Gene description for DPM2
Gene name dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit
Gene symbol DPM2
Other names/aliases CDG1U
Species Homo sapiens
 Database cross references - DPM2
ExoCarta ExoCarta_8818
Vesiclepedia VP_8818
Entrez Gene 8818
HGNC 3006
MIM 603564
UniProt O94777  
 DPM2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DPM2
Molecular Function
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 IDA
    enzyme regulator activity GO:0030234 IBA
Biological Process
    GPI anchor biosynthetic process GO:0006506 IBA
    GPI anchor biosynthetic process GO:0006506 IDA
    dolichol metabolic process GO:0019348 IDA
    regulation of protein stability GO:0031647 IPI
    protein O-linked mannosylation GO:0035269 TAS
Subcellular Localization
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 IDA
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 IPI
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 TAS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    dolichol-phosphate-mannose synthase complex GO:0033185 IBA
    dolichol-phosphate-mannose synthase complex GO:0033185 IDA
    dolichol-phosphate-mannose synthase complex GO:0033185 IPI
 Experiment description of studies that identified DPM2 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DPM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POMT2  
Affinity Capture-MS Homo sapiens
2 PIGP  
Affinity Capture-Western Homo sapiens
3 FZD3  
Affinity Capture-MS Homo sapiens
4 WDR46  
Affinity Capture-MS Homo sapiens
5 PRRC2A 7916
Affinity Capture-MS Homo sapiens
6 SLC12A7 10723
Affinity Capture-MS Homo sapiens
7 DIDO1  
Affinity Capture-MS Homo sapiens
8 DPY19L4  
Affinity Capture-MS Homo sapiens
9 MPRIP 23164
Affinity Capture-MS Homo sapiens
10 SVIL 6840
Affinity Capture-MS Homo sapiens
11 PIGO  
Affinity Capture-MS Homo sapiens
12 TMEM214 54867
Affinity Capture-MS Homo sapiens
13 UGT3A2  
Affinity Capture-MS Homo sapiens
14 PHF3  
Affinity Capture-MS Homo sapiens
15 MYH14 79784
Affinity Capture-MS Homo sapiens
16 RBMXL1  
Affinity Capture-MS Homo sapiens
17 TUSC3 7991
Affinity Capture-MS Homo sapiens
18 ZC3H4 23211
Affinity Capture-MS Homo sapiens
19 MYO5A 4644
Affinity Capture-MS Homo sapiens
20 MYO5C 55930
Affinity Capture-MS Homo sapiens
21 ABCC10  
Affinity Capture-MS Homo sapiens
22 UTP11L  
Affinity Capture-MS Homo sapiens
23 CCPG1 9236
Affinity Capture-MS Homo sapiens
24 EEPD1 80820
Affinity Capture-MS Homo sapiens
25 SFSWAP  
Affinity Capture-MS Homo sapiens
26 DPM1 8813
Co-purification Homo sapiens
27 SAFB2 9667
Affinity Capture-MS Homo sapiens
28 MYH9 4627
Affinity Capture-MS Homo sapiens
29 MYO5B 4645
Affinity Capture-MS Homo sapiens
30 PRPF38B 55119
Affinity Capture-MS Homo sapiens
31 DNAJB9 4189
Affinity Capture-MS Homo sapiens
32 RAI14 26064
Affinity Capture-MS Homo sapiens
33 VKORC1 79001
Two-hybrid Homo sapiens
34 LMF2 91289
Affinity Capture-MS Homo sapiens
35 MYH10 4628
Affinity Capture-MS Homo sapiens
36 ZNF512B  
Affinity Capture-MS Homo sapiens
37 CACTIN  
Affinity Capture-MS Homo sapiens
38 ARGLU1  
Affinity Capture-MS Homo sapiens
39 UGT8  
Affinity Capture-MS Homo sapiens
40 SLC12A4 6560
Affinity Capture-MS Homo sapiens
41 GLRB  
Affinity Capture-MS Homo sapiens
42 SLC12A6 9990
Affinity Capture-MS Homo sapiens
43 HELLS 3070
Affinity Capture-MS Homo sapiens
44 ALG9 79796
Affinity Capture-MS Homo sapiens
45 SLTM 79811
Affinity Capture-MS Homo sapiens
46 LRCH3 84859
Affinity Capture-MS Homo sapiens
47 FZD2 2535
Affinity Capture-MS Homo sapiens
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