Gene description for EIF2AK2
Gene name eukaryotic translation initiation factor 2-alpha kinase 2
Gene symbol EIF2AK2
Other names/aliases EIF2AK1
PKR
PPP1R83
PRKR
Species Homo sapiens
 Database cross references - EIF2AK2
ExoCarta ExoCarta_5610
Entrez Gene 5610
HGNC 9437
MIM 176871
UniProt P19525  
 EIF2AK2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Thymus 23844026    
 Gene ontology annotations for EIF2AK2
Molecular Function
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 TAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    double-stranded RNA binding GO:0003725 IDA
    protein kinase activity GO:0004672 IMP
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    ATP binding GO:0005524 IEA
    poly(A) RNA binding GO:0044822 IDA
    protein phosphatase type 2A regulator activity GO:0008601 TAS
Biological Process
    protein autophosphorylation GO:0046777 IMP
    innate immune response GO:0045087 IEA
    evasion or tolerance by virus of host immune response GO:0030683 TAS
    positive regulation of stress-activated MAPK cascade GO:0032874 ISS
    negative regulation of osteoblast proliferation GO:0033689 IMP
    negative regulation of apoptotic process GO:0043066 IEA
    modulation by virus of host morphology or physiology GO:0019048 TAS
    positive regulation of chemokine production GO:0032722 ISS
    negative regulation of viral genome replication GO:0045071 IMP
    defense response to virus GO:0051607 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    response to interferon-alpha GO:0035455 IDA
    regulation of hematopoietic progenitor cell differentiation GO:1901532 ISS
    translation GO:0006412 IEA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of cytokine production GO:0001819 ISS
    negative regulation of translation GO:0017148 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    positive regulation of NIK/NF-kappaB signaling GO:1901224 ISS
    negative regulation of cell proliferation GO:0008285 TAS
    protein phosphorylation GO:0006468 IDA
    regulation of translational initiation by eIF2 alpha phosphorylation GO:0010998 TAS
    modulation by virus of host process GO:0019054 TAS
    response to virus GO:0009615 IMP
    endoplasmic reticulum unfolded protein response GO:0030968 IEA
    regulation of hematopoietic stem cell differentiation GO:1902036 ISS
    regulation of NLRP3 inflammasome complex assembly GO:1900225 ISS
    activation of MAPKK activity GO:0000186 IMP
    viral life cycle GO:0019058 TAS
    regulation of protein phosphatase type 2A activity GO:0034047 TAS
    regulation of hematopoietic stem cell proliferation GO:1902033 ISS
Subcellular Localization
    membrane GO:0016020 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    ribosome GO:0005840 TAS
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified EIF2AK2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2AK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDGFRB 5159
Invivo Homo sapiens
2 METAP2 10988
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 ILF2 3608
Invitro Homo sapiens
Invivo Homo sapiens
4 PTGES3 10728
Invitro Homo sapiens
Invivo Homo sapiens
5 PPP1CA 5499
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
6 STAT3 6774
Invivo Homo sapiens
7 DNAJC3 5611
Reconstituted Complex Homo sapiens
8 PPP2R5A  
Affinity Capture-Western Homo sapiens
9 MAP3K5  
Invivo Homo sapiens
Invitro Homo sapiens
10 DBNL 28988
Affinity Capture-MS Homo sapiens
11 STAT1 6772
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
12 PRKRA 8575
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 IKBKB 3551
Invivo Homo sapiens
Invitro Homo sapiens
14 ILF3 3609
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
15 HSP90AA1 3320
Invivo Homo sapiens
16 FANCC 2176
Affinity Capture-Western Homo sapiens
17 PRKRIP1 79706
Affinity Capture-Western Homo sapiens
18 TARBP2  
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 TP53  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which EIF2AK2 is involved
PathwayEvidenceSource
Inhibition of PKR TAS Reactome
ISG15 antiviral mechanism TAS Reactome





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