Gene description for SLC12A6
Gene name solute carrier family 12 (potassium/chloride transporter), member 6
Gene symbol SLC12A6
Other names/aliases ACCPN
KCC3
KCC3A
KCC3B
Species Homo sapiens
 Database cross references - SLC12A6
ExoCarta ExoCarta_9990
Vesiclepedia VP_9990
Entrez Gene 9990
HGNC 10914
MIM 604878
UniProt Q9UHW9  
 SLC12A6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC12A6
Molecular Function
    potassium ion transmembrane transporter activity GO:0015079 IDA
    potassium:chloride symporter activity GO:0015379 IBA
    potassium:chloride symporter activity GO:0015379 IDA
    potassium:chloride symporter activity GO:0015379 TAS
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 NAS
    monoatomic ion transport GO:0006811 IDA
    monoatomic ion transport GO:0006811 TAS
    cell volume homeostasis GO:0006884 IBA
    cell volume homeostasis GO:0006884 IDA
    chemical synaptic transmission GO:0007268 IBA
    chloride ion homeostasis GO:0055064 IBA
    chloride ion homeostasis GO:0055064 ISS
    potassium ion homeostasis GO:0055075 IBA
    potassium ion homeostasis GO:0055075 ISS
    cellular response to glucose stimulus GO:0071333 IEA
    cellular hypotonic response GO:0071476 IMP
    cellular hypotonic salinity response GO:0071477 IDA
    potassium ion transmembrane transport GO:0071805 IDA
    chloride transmembrane transport GO:1902476 IBA
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 NAS
    basolateral plasma membrane GO:0016323 ISS
    axon GO:0030424 IEA
    synapse GO:0045202 IEA
 Experiment description of studies that identified SLC12A6 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC12A6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NKAIN1  
Affinity Capture-MS Homo sapiens
2 OR10H1  
Affinity Capture-MS Homo sapiens
3 RXFP1  
Affinity Capture-MS Homo sapiens
4 OR10H2  
Affinity Capture-MS Homo sapiens
5 LGALS9C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RAB35 11021
Affinity Capture-MS Homo sapiens
7 STK39 27347
Affinity Capture-MS Homo sapiens
8 LGALS1 3956
Affinity Capture-MS Homo sapiens
9 HSPA4L 22824
Affinity Capture-MS Homo sapiens
10 STX4 6810
Proximity Label-MS Homo sapiens
11 PDE2A  
Affinity Capture-MS Homo sapiens
12 MS4A15  
Affinity Capture-MS Homo sapiens
13 RNF5  
Affinity Capture-MS Homo sapiens
14 PTCHD3  
Affinity Capture-MS Homo sapiens
15 RAB3B 5865
Proximity Label-MS Homo sapiens
16 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
17 AGPAT2  
Affinity Capture-MS Homo sapiens
18 PIGH  
Affinity Capture-MS Homo sapiens
19 DNAJB1 3337
Affinity Capture-MS Homo sapiens
20 STIP1 10963
Affinity Capture-MS Homo sapiens
21 RHOB 388
Proximity Label-MS Homo sapiens
22 C3orf52  
Affinity Capture-MS Homo sapiens
23 STX6 10228
Proximity Label-MS Homo sapiens
24 RGS9BP  
Affinity Capture-MS Homo sapiens
25 DNAJC7 7266
Affinity Capture-MS Homo sapiens
26 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
27 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 RAB6B 51560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SLC12A7 10723
Affinity Capture-MS Homo sapiens
30 CLGN 1047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ATP6AP1 537
Affinity Capture-MS Homo sapiens
32 LAMTOR1 55004
Proximity Label-MS Homo sapiens
33 BAG5 9529
Affinity Capture-MS Homo sapiens
34 CNNM4 26504
Affinity Capture-MS Homo sapiens
35 BAG2 9532
Affinity Capture-MS Homo sapiens
36 RAB14 51552
Affinity Capture-MS Homo sapiens
37 OR2A2  
Affinity Capture-MS Homo sapiens
38 AMFR 267
Affinity Capture-MS Homo sapiens
39 STOM 2040
