Gene description for RAB9B
Gene name RAB9B, member RAS oncogene family
Gene symbol RAB9B
Other names/aliases RAB9L
Rab-9L
Species Homo sapiens
 Database cross references - RAB9B
ExoCarta ExoCarta_51209
Vesiclepedia VP_51209
Entrez Gene 51209
HGNC 14090
MIM 300285
UniProt Q9NP90  
 RAB9B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RAB9B
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GDP binding GO:0019003 IDA
    GTP binding GO:0005525 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    metabolic process GO:0008152 IBA
    Rab protein signal transduction GO:0032482 IBA
    phagosome-lysosome fusion GO:0090385 IBA
    retrograde transport, endosome to Golgi GO:0042147 IBA
Subcellular Localization
    late endosome GO:0005770 IBA
    plasma membrane GO:0005886 IEA
    phagocytic vesicle GO:0045335 IDA
    lysosome GO:0005764 IBA
    vacuolar membrane GO:0005774 IBA
    phagocytic vesicle membrane GO:0030670 IEA
 Experiment description of studies that identified RAB9B in exosomes
1
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
EM
EV Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
EV Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAB9B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RAB9B is involved
No pathways found





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