Gene description for RETSAT
Gene name retinol saturase (all-trans-retinol 13,14-reductase)
Gene symbol RETSAT
Other names/aliases -
Species Homo sapiens
 Database cross references - RETSAT
ExoCarta ExoCarta_54884
Vesiclepedia VP_54884
Entrez Gene 54884
HGNC 25991
UniProt Q6NUM9  
 RETSAT identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RETSAT
Molecular Function
    all-trans-retinol 13,14-reductase activity GO:0051786 IBA
    all-trans-retinol 13,14-reductase activity GO:0051786 ISS
Biological Process
    retinol metabolic process GO:0042572 IBA
    retinol metabolic process GO:0042572 ISS
Subcellular Localization
    nuclear outer membrane GO:0005640 ISS
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 ISS
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 ISS
 Experiment description of studies that identified RETSAT in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RETSAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM116  
Affinity Capture-MS Homo sapiens
2 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ACOT7 11332
Affinity Capture-MS Homo sapiens
4 CYP26B1  
Affinity Capture-MS Homo sapiens
5 KCNS3 3790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SRPRB 58477
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 NKAIN1  
Affinity Capture-MS Homo sapiens
9 IL17RB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ICOSLG  
Affinity Capture-MS Homo sapiens
11 LGR4 55366
Affinity Capture-MS Homo sapiens
12 PTH1R  
Affinity Capture-MS Homo sapiens
13 CXCR4 7852
Affinity Capture-MS Homo sapiens
14 FAM20C 56975
Affinity Capture-MS Homo sapiens
15 TSPAN15 23555
Affinity Capture-MS Homo sapiens
16 RXFP1  
Affinity Capture-MS Homo sapiens
17 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
18 PCDHGA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 METTL7A 25840
Proximity Label-MS Homo sapiens
20 HSPA8 3312
Affinity Capture-MS Homo sapiens
21 FFAR1  
Affinity Capture-MS Homo sapiens
22 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
23 TOLLIP 54472
Proximity Label-MS Homo sapiens
24 HCST  
Affinity Capture-MS Homo sapiens
25 TMEM68  
Affinity Capture-MS Homo sapiens
26 ZDHHC12  
Affinity Capture-MS Homo sapiens
27 LAMP1 3916
Affinity Capture-MS Homo sapiens
28 PIGH  
Affinity Capture-MS Homo sapiens
29 FAS 355
Proximity Label-MS Homo sapiens
30 TMPRSS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TMEM9B 56674
Affinity Capture-MS Homo sapiens
33 IL9R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 FAM57A  
Affinity Capture-MS Homo sapiens
35 IL22RA1 58985
Affinity Capture-MS Homo sapiens
36 CD6 923
Affinity Capture-MS Homo sapiens
37 HSPA1L 3305
Affinity Capture-MS Homo sapiens
38 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TSPAN17 26262
Affinity Capture-MS Homo sapiens
40 IL17RA 23765
Affinity Capture-MS Homo sapiens
41 GPR182  
Affinity Capture-MS Homo sapiens
42 EVA1B 55194
Affinity Capture-MS Homo sapiens
43 PCDHA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 FAM63B 54629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 C9orf91 203197
Affinity Capture-MS Homo sapiens
46 PTGER3  
Affinity Capture-MS Homo sapiens
47 APP 351
Reconstituted Complex Homo sapiens
48 AMHR2  
Affinity Capture-MS Homo sapiens
49 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MPL 4352
Affinity Capture-MS Homo sapiens
51 PCDHGB2  
Affinity Capture-MS Homo sapiens
52 MARCH4  
Affinity Capture-MS Homo sapiens
53 C3orf52  
Affinity Capture-MS Homo sapiens
54 TECTB  
Affinity Capture-MS Homo sapiens
55 PCDHGC4  
Affinity Capture-MS Homo sapiens
56 HSPA2 3306
Affinity Capture-MS Homo sapiens
57 GDPD5 81544
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 LMNA 4000
Proximity Label-MS Homo sapiens
59 RAB7A 7879
Proximity Label-MS Homo sapiens
60 MLXIPL  
Affinity Capture-MS Homo sapiens
61 LRRTM1 347730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 IQCF1  
Affinity Capture-MS Homo sapiens
63 MCOLN3  
Affinity Capture-MS Homo sapiens
64 PTPRN  
Affinity Capture-MS Homo sapiens
65 TGFA  
Affinity Capture-MS Homo sapiens
66 PCDHB16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 CDHR5 53841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TREML2 79865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 KCNA2  
Affinity Capture-MS Homo sapiens
70 PEX7  
Affinity Capture-MS Homo sapiens
71 SLC31A1 1317
Affinity Capture-MS Homo sapiens
72 PCDHB3  
Affinity Capture-MS Homo sapiens
73 XKR6  
Affinity Capture-MS Homo sapiens
74 EFNB2 1948
Affinity Capture-MS Homo sapiens
75 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SCAP  
Affinity Capture-MS Homo sapiens
78 CHRNA1  
Affinity Capture-MS Homo sapiens
79 KCNK16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 TTYH1  
Affinity Capture-MS Homo sapiens
81 PCDHB7  
Affinity Capture-MS Homo sapiens
82 CHRND  
Affinity Capture-MS Homo sapiens
83 CCDC47 57003
Affinity Capture-MS Homo sapiens
84 CLRN2  
Affinity Capture-MS Homo sapiens
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