Gene description for PICALM
Gene name phosphatidylinositol binding clathrin assembly protein
Gene symbol PICALM
Other names/aliases CALM
CLTH
LAP
Species Homo sapiens
 Database cross references - PICALM
ExoCarta ExoCarta_8301
Entrez Gene 8301
HGNC 15514
MIM 603025
UniProt Q13492  
 PICALM identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for PICALM
Molecular Function
    clathrin adaptor activity GO:0035615 IMP
    1-phosphatidylinositol binding GO:0005545 ISS
    clathrin heavy chain binding GO:0032050 IDA
    protein binding GO:0005515 IPI
    clathrin binding GO:0030276 ISS
Biological Process
    cell proliferation GO:0008283 IMP
    iron ion homeostasis GO:0055072 IMP
    positive regulation of neuron death GO:1901216 IMP
    positive regulation of beta-amyloid formation GO:1902004 IMP
    negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process GO:1902963 ISS
    endosomal transport GO:0016197 IMP
    synaptic vesicle maturation GO:0016188 ISS
    regulation of endocytosis GO:0030100 IMP
    positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process GO:1902961 ISS
    dendrite morphogenesis GO:0048813 IEA
    axonogenesis GO:0007409 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of gene expression GO:0010629 IMP
    receptor-mediated endocytosis GO:0006898 ISS
    regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process GO:1902959 IMP
    clathrin-mediated endocytosis GO:0072583 IMP
    hemopoiesis GO:0030097 IEA
    clathrin coat assembly GO:0048268 IMP
    negative regulation of receptor-mediated endocytosis GO:0048261 IDA
    cargo loading into vesicle GO:0035459 IMP
    iron ion import into cell GO:0097459 IMP
    vesicle-mediated transport GO:0016192 TAS
    receptor internalization GO:0031623 IMP
    regulation of protein localization GO:0032880 IDA
    protein complex assembly GO:0006461 TAS
Subcellular Localization
    AP-2 adaptor complex GO:0030122 IDA
    clathrin coat of coated pit GO:0030132 IDA
    neuronal cell body GO:0043025 IDA
    membrane GO:0016020 IDA
    perinuclear region of cytoplasm GO:0048471 ISS
    presynaptic membrane GO:0042734 ISS
    postsynaptic membrane GO:0045211 ISS
    nucleus GO:0005634 IDA
    neurofibrillary tangle GO:0097418 IMP
    coated pit GO:0005905 ISS
    Golgi apparatus GO:0005794 IEA
    vesicle GO:0031982 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified PICALM in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PICALM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLTCL1 8218
Invitro Homo sapiens
Invivo Homo sapiens
2 DDIT3 1649
Affinity Capture-Western Homo sapiens
3 PLCG1 5335
Invitro Homo sapiens
4 CASP3 836
Invitro Homo sapiens
5 CASP8  
Invitro Homo sapiens
6 AP2A1 160
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which PICALM is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis TAS Reactome





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