Gene description for SMG1
Gene name SMG1 phosphatidylinositol 3-kinase-related kinase
Gene symbol SMG1
Other names/aliases 61E3.4
ATX
LIP
Species Homo sapiens
 Database cross references - SMG1
ExoCarta ExoCarta_23049
Vesiclepedia VP_23049
Entrez Gene 23049
HGNC 30045
MIM 607032
UniProt Q96Q15  
 SMG1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMG1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 NAS
    poly(A) RNA binding GO:0044822 IDA
    protein dimerization activity GO:0046983 IBA
    ATP binding GO:0005524 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    gene expression GO:0010467 TAS
    mRNA export from nucleus GO:0006406 TAS
    phosphatidylinositol phosphorylation GO:0046854 IDA
    peptidyl-serine phosphorylation GO:0018105 TAS
    response to stress GO:0006950 IDA
    double-strand break repair via homologous recombination GO:0000724 IBA
    protein autophosphorylation GO:0046777 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SMG1 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UPF3A  
Invivo Homo sapiens
2 UPF1 5976
Invivo Homo sapiens
3 CDH5  
Invivo Homo sapiens
4 UPF2 26019
Invivo Homo sapiens
5 PRKCI 5584
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SMG1 is involved
PathwayEvidenceSource
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome





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