Gene description for UPF2
Gene name UPF2 regulator of nonsense transcripts homolog (yeast)
Gene symbol UPF2
Other names/aliases HUPF2
RENT2
smg-3
Species Homo sapiens
 Database cross references - UPF2
ExoCarta ExoCarta_26019
Vesiclepedia VP_26019
Entrez Gene 26019
HGNC 17854
MIM 605529
UniProt Q9HAU5  
 UPF2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UPF2
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    telomeric DNA binding GO:0042162 IDA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IBA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IMP
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    liver development GO:0001889 IEA
    mRNA export from nucleus GO:0006406 TAS
    animal organ regeneration GO:0031100 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    exon-exon junction complex GO:0035145 IBA
    exon-exon junction complex GO:0035145 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified UPF2 in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UPF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Two-hybrid Homo sapiens
2 ACTR2 10097
Affinity Capture-MS Homo sapiens
3 HBB 3043
Affinity Capture-RNA Homo sapiens
4 LGR4 55366
Affinity Capture-MS Homo sapiens
5 TK1 7083
Two-hybrid Homo sapiens
6 DCP1B  
Two-hybrid Homo sapiens
7 WNK1 65125
Two-hybrid Homo sapiens
8 LDHA 3939
Two-hybrid Homo sapiens
9 EIF4A1 1973
Two-hybrid Homo sapiens
10 RSRC2  
Two-hybrid Homo sapiens
11 RAB2B 84932
Affinity Capture-MS Homo sapiens
12 PRPF40A 55660
Co-fractionation Homo sapiens
13 SMARCD2 6603
Co-fractionation Homo sapiens
14 MAP1LC3B 81631
Two-hybrid Homo sapiens
15 ZNF44  
Two-hybrid Homo sapiens
16 CENPU  
Two-hybrid Homo sapiens
17 SNRNP70 6625
Co-fractionation Homo sapiens
18 RNPS1 10921
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
19 EXOSC2 23404
Affinity Capture-Western Homo sapiens
20 ZNF408  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 NDUFA7 4701
Two-hybrid Homo sapiens
22 EIF4A3 9775
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
23 ZGPAT  
Two-hybrid Homo sapiens
24 HMGA1 3159
Two-hybrid Homo sapiens
25 GSPT1 2935
Affinity Capture-MS Homo sapiens
26 EXOSC4 54512
Affinity Capture-Western Homo sapiens
27 EXOSC8  
Two-hybrid Homo sapiens
28 LSM1 27257
Two-hybrid Homo sapiens
29 E4F1  
Two-hybrid Homo sapiens
30 SERF2 10169
Two-hybrid Homo sapiens
31 EXOSC6 118460
Two-hybrid Homo sapiens
32 CALR 811
Two-hybrid Homo sapiens
33 MECP2 4204
Affinity Capture-MS Homo sapiens
34 MYCN  
Affinity Capture-MS Homo sapiens
35 RRP7A 27341
Two-hybrid Homo sapiens
36 MTERF3  
Two-hybrid Homo sapiens
37 RBM8A 9939
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 H2AFZ 3015
Affinity Capture-MS Homo sapiens
39 POLD1 5424
Affinity Capture-Western Homo sapiens
40 NOMO1 23420
Two-hybrid Homo sapiens
41 TOP3B 8940
Two-hybrid Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
44 XRN1 54464
Affinity Capture-Western Homo sapiens
45 BLM 641
Two-hybrid Homo sapiens
46 COMTD1 118881
Two-hybrid Homo sapiens
47 ZBTB48  
Two-hybrid Homo sapiens
48 ABCF2 10061
Two-hybrid Homo sapiens
49 RPL5 6125
Two-hybrid Homo sapiens
50 AKR7L 246181
Affinity Capture-MS Homo sapiens
51 CYBA 1535
Two-hybrid Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 TRIP10 9322
Co-fractionation Homo sapiens
54 HS3ST3A1 9955
Two-hybrid Homo sapiens
55 ENO1 2023