Affinity Capture-MS Homo sapiens
40 JAGN1 84522
Affinity Capture-MS Homo sapiens
41 LYN 4067
Proximity Label-MS Homo sapiens
42 ILVBL 10994
Affinity Capture-MS Homo sapiens
43 RAB4A 5867
Proximity Label-MS Homo sapiens
44 RAB9B 51209
Affinity Capture-MS Homo sapiens
45 UBAC2 337867
Affinity Capture-MS Homo sapiens
46 PCDHGC4  
Affinity Capture-MS Homo sapiens
47 SCAMP2 10066
Affinity Capture-MS Homo sapiens
48 SLC4A8 9498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DNAJC16  
Affinity Capture-MS Homo sapiens
50 WTH3DI 150786
Affinity Capture-MS Homo sapiens
51 VAV2 7410
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
52 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
53 DNAJB5  
Affinity Capture-MS Homo sapiens
54 NMUR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CLRN2  
Affinity Capture-MS Homo sapiens
56 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 BAG3 9531
Affinity Capture-MS Homo sapiens
58 SLC17A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RAB6A 5870
Affinity Capture-MS Homo sapiens
60 TMEM30A 55754
Affinity Capture-MS Homo sapiens
61 TTYH1  
Affinity Capture-MS Homo sapiens
62 CANX 821
Affinity Capture-MS Homo sapiens
63 DNAJB12 54788
Affinity Capture-MS Homo sapiens
64 AHCYL1 10768
Affinity Capture-MS Homo sapiens
65 RAB5A 5868
Proximity Label-MS Homo sapiens
66 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 SLC41A3 54946
Affinity Capture-MS Homo sapiens
68 ZDHHC12  
Affinity Capture-MS Homo sapiens
69 SCAMP1 9522
Affinity Capture-MS Homo sapiens
70 LGALS9B 284194
Affinity Capture-MS Homo sapiens
71 OR10S1  
Affinity Capture-MS Homo sapiens
72 KCNMB3  
Affinity Capture-MS Homo sapiens
73 TMEM169  
Affinity Capture-MS Homo sapiens
74 FDFT1 2222
Affinity Capture-MS Homo sapiens
75 ACSL3 2181
Affinity Capture-MS Homo sapiens
76 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 ATP1B1 481
Affinity Capture-MS Homo sapiens
78 DPM2 8818
Affinity Capture-MS Homo sapiens
79 SLC12A6 9990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 NRN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
82 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SLC27A3 11000
Affinity Capture-MS Homo sapiens
84 STUB1 10273
Affinity Capture-MS Homo sapiens
85 RAB39A 54734
Affinity Capture-MS Homo sapiens
86 TMEM9 252839
Affinity Capture-MS Homo sapiens
87 SLC12A4 6560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 FAF2 23197
Affinity Capture-MS Homo sapiens
89 ERLIN1 10613
Affinity Capture-MS Homo sapiens
90 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CDC37 11140
Affinity Capture-MS Homo sapiens
92 CAV1 857
Proximity Label-MS Homo sapiens
93 OR2G6  
Affinity Capture-MS Homo sapiens
94 DDX58 23586
Affinity Capture-RNA Homo sapiens
95 AUP1 550
Affinity Capture-MS Homo sapiens
96 BSG 682
Affinity Capture-MS Homo sapiens
97 RAB2A 5862
Proximity Label-MS Homo sapiens
98 SEC62 7095
Affinity Capture-MS Homo sapiens
99 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 RAB7A 7879
Proximity Label-MS Homo sapiens
101 TMC4  
Affinity Capture-MS Homo sapiens
102 FAM189A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 EPHA2 1969
Proximity Label-MS Homo sapiens
104 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
105 MARCH4  
Affinity Capture-MS Homo sapiens
106 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
107 KRAS 3845
Proximity Label-MS Homo sapiens
108 RAB5C 5878
Proximity Label-MS Homo sapiens
109 THEM6  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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