Gene description for UPF2
Gene name UPF2 regulator of nonsense transcripts homolog (yeast)
Gene symbol UPF2
Other names/aliases HUPF2
RENT2
smg-3
Species Homo sapiens
 Database cross references - UPF2
ExoCarta ExoCarta_26019
Vesiclepedia VP_26019
Entrez Gene 26019
HGNC 17854
MIM 605529
UniProt Q9HAU5  
 UPF2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UPF2
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
Biological Process
    organ regeneration GO:0031100 IEA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    gene expression GO:0010467 TAS
    liver development GO:0001889 IEA
    mRNA export from nucleus GO:0006406 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    exon-exon junction complex GO:0035145 IDA
 Experiment description of studies that identified UPF2 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UPF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UPF3B 65109
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 PARN  
Affinity Capture-Western Homo sapiens
3 MAP1A 4130
Two-hybrid Homo sapiens
4 XRN2 22803
Two-hybrid Homo sapiens
5 XRN1 54464
Affinity Capture-Western Homo sapiens
6 EIF4A1 1973
Two-hybrid Homo sapiens
7 RPS25 6230
Two-hybrid Homo sapiens
8 RPS15 6209
Two-hybrid Homo sapiens
9 EXOSC2 23404
Affinity Capture-Western Homo sapiens
10 DCP2  
Affinity Capture-Western Homo sapiens
11 UPF2 26019
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 LSM1 27257
Two-hybrid Homo sapiens
13 SRRM1 10250
Invivo Homo sapiens
14 EXOSC10 5394
Affinity Capture-Western Homo sapiens
15 UPF1 5976
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 EXOSC4 54512
Affinity Capture-Western Homo sapiens
17 UPF3A  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
18 DCP1A 55802
Invivo Homo sapiens
19 SMG1 23049
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which UPF2 is involved
PathwayEvidenceSource
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome





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