Gene description for ADAR
Gene name adenosine deaminase, RNA-specific
Gene symbol ADAR
Other names/aliases ADAR1
AGS6
DRADA
DSH
DSRAD
G1P1
IFI-4
IFI4
K88DSRBP
P136
Species Homo sapiens
 Database cross references - ADAR
ExoCarta ExoCarta_103
Entrez Gene 103
HGNC 225
MIM 146920
UniProt P55265  
 ADAR identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for ADAR
Molecular Function
    double-stranded RNA adenosine deaminase activity GO:0003726 NAS
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    poly(A) RNA binding GO:0044822 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    negative regulation of apoptotic process GO:0043066 IEA
    adenosine to inosine editing GO:0006382 TAS
    somatic diversification of immune receptors via somatic mutation GO:0002566 IEA
    mRNA modification GO:0016556 TAS
    definitive hemopoiesis GO:0060216 IEA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    innate immune response GO:0045087 TAS
    pre-miRNA processing GO:0031054 IDA
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 IMP
    cytokine-mediated signaling pathway GO:0019221 TAS
    base conversion or substitution editing GO:0016553 IDA
    protein export from nucleus GO:0006611 IDA
    mRNA processing GO:0006397 IEA
    in utero embryonic development GO:0001701 IEA
    negative regulation of RNA interference GO:1900369 IEA
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IEA
    miRNA loading onto RISC involved in gene silencing by miRNA GO:0035280 IDA
    positive regulation of viral genome replication GO:0045070 IMP
    erythrocyte differentiation GO:0030218 IEA
    response to interferon-alpha GO:0035455 IDA
    defense response to virus GO:0051607 IEA
    osteoblast differentiation GO:0001649 IEA
    protein import into nucleus GO:0006606 IDA
    response to virus GO:0009615 IMP
    gene expression GO:0010467 TAS
    type I interferon signaling pathway GO:0060337 TAS
    hematopoietic stem cell homeostasis GO:0061484 IEA
    negative regulation of viral genome replication GO:0045071 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    nucleoplasm GO:0005654 TAS
    supraspliceosomal complex GO:0044530 IDA
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 TAS
    nucleus GO:0005634 TAS
 Experiment description of studies that identified ADAR in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADAR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XPO1 7514
Invitro Homo sapiens
Invivo Homo sapiens
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