Gene description for KIF3B
Gene name kinesin family member 3B
Gene symbol KIF3B
Other names/aliases FLA8
HH0048
KLP-11
Species Homo sapiens
 Database cross references - KIF3B
ExoCarta ExoCarta_9371
Vesiclepedia VP_9371
Entrez Gene 9371
HGNC 6320
MIM 603754
UniProt O15066  
 KIF3B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for KIF3B
Molecular Function
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 TAS
    ATP hydrolysis activity GO:0016887 IBA
    small GTPase binding GO:0031267 IPI
    intraciliary transport particle B binding GO:0120170 IEA
Biological Process
    microtubule-based movement GO:0007018 IBA
    mitotic spindle organization GO:0007052 TAS
    mitotic centrosome separation GO:0007100 TAS
    determination of left/right symmetry GO:0007368 TAS
    anterograde axonal transport GO:0008089 TAS
    vesicle-mediated transport GO:0016192 IEA
    positive regulation of cytokinesis GO:0032467 IEA
    opsin transport GO:0036372 IMP
    intraciliary transport GO:0042073 IMP
    cilium assembly GO:0060271 IMP
    plus-end-directed vesicle transport along microtubule GO:0072383 TAS
    mitotic spindle assembly GO:0090307 IMP
    anterograde dendritic transport of neurotransmitter receptor complex GO:0098971 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 NAS
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    plus-end kinesin complex GO:0005873 TAS
    microtubule GO:0005874 IBA
    spindle microtubule GO:0005876 NAS
    cilium GO:0005929 ISS
    cilium GO:0005929 TAS
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 HDA
    kinesin II complex GO:0016939 IDA
    dendrite GO:0030425 ISS
    midbody GO:0030496 IEA
    dendrite cytoplasm GO:0032839 IEA
    dendritic spine GO:0043197 IEA
    extracellular exosome GO:0070062 HDA
    ciliary tip GO:0097542 TAS
    glutamatergic synapse GO:0098978 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF3B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for KIF3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WIZ 58525
Co-fractionation Homo sapiens
2 STK3 6788
Affinity Capture-MS Homo sapiens
3 RAD21 5885
Affinity Capture-Western Homo sapiens
4 Hax1  
Affinity Capture-MS Mus musculus
5 KIF3A 11127
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
6 LMO4  
Two-hybrid Homo sapiens
7 RAB5A 5868
Affinity Capture-Western Homo sapiens
8 AKAP12 9590
Affinity Capture-MS Homo sapiens
9 PFN1 5216
Cross-Linking-MS (XL-MS) Homo sapiens
10 POLR3H  
Co-fractionation Homo sapiens
11 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
12 RPAP2  
Co-fractionation Homo sapiens
13 VHL  
Affinity Capture-Western Homo sapiens
14 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
15 PARK2  
Affinity Capture-MS Homo sapiens
16 Mphosph9  
Affinity Capture-MS Mus musculus
17 KIFAP3 22920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 NCKAP5  
Affinity Capture-MS Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
20 NXF1 10482
Affinity Capture-RNA Homo sapiens
21 RAB4A 5867
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 PRKCI 5584
Co-fractionation Homo sapiens
23 LMO2  
Two-hybrid Homo sapiens
24 MOV10 4343
Affinity Capture-RNA Homo sapiens
25 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 IFT172 26160
Co-fractionation Homo sapiens
27 NTRK1 4914
Affinity Capture-MS Homo sapiens
28 SAV1  
Affinity Capture-MS Homo sapiens
29 GOLGA3 2802
Co-fractionation Homo sapiens
30 MAP3K11 4296
Two-hybrid Homo sapiens
31 PSMC5 5705
Affinity Capture-MS Homo sapiens
32 PRC1 9055
Two-hybrid Homo sapiens
33 DYRK2 8445
Affinity Capture-MS Homo sapiens
34 Sptlc2  
Affinity Capture-MS Mus musculus
35 HNRNPU 3192
Cross-Linking-MS (XL-MS) Homo sapiens
36 STK4 6789
Affinity Capture-MS Homo sapiens
37 HSPD1 3329
Cross-Linking-MS (XL-MS) Homo sapiens
38 KIF3C 3797
Affinity Capture-MS Homo sapiens
39 NEDD4L 23327
Two-hybrid Homo sapiens
40 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 Homer1  
Affinity Capture-MS Mus musculus
42 Magoh  
Affinity Capture-MS Mus musculus
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