Gene description for PRKCI
Gene name protein kinase C, iota
Gene symbol PRKCI
Other names/aliases DXS1179E
PKCI
nPKC-iota
Species Homo sapiens
 Database cross references - PRKCI
ExoCarta ExoCarta_5584
Entrez Gene 5584
HGNC 9404
MIM 600539
UniProt P41743  
 PRKCI identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for PRKCI
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase C activity GO:0004697 ISS
    protein domain specific binding GO:0019904 IEA
    metal ion binding GO:0046872 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
    ATP binding GO:0005524 TAS
    phospholipid binding GO:0005543 IDA
    protein kinase activity GO:0004672 IDA
Biological Process
    establishment of apical/basal cell polarity GO:0035089 IEA
    protein targeting to membrane GO:0006612 NAS
    intracellular signal transduction GO:0035556 IEA
    response to interleukin-1 GO:0070555 IEA
    cytoskeleton organization GO:0007010 NAS
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 TAS
    positive regulation of endothelial cell apoptotic process GO:2000353 IMP
    actin filament organization GO:0007015 IEA
    positive regulation of neuron projection development GO:0010976 IMP
    negative regulation of apoptotic process GO:0043066 TAS
    bicellular tight junction assembly GO:0070830 TAS
    Golgi vesicle budding GO:0048194 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    cell-cell junction organization GO:0045216 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    protein phosphorylation GO:0006468 IDA
    eye photoreceptor cell development GO:0042462 IEA
    secretion GO:0046903 NAS
    membrane organization GO:0061024 NAS
    negative regulation of glial cell apoptotic process GO:0034351 IMP
    vesicle-mediated transport GO:0016192 TAS
    cellular response to insulin stimulus GO:0032869 ISS
    cell junction assembly GO:0034329 TAS
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISS
    positive regulation of glial cell proliferation GO:0060252 IMP
    cell migration GO:0016477 IEA
    positive regulation of glucose import GO:0046326 ISS
    negative regulation of neuron apoptotic process GO:0043524 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi membrane GO:0000139 IEA
    bicellular tight junction GO:0005923 IEA
    extracellular exosome GO:0070062 IDA
    cell leading edge GO:0031252 IEA
    intercellular bridge GO:0045171 IDA
    nucleus GO:0005634 IDA
    protein complex GO:0043234 IEA
    microtubule cytoskeleton GO:0015630 IDA
    apical plasma membrane GO:0016324 IEA
    endosome GO:0005768 IEA
    Schmidt-Lanterman incisure GO:0043220 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified PRKCI in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKCI
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SQSTM1 8878
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 RAB4A 5867
Affinity Capture-Western Homo sapiens
3 CENTA1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
4 FRS2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 YWHAZ 7534
Reconstituted Complex Homo sapiens
6 GAPDH 2597
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 PARD6A  
Two-hybrid Homo sapiens
8 MAP2K5  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
9 BAD  
Reconstituted Complex Homo sapiens
10 PARD6G  
Invitro Homo sapiens
Two-hybrid Homo sapiens
11 PDPK1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 SMG1 23049
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which PRKCI is involved
PathwayEvidenceSource
p75NTR recruits signalling complexes TAS Reactome
Tight junction interactions TAS Reactome





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