Gene description for F2R
Gene name coagulation factor II (thrombin) receptor
Gene symbol F2R
Other names/aliases CF2R
HTR
PAR-1
PAR1
TR
Species Homo sapiens
 Database cross references - F2R
ExoCarta ExoCarta_2149
Vesiclepedia VP_2149
Entrez Gene 2149
HGNC 3537
MIM 187930
UniProt P25116  
 F2R identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for F2R
Molecular Function
    G-protein alpha-subunit binding GO:0001965 ISS
    G-protein beta-subunit binding GO:0031681 ISS
    G-protein coupled receptor activity GO:0004930 TAS
    thrombin receptor activity GO:0015057 IDA
    protein binding GO:0005515 IPI
    receptor binding GO:0005102 IPI
Biological Process
    negative regulation of glomerular filtration GO:0003105 ISS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
    positive regulation of interleukin-6 secretion GO:2000778 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    regulation of sensory perception of pain GO:0051930 ISS
    negative regulation of renin secretion into blood stream GO:1900134 ISS
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of vasoconstriction GO:0045907 ISS
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 ISS
    regulation of blood coagulation GO:0030193 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IDA
    anatomical structure morphogenesis GO:0009653 TAS
    positive regulation of calcium ion transport GO:0051928 ISS
    positive regulation of smooth muscle contraction GO:0045987 ISS
    positive regulation of blood coagulation GO:0030194 IDA
    protein kinase C-activating G-protein coupled receptor signaling pathway GO:0007205 ISS
    positive regulation of cell migration GO:0030335 IMP
    STAT protein import into nucleus GO:0007262 IDA
    platelet dense granule organization GO:0060155 IC
    response to wounding GO:0009611 IDA
    release of sequestered calcium ion into cytosol GO:0051209 ISS
    G-protein coupled receptor signaling pathway GO:0007186 ISS
    establishment of synaptic specificity at neuromuscular junction GO:0007529 ISS
    positive regulation of cell proliferation GO:0008284 ISS
    tyrosine phosphorylation of STAT protein GO:0007260 IDA
    negative regulation of neuron apoptotic process GO:0043524 ISS
    activation of MAPKK activity GO:0000186 ISS
    positive regulation of interleukin-8 secretion GO:2000484 IDA
    response to lipopolysaccharide GO:0032496 ISS
    inflammatory response GO:0006954 ISS
    positive regulation of Rho protein signal transduction GO:0035025 ISS
    positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway GO:0051482 ISS
    positive regulation of collagen biosynthetic process GO:0032967 IDA
    thrombin receptor signaling pathway GO:0070493 IEA
    blood coagulation GO:0007596 TAS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    connective tissue replacement involved in inflammatory response wound healing GO:0002248 IDA
    regulation of interleukin-1 beta production GO:0032651 ISS
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IDA
    positive regulation of JAK-STAT cascade GO:0046427 IDA
    homeostasis of number of cells within a tissue GO:0048873 ISS
    phospholipase C-activating G-protein coupled receptor signaling pathway GO:0007200 IDA
    platelet activation GO:0030168 TAS
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISS
    negative regulation of cell proliferation GO:0008285 IDA
Subcellular Localization
    early endosome GO:0005769 IDA
    caveola GO:0005901 IDA
    extracellular region GO:0005576 TAS
    Golgi apparatus GO:0005794 TAS
    neuromuscular junction GO:0031594 ISS
    platelet dense tubular network GO:0031094 IDA
    late endosome GO:0005770 IDA
    cytosol GO:0005829 ISS
    postsynaptic membrane GO:0045211 ISS
    integral component of plasma membrane GO:0005887 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
 Experiment description of studies that identified F2R in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for F2R
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNX1 6642
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 GNA13 10672
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 ITGAV 3685
Invitro Homo sapiens
4 FABP6  
Affinity Capture-MS Homo sapiens
5 GNAI2 2771
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 GNA12 2768
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 GNA15 2769
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
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