Gene description for FDFT1
Gene name farnesyl-diphosphate farnesyltransferase 1
Gene symbol FDFT1
Other names/aliases DGPT
ERG9
SQS
SS
Species Homo sapiens
 Database cross references - FDFT1
ExoCarta ExoCarta_2222
Entrez Gene 2222
HGNC 3629
MIM 184420
UniProt P37268  
 FDFT1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for FDFT1
Molecular Function
    squalene synthase activity GO:0051996 IEA
    oxidoreductase activity GO:0016491 IEA
    farnesyl-diphosphate farnesyltransferase activity GO:0004310 TAS
Biological Process
    steroid biosynthetic process GO:0006694 TAS
    cellular lipid metabolic process GO:0044255 TAS
    farnesyl diphosphate metabolic process GO:0045338 IEA
    oxidation-reduction process GO:0055114 IEA
    cholesterol biosynthetic process GO:0006695 TAS
    small molecule metabolic process GO:0044281 TAS
    isoprenoid biosynthetic process GO:0008299 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified FDFT1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FDFT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FDFT1 2222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 3110032G18Rik  
Two-hybrid Mus musculus
3 PSTPIP1 9051
Affinity Capture-MS Homo sapiens
4 TMEM43 79188
Two-hybrid Homo sapiens
5 RUVBL1 8607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SMNDC1  
Affinity Capture-MS Homo sapiens
7 SHPRH  
Two-hybrid Homo sapiens
8 ARL6IP1 23204
Two-hybrid Homo sapiens
9 ANXA5 308
Two-hybrid Homo sapiens
10 RUVBL2 10856
Two-hybrid Homo sapiens
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