VP_91289
Entrez Gene 91289
HGNC 25096
UniProt Q9BU23  
 LMF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LMF2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein maturation GO:0051604 IBA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified LMF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LMF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HFE  
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 MTCH1 23787
Proximity Label-MS Homo sapiens
4 CHRNA4  
Affinity Capture-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 ARSE 415
Affinity Capture-MS Homo sapiens
10 MFAP4 4239
Affinity Capture-MS Homo sapiens
11 IGFBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ST3GAL2 6483
Affinity Capture-MS Homo sapiens
13 SLC22A9  
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 B3GAT1  
Proximity Label-MS Homo sapiens
16 STS 412
Affinity Capture-MS Homo sapiens
17 CEACAM8 1088
Affinity Capture-MS Homo sapiens
18 KRT40  
Two-hybrid Homo sapiens
19 KIR2DS3  
Affinity Capture-MS Homo sapiens
20 CALCOCO2  
Two-hybrid Homo sapiens
21 ST14 6768
Affinity Capture-MS Homo sapiens
22 KRT31 3881
Two-hybrid Homo sapiens
23 CCDC47 57003
Affinity Capture-MS Homo sapiens
24 SSR1 6745
Proximity Label-MS Homo sapiens
25 MARCKS 4082
Proximity Label-MS Homo sapiens
26 LAMTOR1 55004
Proximity Label-MS Homo sapiens
27 KIAA1715 80856
Proximity Label-MS Homo sapiens
28 PXMP2  
Proximity Label-MS Homo sapiens
29 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ATP1B4  
Affinity Capture-MS Homo sapiens
32 EMD 2010
Proximity Label-MS Homo sapiens
33 CHRNB2  
Affinity Capture-MS Homo sapiens
34 SYT3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 RPN1 6184
Proximity Label-MS Homo sapiens
37 IL17RC 84818
Affinity Capture-MS Homo sapiens
38 GRIN2A  
Affinity Capture-MS Homo sapiens
39 UPK1A 11045
Affinity Capture-MS Homo sapiens
40 ELOVL5 60481
Proximity Label-MS Homo sapiens
41 SEC61B 10952
Proximity Label-MS Homo sapiens
42 TMPRSS13  
Affinity Capture-MS Homo sapiens
43 ZACN  
Affinity Capture-MS Homo sapiens
44 RMDN3 55177
Proximity Label-MS Homo sapiens
45 PNLDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 REEP5 7905
Proximity Label-MS Homo sapiens
47 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GRIA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 NHLRC3 387921
Affinity Capture-MS Homo sapiens
50 RPN2 6185
Proximity Label-MS Homo sapiens
51 LRRC59 55379
Proximity Label-MS Homo sapiens
52 LMNB1 4001
Proximity Label-MS Homo sapiens
53 CLPP 8192
Proximity Label-MS Homo sapiens
54 CCT6A 908
Cross-Linking-MS (XL-MS) Homo sapiens
55 HMOX2 3163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 LAMP1 3916
Proximity Label-MS Homo sapiens
57 CRELD1 78987
Affinity Capture-MS Homo sapiens
58 HSD3B7 80270
Proximity Label-MS Homo sapiens
59 CUL3 8452
Affinity Capture-MS Homo sapiens
60 STIM1 6786
Proximity Label-MS Homo sapiens
61 ARF6 382
Proximity Label-MS Homo sapiens
62 FBXO6 26270
Affinity Capture-MS Homo sapiens
63 KRTAP10-3  
Two-hybrid Homo sapiens
64 FUT3 2525
Affinity Capture-MS Homo sapiens
65 CHRNB4  
Affinity Capture-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 ATP2A1 487
Proximity Label-MS Homo sapiens
68 FBXO2 26232
Affinity Capture-MS Homo sapiens
69 A4GNT  
Affinity Capture-MS Homo sapiens
70 RAB9A 9367
Proximity Label-MS Homo sapiens
71 P2RX5 5026
Affinity Capture-MS Homo sapiens
72 ACAD9 28976
Proximity Label-MS Homo sapiens
73 DPM2 8818
Affinity Capture-MS Homo sapiens
74 MPPE1 65258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PEX3 8504
Proximity Label-MS Homo sapiens
76 AKAP1 8165
Proximity Label-MS Homo sapiens
77 SVOPL  
Affinity Capture-MS Homo sapiens
78 HTR3B  
Affinity Capture-MS Homo sapiens
79 CRLF2 64109
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
83 GABRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 SEMA7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 LRRC55  
Affinity Capture-MS Homo sapiens
86 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 SEC62 7095
Proximity Label-MS Homo sapiens
88 SLC25A51  
Proximity Label-MS Homo sapiens
89 NCEH1 57552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 RAB5C 5878
Proximity Label-MS Homo sapiens
91 CKAP4 10970
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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