Experiment description of studies that identified KIF1BP in exosomes

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for KIF1BP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 KIF3B 9371 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 KIF16B 55614 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 ACTR2 10097 Co-fractionation Homo sapiens Proximity Label-MS Homo sapiens 4 GTF2F1 2962 Co-fractionation Homo sapiens 5 CHIC1   Affinity Capture-MS Homo sapiens 6 STK25 10494 Affinity Capture-MS Homo sapiens 7 COIL   Proximity Label-MS Homo sapiens 8 C2orf48   Affinity Capture-MS Homo sapiens 9 SNX33 257364 Affinity Capture-MS Homo sapiens 10 SH3GL1 6455 Co-fractionation Homo sapiens 11 KIF13A 63971 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 TPTE2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 PUS7 54517 Affinity Capture-MS Homo sapiens 14 EFTUD1 79631 Co-fractionation Homo sapiens 15 DPEP1 1800 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 16 PNKD   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 CENPA   Proximity Label-MS Homo sapiens 18 PPP1CC 5501 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 KIF13B 23303 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 TOP2A 7153 Affinity Capture-MS Homo sapiens 21 NDE1 54820 Affinity Capture-MS Homo sapiens 22 BCL7B   Affinity Capture-MS Homo sapiens 23 ACTR3 10096 Co-fractionation Homo sapiens 24 RFXANK   Affinity Capture-MS Homo sapiens 25 ZNF638 27332 Two-hybrid Homo sapiens 26 ANP32B 10541 Co-fractionation Homo sapiens 27 ACTR1A 10121 Proximity Label-MS Homo sapiens 28 DNAJB1 3337 Affinity Capture-MS Homo sapiens 29 PLD2 5338 Affinity Capture-MS Homo sapiens 30 ELP4   Affinity Capture-MS Homo sapiens 31 KRT83   Affinity Capture-MS Homo sapiens 32 NPAS1   Affinity Capture-MS Homo sapiens 33 PLCB3 5331 Affinity Capture-MS Homo sapiens 34 KIF3A 11127 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 NIF3L1 60491 Co-fractionation Homo sapiens 36 STX12 23673 Affinity Capture-MS Homo sapiens 37 KIF14 9928 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 FAS 355 Proximity Label-MS Homo sapiens 39 ATXN2 6311 Two-hybrid Homo sapiens 40 KRT8 3856 Proximity Label-MS Homo sapiens 41 PPP1CA 5499 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 NSUN2 54888 Co-fractionation Homo sapiens 43 STARD9 57519 Affinity Capture-MS Homo sapiens 44 RTFDC1   Affinity Capture-MS Homo sapiens 45 PSMD4 5710 Co-fractionation Homo sapiens 46 MAP1B 4131 Co-fractionation Homo sapiens 47 MAPRE1 22919 Affinity Capture-MS Homo sapiens 48 Kif18a   Affinity Capture-MS Mus musculus 49 PANK4 55229 Co-fractionation Homo sapiens 50 KPNA4 3840 Co-fractionation Homo sapiens 51 SNRNP200 23020 Co-fractionation Homo sapiens 52 PTP4A3   Affinity Capture-MS Homo sapiens 53 BBS1 582 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 54 VCP 7415 Affinity Capture-MS Homo sapiens 55 NTRK1 4914 Affinity Capture-MS Homo sapiens 56 GORASP2 26003 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 ALDH3B1 221 Affinity Capture-MS Homo sapiens 58 DHFRL1   Proximity Label-MS Homo sapiens 59 TPRKB 51002 Co-fractionation Homo sapiens 60 CEP76   Two-hybrid Homo sapiens 61 H2AFY 9555 Co-fractionation Homo sapiens 62 Ksr1   Affinity Capture-MS Mus musculus 63 PRKACB 5567 Co-fractionation Homo sapiens 64 METTL1 4234 Co-fractionation Homo sapiens 65 UBXN6 80700 Affinity Capture-MS