Gene description for DPM2
Gene name dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit
Gene symbol DPM2
Other names/aliases CDG1U
Species Homo sapiens
 Database cross references - DPM2
ExoCarta ExoCarta_8818
Entrez Gene 8818
HGNC 3006
MIM 603564
UniProt O94777  
 DPM2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DPM2
Molecular Function
    enzyme regulator activity GO:0030234 IBA
    protein binding GO:0005515 IPI
    dolichyl-phosphate beta-D-mannosyltransferase activity GO:0004582 IDA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    GPI anchor biosynthetic process GO:0006506 IDA
    regulation of catalytic activity GO:0050790 IEA
    protein O-linked mannosylation GO:0035269 TAS
    regulation of protein stability GO:0031647 IPI
    C-terminal protein lipidation GO:0006501 TAS
    post-translational protein modification GO:0043687 TAS
    dolichol metabolic process GO:0019348 IBA
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    preassembly of GPI anchor in ER membrane GO:0016254 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    integral component of endoplasmic reticulum membrane GO:0030176 IEA
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 TAS
    dolichol-phosphate-mannose synthase complex GO:0033185 IDA
 Experiment description of studies that identified DPM2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DPM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPM1  
Co-purification Homo sapiens
2 DSCR5  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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