Two-hybrid Homo sapiens
56 HECTD1 25831
Affinity Capture-MS Homo sapiens
57 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
58 PRPF31 26121
Co-fractionation Homo sapiens
59 GLTSCR2  
Two-hybrid Homo sapiens
60 RMDN3 55177
Proximity Label-MS Homo sapiens
61 DPF2  
Two-hybrid Homo sapiens
62 MRPL40 64976
Two-hybrid Homo sapiens
63 KIF15 56992
Affinity Capture-MS Homo sapiens
64 AGPAT1 10554
Two-hybrid Homo sapiens
65 KRT18 3875
Two-hybrid Homo sapiens
66 XPO1 7514
Affinity Capture-MS Homo sapiens
67 SMARCD1 6602
Co-fractionation Homo sapiens
68 TYMS 7298
Two-hybrid Homo sapiens
69 SREBF1 6720
Negative Genetic Homo sapiens
70 RPS6 6194
Two-hybrid Homo sapiens
71 EGFR 1956
Negative Genetic Homo sapiens
72 RPL8 6132
Two-hybrid Homo sapiens
73 GADD45GIP1  
Two-hybrid Homo sapiens
74 FUS 2521
Dosage Rescue Homo sapiens
75 UPF3A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 GIGYF2 26058
Cross-Linking-MS (XL-MS) Homo sapiens
77 NOB1  
Two-hybrid Homo sapiens
78 MYC  
Affinity Capture-MS Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 ATP1B3 483
Two-hybrid Homo sapiens
81 TP63  
Affinity Capture-MS Homo sapiens
82 MMS22L  
Co-fractionation Homo sapiens
83 SKIV2L2 23517
Two-hybrid Homo sapiens
84 UPF3B 65109
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
85 MYB  
Reconstituted Complex Homo sapiens
86 UPF2 26019
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
87 SUMO3 6612
Two-hybrid Homo sapiens
88 PAIP1 10605
Co-fractionation Homo sapiens
89 TUBB2A 7280
Two-hybrid Homo sapiens
90 RPL13 6137
Two-hybrid Homo sapiens
91 EXOSC10 5394
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
92 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
93 PHB2 11331
Two-hybrid Homo sapiens
94 RPS25 6230
Two-hybrid Homo sapiens
95 ZFP36  
Two-hybrid Homo sapiens
96 RBM25 58517
Co-fractionation Homo sapiens
97 MAP1A 4130
Two-hybrid Homo sapiens
98 LGALS3 3958
Co-fractionation Homo sapiens
99 MEN1 4221
Affinity Capture-MS Homo sapiens
100 TNNI2 7136
Two-hybrid Homo sapiens
101 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
102 PITX1  
Two-hybrid Homo sapiens
103 RPS15 6209
Two-hybrid Homo sapiens
104 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
105 PUF60 22827
Two-hybrid Homo sapiens
106 EPB41L2 2037
Co-fractionation Homo sapiens
107 ESR1  
Affinity Capture-MS Homo sapiens
108 UFL1 23376
Affinity Capture-MS Homo sapiens
109 MIF 4282
Two-hybrid Homo sapiens
110 ART4  
Two-hybrid Homo sapiens
111 UPF1 5976
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
112 TRIM28 10155
Affinity Capture-MS Homo sapiens
113 RPS7 6201
Two-hybrid Homo sapiens
114 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
115 SMG1 23049
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
116 SERBP1 26135
Two-hybrid Homo sapiens
117 KRAS 3845
Negative Genetic Homo sapiens
118 CIZ1  
Two-hybrid Homo sapiens
119 HBZ  
Two-hybrid Homo sapiens
120 SMARCD3  
Co-fractionation Homo sapiens
121 HSF2BP  
Two-hybrid Homo sapiens
122 SAP18 10284
Co-fractionation Homo sapiens
123 DCP2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
124 EXOSC1 51013
Two-hybrid Homo sapiens
125 PARN  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
126 CASC3  
Co-fractionation Homo sapiens
127 XRN2 22803
Two-hybrid Homo sapiens
View the network image/svg+xml



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