Gene description for DOK4
Gene name docking protein 4
Gene symbol DOK4
Other names/aliases IRS-5
IRS5
Species Homo sapiens
 Database cross references - DOK4
ExoCarta ExoCarta_55715
Vesiclepedia VP_55715
Entrez Gene 55715
HGNC 19868
MIM 608333
UniProt Q8TEW6  
 DOK4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DOK4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    nervous system development GO:0007399 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DOK4 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DOK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
2 KLHL20  
Affinity Capture-MS Homo sapiens
3 MIER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 STK24 8428
Affinity Capture-MS Homo sapiens
5 RHOU 58480
Reconstituted Complex Homo sapiens
6 ERBB2 2064
Protein-peptide Homo sapiens
7 RCOR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SMARCAD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 AHCYL1 10768
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CCDC43 124808
Affinity Capture-MS Homo sapiens
11 QRSL1  
Affinity Capture-MS Homo sapiens
12 ELK4 2005
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
13 OPRK1  
Affinity Capture-Western Homo sapiens
14 INCA1  
Two-hybrid Homo sapiens
15 PBK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SRP19 6728
Affinity Capture-MS Homo sapiens
17 UBE3A 7337
Affinity Capture-MS Homo sapiens
18 OPRD1  
Affinity Capture-Western Homo sapiens
19 THRAP3 9967
Cross-Linking-MS (XL-MS) Homo sapiens
20 ANAPC16  
Affinity Capture-MS Homo sapiens
21 EGFR 1956
Protein-peptide Homo sapiens
22 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
23 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RET 5979
Affinity Capture-Western Homo sapiens
25 CTBP1 1487
Affinity Capture-MS Homo sapiens
26 SUPT4H1  
Affinity Capture-MS Homo sapiens
27 PRAMEF10  
Affinity Capture-MS Homo sapiens
28 SUPT5H 6829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 BTF3L4 91408
Affinity Capture-MS Homo sapiens
31 ECE1 1889
Cross-Linking-MS (XL-MS) Homo sapiens
32 PUS7 54517
Affinity Capture-MS Homo sapiens
33 KIAA1598 57698
Affinity Capture-MS Homo sapiens
34 POLR3G  
Affinity Capture-MS Homo sapiens
35 PHKA2 5256
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 AHCYL2 23382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KBTBD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 KDM1A 23028
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KIF1BP 26128
Affinity Capture-MS Homo sapiens
40 CMSS1  
Affinity Capture-MS Homo sapiens
41 UBLCP1  
Affinity Capture-MS Homo sapiens
42 BCL7B  
Affinity Capture-MS Homo sapiens
43 GSE1 23199
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PHKB 5257
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
46 PAX8  
Two-hybrid Homo sapiens
47 OPRM1 4988
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 ANK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CWC22 57703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 WBP4  
Affinity Capture-MS Homo sapiens
51 HMG20A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PICALM 8301
Affinity Capture-MS Homo sapiens
53 FHL2 2274
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RCOR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PFDN5 5204
Two-hybrid Homo sapiens
56 PARN  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DOK4 is involved
PathwayEvidenceSource
Axon guidance TAS Reactome
Developmental Biology TAS Reactome
Nervous system development TAS Reactome
RET signaling TAS Reactome





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