Gene description for MPL
Gene name MPL proto-oncogene, thrombopoietin receptor
Gene symbol MPL
Other names/aliases C-MPL
CD110
MPLV
THCYT2
TPOR
Species Homo sapiens
 Database cross references - MPL
ExoCarta ExoCarta_4352
Entrez Gene 4352
HGNC 7217
MIM 159530
UniProt P40238  
 MPL identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MPL
Molecular Function
    protein binding GO:0005515 IPI
    thrombopoietin receptor activity GO:0038164 IMP
    transmembrane signaling receptor activity GO:0004888 TAS
Biological Process
    platelet activation GO:0030168 TAS
    blood coagulation GO:0007596 TAS
    thrombopoietin-mediated signaling pathway GO:0038163 IMP
    regulation of chemokine production GO:0032642 IEA
    cell proliferation GO:0008283 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    homeostasis of number of cells GO:0048872 IEA
Subcellular Localization
    cell surface GO:0009986 IDA
    Golgi apparatus GO:0005794 IDA
    integral component of plasma membrane GO:0005887 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified MPL in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MPL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THPO  
Invivo Homo sapiens
2 ATXN2L 11273
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 SOCS1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MPL is involved
PathwayEvidenceSource
Platelet Aggregation (Plug Formation) TAS Reactome





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