Gene description for ROBO1
Gene name roundabout, axon guidance receptor, homolog 1 (Drosophila)
Gene symbol ROBO1
Other names/aliases DUTT1
SAX3
Species Homo sapiens
 Database cross references - ROBO1
ExoCarta ExoCarta_6091
Vesiclepedia VP_6091
Entrez Gene 6091
HGNC 10249
MIM 602430
UniProt Q9Y6N7  
 ROBO1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ROBO1
Molecular Function
    protein binding GO:0005515 IPI
    axon guidance receptor activity GO:0008046 IDA
    LRR domain binding GO:0030275 IPI
    identical protein binding GO:0042802 IDA
Biological Process
    cell migration involved in sprouting angiogenesis GO:0002042 IMP
    heart induction GO:0003129 ISS
    outflow tract septum morphogenesis GO:0003148 ISS
    aortic valve morphogenesis GO:0003180 ISS
    pulmonary valve morphogenesis GO:0003184 ISS
    endocardial cushion formation GO:0003272 ISS
    cell adhesion GO:0007155 TAS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IDA
    nervous system development GO:0007399 TAS
    positive regulation of gene expression GO:0010628 ISS
    negative regulation of gene expression GO:0010629 ISS
    axon midline choice point recognition GO:0016199 ISS
    chemorepulsion involved in postnatal olfactory bulb interneuron migration GO:0021836 IDA
    negative regulation of cell migration GO:0030336 IMP
    positive regulation of vascular endothelial growth factor receptor signaling pathway GO:0030949 IMP
    negative regulation of mammary gland epithelial cell proliferation GO:0033600 IMP
    positive regulation of Rho protein signal transduction GO:0035025 IMP
    Roundabout signaling pathway GO:0035385 IMP
    aorta development GO:0035904 ISS
    positive regulation of MAP kinase activity GO:0043406 IMP
    positive regulation of Notch signaling pathway GO:0045747 ISS
    positive regulation of axonogenesis GO:0050772 IDA
    negative regulation of negative chemotaxis GO:0050925 IDA
    ventricular septum morphogenesis GO:0060412 ISS
    negative regulation of chemokine-mediated signaling pathway GO:0070100 IMP
    positive regulation of vascular endothelial growth factor signaling pathway GO:1900748 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    axon GO:0030424 IEA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
 Experiment description of studies that identified ROBO1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ROBO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UNG  
Co-fractionation Homo sapiens
2 SRGAP1 57522
Affinity Capture-MS Homo sapiens
3 FBXO2 26232
Affinity Capture-MS Homo sapiens
4 PHLPP1  
Proximity Label-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 RAB35 11021
Proximity Label-MS Homo sapiens
7 EBAG9 9166
Proximity Label-MS Homo sapiens
8 IL31  
Affinity Capture-MS Homo sapiens
9 OCLN 100506658
Proximity Label-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 RAB5A 5868
Proximity Label-MS Homo sapiens
13 GJA1 2697
Proximity Label-MS Homo sapiens
14 PELI2 57161
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 SH2D1B  
Affinity Capture-MS Homo sapiens
16 CMKLR1 1240
Affinity Capture-MS Homo sapiens
17 SRGAP3  
Affinity Capture-MS Homo sapiens
18 TUBB3 10381
Two-hybrid Homo sapiens
19 CLEC4A  
Affinity Capture-MS Homo sapiens
20 FDXR 2232
Co-fractionation Homo sapiens
21 MAPK12  
Co-fractionation Homo sapiens
22 ARF6 382
Proximity Label-MS Homo sapiens
23 STX4 6810
Proximity Label-MS Homo sapiens
24 C11orf52 91894
Proximity Label-MS Homo sapiens
25 LYN 4067
Proximity Label-MS Homo sapiens
26 MCAM 4162
Proximity Label-MS Homo sapiens
27 MPL 4352
Affinity Capture-MS Homo sapiens
28 CDK2 1017
Affinity Capture-MS Homo sapiens
29 KCNE3  
Affinity Capture-MS Homo sapiens
30 DNAJC5 80331
Proximity Label-MS Homo sapiens
31 B3GAT1  
Proximity Label-MS Homo sapiens
32 MKRN2 23609
Affinity Capture-RNA Homo sapiens
33 PARD6B 84612
Affinity Capture-MS Homo sapiens
34 CTLA4  
Affinity Capture-MS Homo sapiens
35 GDPD5 81544
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ARPP19 10776
Co-fractionation Homo sapiens
37 MAGEB6  
Two-hybrid Homo sapiens
38 RAB11A 8766
Proximity Label-MS Homo sapiens
39 EPHA2 1969
Proximity Label-MS Homo sapiens
40 STX6 10228
Proximity Label-MS Homo sapiens
41 KRAS 3845
Proximity Label-MS Homo sapiens
42 RHOB 388
Proximity Label-MS Homo sapiens
43 MAPK14 1432
Two-hybrid Homo sapiens
44 PRSS21  
Affinity Capture-MS Homo sapiens
45 CAV1 857
Proximity Label-MS Homo sapiens
46 CTDSPL 10217
Proximity Label-MS Homo sapiens
47 USP33  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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