Gene description for MAPK14
Gene name mitogen-activated protein kinase 14
Gene symbol MAPK14
Other names/aliases CSBP
CSBP1
CSBP2
CSPB1
EXIP
Mxi2
PRKM14
PRKM15
RK
SAPK2A
p38
p38ALPHA
Species Homo sapiens
 Database cross references - MAPK14
ExoCarta ExoCarta_1432
Vesiclepedia VP_1432
Entrez Gene 1432
HGNC 6876
MIM 600289
UniProt Q16539  
 MAPK14 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for MAPK14
Molecular Function
    ATP binding GO:0005524 IEA
    MAP kinase kinase activity GO:0004708 TAS
    protein binding GO:0005515 IPI
    MAP kinase activity GO:0004707 IDA
    NFAT protein binding GO:0051525 ISS
    protein serine/threonine kinase activity GO:0004674 TAS
Biological Process
    stress-activated MAPK cascade GO:0051403 TAS
    angiogenesis GO:0001525 IEA
    innate immune response GO:0045087 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    signal transduction GO:0007165 TAS
    cellular response to ionizing radiation GO:0071479 IMP
    peptidyl-serine phosphorylation GO:0018105 ISS
    p38MAPK cascade GO:0038066 ISS
    intracellular signal transduction GO:0035556 IDA
    gene expression GO:0010467 TAS
    positive regulation of myotube differentiation GO:0010831 ISS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    platelet activation GO:0030168 TAS
    cell morphogenesis GO:0000902 IEA
    positive regulation of myoblast differentiation GO:0045663 ISS
    blood coagulation GO:0007596 TAS
    muscle cell differentiation GO:0042692 TAS
    positive regulation of reactive oxygen species metabolic process GO:2000379 IMP
    apoptotic process GO:0006915 IEA
    myoblast differentiation involved in skeletal muscle regeneration GO:0014835 IEA
    skeletal muscle tissue development GO:0007519 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    mitochondrion organization GO:0007005 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    response to muramyl dipeptide GO:0032495 IEA
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 TAS
    positive regulation of myoblast fusion GO:1901741 ISS
    cellular response to lipopolysaccharide GO:0071222 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    activation of MAPK activity GO:0000187 TAS
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    positive regulation of protein import into nucleus GO:0042307 IEA
    positive regulation of brown fat cell differentiation GO:0090336 IEA
    stress-induced premature senescence GO:0090400 IMP
    chemotaxis GO:0006935 TAS
    regulation of cytokine production involved in inflammatory response GO:1900015 IDA
    striated muscle cell differentiation GO:0051146 IEA
    signal transduction in response to DNA damage GO:0042770 IMP
    Ras protein signal transduction GO:0007265 TAS
    glucose metabolic process GO:0006006 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    DNA damage checkpoint GO:0000077 IEA
    positive regulation of muscle cell differentiation GO:0051149 TAS
    positive regulation of erythrocyte differentiation GO:0045648 IEA
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    organelle organization GO:0006996 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    cartilage condensation GO:0001502 IEA
    positive regulation of cyclase activity GO:0031281 IMP
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    fatty acid oxidation GO:0019395 IEA
    3'-UTR-mediated mRNA stabilization GO:0070935 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    transmembrane receptor protein serine/threonine kinase signaling pathway GO:0007178 IEA
    chondrocyte differentiation GO:0002062 IEA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    transcription, DNA-templated GO:0006351 IEA
    osteoclast differentiation GO:0030316 ISS
    movement of cell or subcellular component GO:0006928 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 IEA
    extracellular exosome GO:0070062 IDA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified MAPK14 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 191
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD81|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPK14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT18 3875
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 CDC25A  
Reconstituted Complex Homo sapiens
3 MAPKAPK2 9261
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
4 MAP2K6 5608
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 DUSP1  
Affinity Capture-Western Homo sapiens
6 GMFB 2764
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 DUSP10 11221
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 GRB2 2885
Affinity Capture-MS Homo sapiens
9 HTRA2 27429
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 MAP2K4 6416
Invitro Homo sapiens
11 DYRK1B  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 MKNK1  
Invitro Homo sapiens
13 MAPK1 5594
Affinity Capture-Western Homo sapiens
14 TAB1 10454
Invitro Homo sapiens
15 STK39 27347
Affinity Capture-Western Homo sapiens
16 MAPK3 5595
Affinity Capture-Western Homo sapiens
17 RPS6KA4 8986
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
18 KARS 3735
Two-hybrid Homo sapiens
19 CREB1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
20 DUSP16  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 MARS 4141
Invitro Homo sapiens
22 CSNK2A1 1457
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 DUSP6 1848
Affinity Capture-Western Homo sapiens
24 MAP2K3 5606
Affinity Capture-MS Homo sapiens
25 ATF2  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
26 PTPN7 5778
Affinity Capture-MS Homo sapiens
27 MAPKAPK3 7867
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
28 CDC25C  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
29 DDIT3 1649
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
30 MEF2A  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
31 FUBP1 8880
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 EGFR 1956
Biochemical Activity Homo sapiens
33 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
34 SPAG9 9043
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
35 DUSP4 1846
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
36 ZFP36L1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
37 PML 5371
Affinity Capture-MS Homo sapiens
38 MAPK14 1432
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 AKT1 207
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
40 MEF2C  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
41 Rps6kb1  
Reconstituted Complex Mus musculus
42 SLC12A2 6558
Affinity Capture-Western Homo sapiens
43 AIMP1 9255
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
44 SMAD7  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here