Gene description for GDPD5
Gene name glycerophosphodiester phosphodiesterase domain containing 5
Gene symbol GDPD5
Other names/aliases GDE2
PP1665
Species Homo sapiens
 Database cross references - GDPD5
ExoCarta ExoCarta_81544
Vesiclepedia VP_81544
Entrez Gene 81544
HGNC 28804
MIM 609632
UniProt Q8WTR4  
 GDPD5 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GDPD5
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IEA
    protein binding GO:0005515 IPI
    glycerophosphodiester phosphodiesterase activity GO:0008889 IBA
    glycerophosphocholine phosphodiesterase activity GO:0047389 IEA
Biological Process
    lipid metabolic process GO:0006629 IEA
    nervous system development GO:0007399 IEA
    positive regulation of neuron differentiation GO:0045666 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    endomembrane system GO:0012505 IEA
    growth cone GO:0030426 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified GDPD5 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GDPD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
2 PRKD2 25865
Affinity Capture-MS Homo sapiens
3 TRIP6 7205
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 LYPD3 27076
Affinity Capture-MS Homo sapiens
5 KLHL15  
Affinity Capture-MS Homo sapiens
6 GPRC5C 55890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NDUFS4 4724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 STX16 8675
Affinity Capture-MS Homo sapiens
9 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 C1orf43 25912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SELT 51714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ZDHHC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HSPA8 3312
Affinity Capture-MS Homo sapiens
14 RUVBL2 10856
Affinity Capture-MS Homo sapiens
15 TM9SF3 56889
Affinity Capture-MS Homo sapiens
16 GOLIM4 27333
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GPR89B 51463
Affinity Capture-MS Homo sapiens
18 ADCY6 112
Affinity Capture-MS Homo sapiens
19 TMEM205 374882
Affinity Capture-MS Homo sapiens
20 TYW1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 GPD2 2820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ROBO1 6091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PLSCR1 5359
Two-hybrid Homo sapiens
24 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 KCNJ11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MARC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 AARSD1 80755
Affinity Capture-MS Homo sapiens
28 SGPP1 81537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FDFT1 2222
Affinity Capture-MS Homo sapiens
30 PRDM5  
Affinity Capture-MS Homo sapiens
31 BAG5 9529
Affinity Capture-MS Homo sapiens
32 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RETSAT 54884
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TEX264 51368
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KRTAP26-1  
Two-hybrid Homo sapiens
36 NDC1 55706
Affinity Capture-MS Homo sapiens
37 HSPA2 3306
Affinity Capture-MS Homo sapiens
38 LEMD2 221496
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ZDHHC18 84243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 STX8 9482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PEX5 5830
Two-hybrid Homo sapiens
45 TMEM55A 55529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FAM213A 84293
Affinity Capture-MS Homo sapiens
47 CHSY3 337876
Affinity Capture-MS Homo sapiens
48 THEM6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 GET4 51608
Affinity Capture-MS Homo sapiens
50 NDUFS6  
Affinity Capture-MS Homo sapiens
51 PIGQ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SCAMP4 113178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GDPD5 is involved
PathwayEvidenceSource
Glycerophospholipid catabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Phospholipid metabolism IEA Reactome
PI Metabolism IEA Reactome





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