Gene description for CHMP4A
Gene name charged multivesicular body protein 4A
Gene symbol CHMP4A
Other names/aliases C14orf123
CHMP4
CHMP4B
HSPC134
SHAX2
SNF7
SNF7-1
VPS32-1
VPS32A
Species Homo sapiens
 Database cross references - CHMP4A
ExoCarta ExoCarta_29082
Vesiclepedia VP_29082
Entrez Gene 29082
HGNC 20274
MIM 610051
UniProt Q9BY43  
 CHMP4A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CHMP4A
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    ATPase binding GO:0051117 IPI
Biological Process
    plasma membrane repair GO:0001778 IDA
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 IMP
    vesicle budding from membrane GO:0006900 IBA
    vesicle budding from membrane GO:0006900 IGI
    vesicle budding from membrane GO:0006900 IMP
    autophagy GO:0006914 IMP
    nucleus organization GO:0006997 IMP
    mitotic metaphase chromosome alignment GO:0007080 IMP
    membrane invagination GO:0010324 IMP
    protein transport GO:0015031 IEA
    macroautophagy GO:0016236 TAS
    nuclear membrane reassembly GO:0031468 IMP
    late endosome to vacuole transport via multivesicular body sorting pathway GO:0032511 IBA
    multivesicular body assembly GO:0036258 NAS
    multivesicular body assembly GO:0036258 TAS
    viral budding via host ESCRT complex GO:0039702 IDA
    viral budding via host ESCRT complex GO:0039702 IGI
    viral budding via host ESCRT complex GO:0039702 TAS
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 IDA
    viral budding from plasma membrane GO:0046761 IDA
    nervous system process GO:0050877 IMP
    protein polymerization GO:0051258 IMP
    vesicle fusion with vacuole GO:0051469 NAS
    multivesicular body-lysosome fusion GO:0061763 NAS
    midbody abscission GO:0061952 IMP
    multivesicular body sorting pathway GO:0071985 IDA
    multivesicular body sorting pathway GO:0071985 IMP
    membrane fission GO:0090148 NAS
    plasma membrane tubulation GO:0097320 IGI
    plasma membrane tubulation GO:0097320 IMP
    autophagosome maturation GO:0097352 IMP
    regulation of mitotic spindle assembly GO:1901673 IMP
    late endosome to lysosome transport GO:1902774 IMP
Subcellular Localization
    autophagosome membrane GO:0000421 IDA
    kinetochore GO:0000776 IDA
    ESCRT III complex GO:0000815 IBA
    ESCRT III complex GO:0000815 IDA
    ESCRT III complex GO:0000815 NAS
    ESCRT III complex GO:0000815 TAS
    nucleus GO:0005634 IDA
    nuclear pore GO:0005643 IDA
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 IDA
    endosome GO:0005768 IDA
    multivesicular body GO:0005771 IBA
    kinetochore microtubule GO:0005828 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    cytoplasmic side of plasma membrane GO:0009898 IBA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    membrane coat GO:0030117 IMP
    midbody GO:0030496 IDA
    multivesicular body membrane GO:0032585 IDA
    amphisome membrane GO:1904930 IDA
 Experiment description of studies that identified CHMP4A in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
12
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CHMP4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHMP2A 27243
Co-fractionation Homo sapiens
2 ABHD17A 81926
Affinity Capture-MS Homo sapiens
3 HSPE1 3336
Co-fractionation Homo sapiens
4 CHMP3 51652
Two-hybrid Homo sapiens
5 MYCN  
Affinity Capture-MS Homo sapiens
6 ZBTB2 57621
Affinity Capture-MS Homo sapiens
7 NUTF2 10204
Two-hybrid Homo sapiens
8 SMARCB1 6598
Co-fractionation Homo sapiens
9 ADSS 159
Co-fractionation Homo sapiens
10 SEP15 9403
Co-fractionation Homo sapiens
11 DCK 1633
Co-fractionation Homo sapiens
12 VPS4B 9525
Co-fractionation Homo sapiens
13 TAS2R41  
Affinity Capture-MS Homo sapiens
14 HOXC4  
Affinity Capture-MS Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 CDCA3 83461
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PDCD6IP 10015
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 NKD2 85409
Affinity Capture-MS Homo sapiens
19 TALDO1 6888
Co-fractionation Homo sapiens
20 CHMP4C 92421
Two-hybrid Homo sapiens
21 BTF3 689
Affinity Capture-MS Homo sapiens
22 RDH12 145226
Affinity Capture-MS Homo sapiens
23 GAK 2580
Affinity Capture-MS Homo sapiens
24 RBBP7 5931
Co-fractionation Homo sapiens
25 F2R 2149
Affinity Capture-Western Homo sapiens
26 COX15 1355
Affinity Capture-MS Homo sapiens
27 EFHD2 79180
Co-fractionation Homo sapiens
28 FSD1  
Affinity Capture-MS Homo sapiens
29 PPIA 5478
Affinity Capture-Western Homo sapiens
30 FCGR2A 2212
Affinity Capture-MS Homo sapiens
31 VTA1 51534
Affinity Capture-MS Homo sapiens
32 SOX2  
Affinity Capture-MS Homo sapiens
33 TFG 10342
Affinity Capture-MS Homo sapiens
34 Vps4b 20479
Affinity Capture-MS Mus musculus
35 PDZD8 118987
Affinity Capture-MS Homo sapiens
36 VAX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SYT17  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 CHRAC1  
Co-fractionation Homo sapiens
39 ARL6IP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HSPA2 3306
Affinity Capture-MS Homo sapiens
41 CDK13 8621
Two-hybrid Homo sapiens
42 TTC19  
Two-hybrid Homo sapiens
43 CHMP4B 128866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 WDYHV1  
Two-hybrid Homo sapiens
45 SLC25A32 81034
Affinity Capture-MS Homo sapiens
46 CHMP5 51510
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
47 RANBP1 5902
Co-fractionation Homo sapiens
48 EIF6 3692
Co-fractionation Homo sapiens
49 RWDD2B  
Affinity Capture-MS Homo sapiens
50 FOXR2  
Affinity Capture-MS Homo sapiens
51 FYTTD1  
Affinity Capture-MS Homo sapiens
52 Ptpn23 104831
Affinity Capture-MS Mus musculus
53 NFYA 4800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 OR2K2  
Affinity Capture-MS Homo sapiens
55 STAMBP 10617
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 IDH1 3417
Co-fractionation Homo sapiens
57 C11orf65  
Affinity Capture-MS Homo sapiens
58 Chmp4b 75608
Affinity Capture-MS Mus musculus
59 CPNE7 27132
Affinity Capture-MS Homo sapiens
60 DAXX  
Affinity Capture-MS Homo sapiens
61 RCN1 5954
Co-fractionation Homo sapiens
62 CC2D1A 54862
Two-hybrid Homo sapiens
63 VPS4A 27183
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
64 CHMP4A 29082
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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