Gene description for EIF6
Gene name eukaryotic translation initiation factor 6
Gene symbol EIF6
Other names/aliases CAB
EIF3A
ITGB4BP
b(2)gcn
eIF-6
p27(BBP)
p27BBP
Species Homo sapiens
 Database cross references - EIF6
ExoCarta ExoCarta_3692
Entrez Gene 3692
HGNC 6159
MIM 602912
UniProt P56537  
 EIF6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for EIF6
Molecular Function
    translation initiation factor activity GO:0003743 IEA
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IDA
    ribosomal large subunit binding GO:0043023 IEA
Biological Process
    ribosomal subunit export from nucleus GO:0000054 IEA
    mature ribosome assembly GO:0042256 IMP
    translational initiation GO:0006413 IEA
    ribosomal large subunit biogenesis GO:0042273 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IEA
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 IDA
    lamin filament GO:0005638 IEA
 Experiment description of studies that identified EIF6 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for EIF6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Two-hybrid Homo sapiens
2 FHL2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 NPEPPS 9520
Affinity Capture-MS Homo sapiens
4 Sep-03  
Two-hybrid Homo sapiens
5 WFS1  
Two-hybrid Homo sapiens
6 HAS1 3036
Affinity Capture-MS Homo sapiens
7 FLJ10094  
Two-hybrid Homo sapiens
8 RPL6 6128
Two-hybrid Homo sapiens
9 SORBS1 10580
Affinity Capture-MS Homo sapiens
10 CRELD1  
Two-hybrid Homo sapiens
11 EIF3C 8663
Co-purification Homo sapiens
12 RIF1  
Two-hybrid Homo sapiens
13 FLJ20534  
Two-hybrid Homo sapiens
14 ALDH2 217
Two-hybrid Homo sapiens
15 ABCF1 23
Two-hybrid Homo sapiens
16 PAICS 10606
Affinity Capture-MS Homo sapiens
17 CGI-115  
Affinity Capture-MS Homo sapiens
18 FUNDC2  
Two-hybrid Homo sapiens
19 YARS 8565
Affinity Capture-MS Homo sapiens
20 RPL3 6122
Affinity Capture-MS Homo sapiens
21 XRN2 22803
Affinity Capture-MS Homo sapiens
22 ARF6 382
Affinity Capture-MS Homo sapiens
23 FUS 2521
Affinity Capture-MS Homo sapiens
24 PSME1 5720
Two-hybrid Homo sapiens
25 RPL34 6164
Affinity Capture-MS Homo sapiens
26 OAT 4942
Affinity Capture-MS Homo sapiens
27 ACAP3 116983
Two-hybrid Homo sapiens
28 POLA2  
Two-hybrid Homo sapiens
29 OFD1 8481
Two-hybrid Homo sapiens
30 FIBP  
Affinity Capture-MS Homo sapiens
31 AKT1S1 84335
Two-hybrid Homo sapiens
32 LOC387753  
Affinity Capture-MS Homo sapiens
33 MRPS31  
Two-hybrid Homo sapiens
34 KIAA1377  
Two-hybrid Homo sapiens
35 ITGB4 3691
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
36 BCCIP 56647
Affinity Capture-MS Homo sapiens
37 EIF3B 8662
Co-purification Homo sapiens
38 EIF3J 8669
Co-purification Homo sapiens
39 HIP1 3092
Two-hybrid Homo sapiens
40 GIT1 28964
Two-hybrid Homo sapiens
41 GNB2L1 10399
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 RGS10 6001
Affinity Capture-MS Homo sapiens
43 PRKCB 5579
Affinity Capture-MS Homo sapiens
44 UPF3B  
Two-hybrid Homo sapiens
45 ACTG1 71
Two-hybrid Homo sapiens
46 GTPBP4 23560
Affinity Capture-MS Homo sapiens
47 USP33  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which EIF6 is involved
No pathways found





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