Gene description for UPF3B
Gene name UPF3 regulator of nonsense transcripts homolog B (yeast)
Gene symbol UPF3B
Other names/aliases HUPF3B
MRX62
MRXS14
RENT3B
UPF3X
Species Homo sapiens
 Database cross references - UPF3B
ExoCarta ExoCarta_65109
Vesiclepedia VP_65109
Entrez Gene 65109
HGNC 20439
MIM 300298
UniProt Q9BZI7  
 UPF3B identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UPF3B
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    mRNA binding GO:0003729 IDA
    nucleocytoplasmic transporter activity GO:0005487 NAS
Biological Process
    mRNA 3'-end processing GO:0031124 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    positive regulation of translation GO:0045727 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    gene expression GO:0010467 TAS
    mRNA export from nucleus GO:0006406 TAS
    RNA splicing GO:0008380 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 NAS
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    exon-exon junction complex GO:0035145 IDA
 Experiment description of studies that identified UPF3B in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UPF3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCRS1  
Two-hybrid Homo sapiens
2 TTC19  
Two-hybrid Homo sapiens
3 UPF2 26019
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 SRRM1 10250
Invivo Homo sapiens
5 UPF1 5976
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 UPF3A  
Affinity Capture-Western Homo sapiens
7 EIF6 3692
Two-hybrid Homo sapiens
View the network image/svg+xml



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