Gene description for SLC25A32
Gene name solute carrier family 25 (mitochondrial folate carrier), member 32
Gene symbol SLC25A32
Other names/aliases MFT
MFTC
Species Homo sapiens
 Database cross references - SLC25A32
ExoCarta ExoCarta_81034
Vesiclepedia VP_81034
Entrez Gene 81034
HGNC 29683
MIM 610815
UniProt Q9H2D1  
 SLC25A32 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SLC25A32
Molecular Function
    folic acid transmembrane transporter activity GO:0008517 IBA
    folic acid transmembrane transporter activity GO:0008517 TAS
    FAD transmembrane transporter activity GO:0015230 IBA
    FAD transmembrane transporter activity GO:0015230 IMP
Biological Process
    folic acid metabolic process GO:0046655 TAS
    folate import into mitochondrion GO:1904947 IBA
    mitochondrial FAD transmembrane transport GO:1990548 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IGI
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified SLC25A32 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SLC25A32
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBFB 865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 DENR 8562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PDXP 57026
Affinity Capture-MS Homo sapiens
4 PLS1 5357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SERPINB3 6317
Affinity Capture-MS Homo sapiens
6 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
7 IMPDH1 3614
Affinity Capture-MS Homo sapiens
8 CRYAB 1410
Affinity Capture-MS Homo sapiens
9 SERPINB13 5275
Affinity Capture-MS Homo sapiens
10 CBR1 873
Affinity Capture-MS Homo sapiens
11 UBE2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
13 PEBP1 5037
Co-fractionation Homo sapiens
14 WIBG 84305
Affinity Capture-MS Homo sapiens
15 TCEA1 6917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SPAG7  
Affinity Capture-MS Homo sapiens
17 PMVK 10654
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 DBI 1622
Co-fractionation Homo sapiens
19 SERPINB2 5055
Affinity Capture-MS Homo sapiens
20 LASP1 3927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PNPO 55163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 HNRNPU 3192
Co-fractionation Homo sapiens
23 AKR1A1 10327
Affinity Capture-MS Homo sapiens
24 CTH 1491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 NEDD8-MDP1 100528064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PICALM 8301
Affinity Capture-MS Homo sapiens
27 CHMP4A 29082
Affinity Capture-MS Homo sapiens
28 IRGQ 126298
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PROSC 11212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CCDC25 55246
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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