Gene description for TUSC3
Gene name tumor suppressor candidate 3
Gene symbol TUSC3
Other names/aliases D8S1992
M33
MRT22
MRT7
N33
OST3A
Species Homo sapiens
 Database cross references - TUSC3
ExoCarta ExoCarta_7991
Vesiclepedia VP_7991
Entrez Gene 7991
HGNC 30242
MIM 601385
UniProt Q13454  
 TUSC3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TUSC3
Molecular Function
    magnesium ion transmembrane transporter activity GO:0015095 IMP
    magnesium ion transmembrane transporter activity GO:0015095 TAS
Biological Process
    protein N-linked glycosylation GO:0006487 NAS
    magnesium ion transport GO:0015693 IGI
    magnesium ion transport GO:0015693 IGI
    magnesium ion transport GO:0015693 IMP
    protein N-linked glycosylation via asparagine GO:0018279 IBA
    protein N-linked glycosylation via asparagine GO:0018279 IMP
    cognition GO:0050890 IMP
    transmembrane transport GO:0055085 TAS
    magnesium ion transmembrane transport GO:1903830 IEA
Subcellular Localization
    mitochondrion GO:0005739 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    oligosaccharyltransferase complex GO:0008250 IBA
    oligosaccharyltransferase complex GO:0008250 IDA
 Experiment description of studies that identified TUSC3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TUSC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACC1 6867
Affinity Capture-MS Homo sapiens
2 NKAIN1  
Affinity Capture-MS Homo sapiens
3 HFE  
Affinity Capture-MS Homo sapiens
4 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHRNA4  
Affinity Capture-MS Homo sapiens
6 TTYH1  
Affinity Capture-MS Homo sapiens
7 SIDT2 51092
Affinity Capture-MS Homo sapiens
8 RNF185  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GABRA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HPN  
Affinity Capture-MS Homo sapiens
11 STXBP1 6812
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PPP1CA 5499
Two-hybrid Homo sapiens
14 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DAD1 1603
Affinity Capture-MS Homo sapiens
17 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
18 FLOT2 2319
Affinity Capture-MS Homo sapiens
19 LIN54  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GGT7 2686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FBXO6 26270
Affinity Capture-MS Homo sapiens
22 NDRG3 57446
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MAP2K1 5604
Synthetic Lethality Homo sapiens
25 TUBA1A 7846
Synthetic Lethality Homo sapiens
26 FAM122B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 OST4  
Affinity Capture-MS Homo sapiens
28 CLEC12B  
Affinity Capture-MS Homo sapiens
29 CHRNB2  
Affinity Capture-MS Homo sapiens
30 MLEC 9761
Affinity Capture-MS Homo sapiens
31 CHRNB4  
Affinity Capture-MS Homo sapiens
32 EVI2B 2124
Affinity Capture-MS Homo sapiens
33 STXBP2 6813
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 EIF2AK3  
Affinity Capture-MS Homo sapiens
35 CHEK1  
Synthetic Lethality Homo sapiens
36 SLC28A2  
Affinity Capture-MS Homo sapiens
37 TMED7 51014
Affinity Capture-MS Homo sapiens
38 STT3B 201595
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DPM2 8818
Affinity Capture-MS Homo sapiens
40 RANBP1 5902
Affinity Capture-MS Homo sapiens
41 BMI1  
Reconstituted Complex Homo sapiens
42 CHRNE  
Affinity Capture-MS Homo sapiens
43 ST14 6768
Affinity Capture-MS Homo sapiens
44 UPK1A 11045
Affinity Capture-MS Homo sapiens
45 DDOST 1650
Affinity Capture-MS Homo sapiens
46 GABRD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RP2 6102
Affinity Capture-MS Homo sapiens
48 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 LIN52  
Affinity Capture-MS Homo sapiens
50 RPN2 6185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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