Homo sapiens 66 KIF18A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 67 CD3EAP   Proximity Label-MS Homo sapiens 68 DOK2 9046 Two-hybrid Homo sapiens 69 KIF1B 23095 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 EPB41L1 2036 Affinity Capture-MS Homo sapiens 71 ZBTB2 57621 Affinity Capture-MS Homo sapiens 72 HADHA 3030 Co-fractionation Homo sapiens 73 KIF15 56992 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 74 AHCYL1 10768 Co-fractionation Homo sapiens 75 TIGD4 201798 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 76 SLK 9748 Affinity Capture-MS Homo sapiens 77 OSGEP 55644 Co-fractionation Homo sapiens 78 FAM90A1   Affinity Capture-MS Homo sapiens 79 METTL21B   Affinity Capture-MS Homo sapiens 80 WIBG 84305 Affinity Capture-MS Homo sapiens 81 MMP13   Affinity Capture-MS Homo sapiens 82 BAIAP2L1 55971 Affinity Capture-MS Homo sapiens 83 SNX9 51429 Affinity Capture-MS Homo sapiens 84 DHRS13   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 85 RPA3 6119 Proximity Label-MS Homo sapiens 86 VPS37D 155382 Affinity Capture-MS Homo sapiens 87 SAV1   Proximity Label-MS Homo sapiens 88 Kif15   Affinity Capture-MS Mus musculus 89 GSTK1 373156 Affinity Capture-MS Homo sapiens 90 TP53RK 112858 Co-fractionation Homo sapiens 91 MFN2 9927 Proximity Label-MS Homo sapiens 92 PARP1 142 Proximity Label-MS Homo sapiens 93 DNAJB13   Affinity Capture-MS Homo sapiens 94 FOXQ1   Affinity Capture-MS Homo sapiens 95 KIF3C 3797 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 96 FSCN1 6624 Affinity Capture-MS Homo sapiens 97 ARAF 369 Affinity Capture-MS Homo sapiens 98 DOK4 55715 Affinity Capture-MS Homo sapiens 99 U2SURP 23350 Co-fractionation Homo sapiens 100 PLEKHF1 79156 Two-hybrid Homo sapiens 101 MAP2K2 5605 Affinity Capture-MS Homo sapiens 102 KIF1A 547 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 103 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 104 ZNF670   Two-hybrid Homo sapiens 105 WDR4 10785 Co-fractionation Homo sapiens 106 FCGRT 2217 Affinity Capture-MS Homo sapiens 107 CALU 813 Co-fractionation Homo sapiens 108 SAP18 10284 Affinity Capture-MS Homo sapiens 109 CCDC89   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 110 LMAN1 3998 Proximity Label-MS Homo sapiens 111 SEPT10 151011 Proximity Label-MS Homo sapiens 112 HBP1   Two-hybrid Homo sapiens 113 COX8A   Proximity Label-MS Homo sapiens 114 GNL1 2794 Co-fractionation Homo sapiens 115 FKBP5 2289 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 116 TERF1 7013 Two-hybrid Homo sapiens 117 KDM1A 23028 Proximity Label-MS Homo sapiens 118 EIF3J 8669 Affinity Capture-MS Homo sapiens 119 KIF18B 146909 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 120 EEF1D 1936 Co-fractionation Homo sapiens 121 SH3GLB1 51100 Co-fractionation Homo sapiens 122 SERBP1 26135 Affinity Capture-MS Homo sapiens 123 PTGES3 10728 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 124 SNW1 22938 Affinity Capture-MS Homo sapiens 125 QRSL1   Affinity Capture-MS Homo sapiens 126 RGR   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 127 KIF1C 10749 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 128 ANKRD54   Affinity Capture-MS Homo sapiens 129 GPKOW   Affinity Capture-MS Homo sapiens 130 RANGAP1 5905 Co-fractionation Homo sapiens 131 UFM1 51569 Co-fractionation Homo sapiens View the network

Pathways in which KIF1BP is involved

No pathways found